GeneTex
United States (US)

alpha SNAP antibody [4E4]

Cat No. GTX16391

Host Mouse
Clonality Monoclonal
Clone Name 4E4
Isotype IgG1
Application WB, IP, ELISA
Reactivity Human, Rat
APPLICATION

Application Note

ELISA: Use at an assay dependent dilution. IP: Use at a concentration of 2 - 10 μg/ml. WB: Use at a concentration of 0.5 - 5 μg/ml. Detects a band of approximately 36 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

33 kDa. ( Note )

Positive Control

Rat kidney, brain and MDBK cells

Specificity/Sensitivity

Does not cross react with beta-SNAP.
PROPERTIES

Form

Liquid

Buffer

Preservative: NoneConstituents: 50% Glycerol, 0.15M Sodium chloride, 0.02M Sodium phosphate. pH 7.5

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full length Human alpha SNAP.

Purification

IgG fraction
The antibody was purified to >95% homogeneity by standard chromatographic techniques.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nsf Attachment Protein Alpha,Snapa,Napa

Cellular Localization

Cytoplasmic peripheral membrane protein

Background

SNAPs (soluble NSF attachment proteins), acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. Vesicle-to-target membrane docking (initial contact) occurs when the vesicle SNARE binds to its cognate target membrane SNAP. a-SNAP (or b-SNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process. alpha SNAP is expressed in all mammalian tissues.

Database

Research Area

Package List Price ($)
$ 329