alpha Internexin antibody

Name: alpha Internexin antibody
Brand: GeneTex
SKU: GTX133356
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-alpha Internexin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133356

Anti-alpha Internexin antibody used in IHC (Paraffin sections) (IHC-P). GTX133356

Anti-alpha Internexin antibody used in IHC (Frozen sections) (IHC-Fr). GTX133356

Anti-alpha Internexin antibody used in Western Blot (WB). GTX133356

Cat. No. GTX133356

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr
Reactivity	Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all alpha Internexin products

Datasheet
Document

View product citations for antibody GTX133356 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	1:100-1:1000

Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

mouse brain , rat brain

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of mouse alpha Internexin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886952

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

internexin neuronal intermediate filament protein, alpha , AV028420 , NF-66 , NF66 , alpha-Inx

Background

Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.

Database

Gene ID: 226180 Ina
UniProt: P46660 Ina

Research Area

DATA IMAGES

GTX133356 ICC/IF Image

alpha Internexin antibody detects alpha Internexin protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Internexin protein stained by alpha Internexin antibody (GTX133356) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX133356 IHC-P Image

alpha Internexin antibody detects alpha Internexin protein at neurofilament in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
alpha Internexin antibody (GTX133356) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX133356 IHC-Fr Image

alpha Internexin antibody detects alpha Internexin protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: alpha Internexin protein stained by alpha Internexin antibody (GTX133356) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

GTX133356 IHC-P Image

alpha Internexin antibody detects alpha Internexin protein at neurofilament in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
alpha Internexin antibody (GTX133356) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX133356 WB Image

Various tissue extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody (GTX133356) diluted at 1:3000.The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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