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alpha Skeletal Muscle Actin antibody

Anti-alpha Skeletal Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX101362
Anti-alpha Skeletal Muscle Actin antibody used in Western Blot (WB). GTX101362
Anti-alpha Skeletal Muscle Actin antibody used in Western Blot (WB). GTX101362

Cat No. GTX101362

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, FACS
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
FACS Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

Raji , NIH-3T3 , JC , BCL-1 , C2C12

Predict Reactivity

Rat, Bovine, Chicken, Pig, Japanese Medaka, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Skeletal Muscle Actin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

actin alpha 1, skeletal muscle,ACTA,ASMA,CFTD,CFTD1,CFTDM,MPFD,NEM1,NEM2,NEM3,SHPM

Cellular Localization

Cytoplasm , cytoskeleton

Background

The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008]

Database

  • Gene ID: 58 ACTA1
  • UniProt: P68133 ACTA1

Research Area

DATA IMAGES
Anti-alpha Skeletal Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX101362

GTX101362 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human skeletal muscle, using alpha Actin (skeletal muscle)(GTX101362) antibody(10 μg/ml).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Skeletal Muscle Actin antibody used in Western Blot (WB). GTX101362

GTX101362 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
D: C2C12
10% SDS PAGE
GTX101362 diluted at 1:5000

Anti-alpha Skeletal Muscle Actin antibody used in Western Blot (WB). GTX101362

GTX101362 WB Image

Sample(30 μg of whole cell lysate)
A:Raji(GTX27908)
10% SDS PAGE
GTX101362 diluted at 1:1000

REFERENCE
REVIEW

alpha Skeletal Muscle Actin antibody Cat No. GTX101362

Rating ( Average 5 based on 1 users reviews)
Application
Flow cytometry(FACS) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 26-Sep-2016
Score :
Application Tested : FACS
Sample Species : Hu
Sample : HT29 cells
Blocking : 1% skim milk, 4°C, 1Hr
Fixation : 70% EtOH, -20°C, 1Hr
Permeabilization /Antigen retrieval : 70% EtOH, -20°C, 1hrHr
Primary Antibody : 1:200, 4°C, 1Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169