For IF: Use at 10-20 μg/ml for 2 hours at room temperature. For IF: paraffin-embedded or frozen sections, as well as on cell smears can be used. Optimal dilutions/concentrations should be determined by the end user.
This antibody reacts with the alpha-smooth muscle isoform of actin. It does not react with actin from fibroblasts (beta- and gamma-cytoplasmic), striated muscle (alpha-sarcomeric) and myocardium (alpha-myocardial).
50 mM potassium phosphate, 150 mM Sodium Chloride, 10mg/ml BSA, carrier polysaccharides, and 0.05% Sodium Azide, pH 7.2.
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
The N-terminal decapeptide of alpha-smooth muscle actin
Fluorescein isothiocyanate (FITC)
_x000B_Absorption : 495 nm_x000B_ ; Emission : 528 nm._x000B_ Ratio : 0.7 molecules FITC per Mouse IgG molecule.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Actin, Alpha 2, Smooth Muscle, Aorta , Actsa , Acta2
The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]