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alpha Smooth Muscle Actin antibody [0.N.5]

Anti-alpha Smooth Muscle Actin antibody [0.N.5] used in IHC (Paraffin sections) (IHC-P). GTX18147

Cat. No. GTX18147

Reference 7

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 0.N.5

Isotype

 IgG2a

Application

 ICC/IF, IHC-P, IHC

Reactivity

 Human, Mouse, Rabbit
Package
250 μl ($399)
See all alpha Smooth Muscle Actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-P 1:800 for 20 minutes at RT
IHC Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Product Note

Recognizes human alpha-smooth muscle isoform of actin. Stains smooth muscle cells in vessel walls, gut wall and myometrium. Myoepithelial cells in breast and salivary gland are also stained. Reacts with tumors arising from smooth muscles and myoepithelial cells.

PROPERTIES

Form

Liquid

Buffer

PBS pH7.4, 0.2% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

N-terminal decapeptide of the alpha smooth muscle isoform of Actin; acetylated at the N-terminus. Epitope: Acetyl group and the first 4 amino acids on the N-terminal end of the peptide chain of alpha-smooth Actin.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_425255

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AAT6 , ACTA2 , ACTSA , MYMY5 , actin, alpha 2, smooth muscle, aorta , SMA , alpha smooth muscle Actin , alpha sma , α-SMA , a-SMA , α_SMA , actin alpha 2, smooth muscle

Cellular Localization

Cytoplasm,Cytoskeleton

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome. [provided by RefSeq, Sep 2017]

Database

  • Gene ID: 59 ACTA2
  • UniProt: P62736 ACTA2

Research Area

DATA IMAGES

Anti-alpha Smooth Muscle Actin antibody [0.N.5] used in IHC (Paraffin sections) (IHC-P). GTX18147

GTX18147 IHC-P Image

IHC-P analysis of human leiomyoma tissue using GTX18147 alpha Smooth Muscle Actin antibody [0.N.5].

REFERENCE

Application Reference

Taghizadeh H et al. J Pers Med 2022; 12 (10) Deteriorated Vascular Homeostasis in Hypertension: Experimental Evidence from Aorta, Brain, and Pancreatic Vasculature.
Application : IHC-P
Reactivity : Rabbit
Xie X et al. Front Bioeng Biotechnol 2022; 10 : 1066266 Vascular endothelial growth factor attenuates neointimal hyperplasia of decellularized small-diameter vascular grafts by modulating the local inflammatory response.
Application : IHC-P
Reactivity : Rabbit
Chen C et al. Front Bioeng Biotechnol 2022; 10 : 894956 A proteomics analysis of neointima formation on decellularized vascular grafts reveals regenerative alterations in protein signature running head: Proteomics analysis of neointima formation.
Yuhong Liu et al. Front Bioeng Biotechnol 2022; 10 : 816513 Photooxidation and Pentagalloyl Glucose Cross-Linking Improves the Performance of Decellularized Small-Diameter Vascular Xenograft In Vivo.
Riggle BA et al. J Clin Invest 2019; (Epub) CD8+ T cells target cerebrovasculature in children with cerebral malaria.
Application : IHC-P
Reactivity : Human
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REVIEW

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Application Reference
Taghizadeh H et al. J Pers Med 2022; 12 (10) Deteriorated Vascular Homeostasis in Hypertension: Experimental Evidence from Aorta, Brain, and Pancreatic Vasculature.
Application : IHC-P
Reactivity : Rabbit
Xie X et al. Front Bioeng Biotechnol 2022; 10:1066266 Vascular endothelial growth factor attenuates neointimal hyperplasia of decellularized small-diameter vascular grafts by modulating the local inflammatory response.
Application : IHC-P
Reactivity : Rabbit
Chen C et al. Front Bioeng Biotechnol 2022; 10:894956 A proteomics analysis of neointima formation on decellularized vascular grafts reveals regenerative alterations in protein signature running head: Proteomics analysis of neointima formation.
Yuhong Liu et al. Front Bioeng Biotechnol 2022; 10:816513 Photooxidation and Pentagalloyl Glucose Cross-Linking Improves the Performance of Decellularized Small-Diameter Vascular Xenograft In Vivo.
Riggle BA et al. J Clin Invest 2019; (Epub) CD8+ T cells target cerebrovasculature in children with cerebral malaria.
Application : IHC-P
Reactivity : Human
Otto C et al. Exp Ther Med 2012; 3 (3):410-414 Use of a murine endometriosis interna model for the characterization of compounds that effectively treat human endometriosis.
Application : IHC-P
Reactivity : Mouse
Klarquist J et al. Am J Pathol 2009; 175 (6):2463-72 Melanoma-associated antigen expression in lymphangioleiomyomatosis renders tumor cells susceptible to cytotoxic T cells.
Application : IHC
Reactivity : Human
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399