Application Note
For IF: Use at 10-20 μg/ml for 2 hours at room temperature. For IF: paraffin-embedded or frozen sections, as well as on cell smears can be used. Optimal dilutions/concentrations should be determined by the end user.
Calculated MW
Positive Control
Bowel
Product Note
This antibody reacts with the alpha-smooth muscle isoform of actin. It does not react with actin from fibroblasts (beta- and gamma-cytoplasmic), striated muscle (alpha-sarcomeric) and myocardium (alpha-myocardial).
Form
Liquid
Buffer
50mM Potassium Phosphate, 150mM NaCl, 1% BSA, carrier polysaccharides
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
Antigen Species
Human
Immunogen
The N-terminal decapeptide of alpha-smooth muscle actin
Purification
Unpurified
Conjugation
Fluorescein isothiocyanate (FITC)
_x000B_Absorption : 495 nm_x000B_ ; Emission : 528 nm._x000B_ Ratio : 0.7 molecules FITC per Mouse IgG molecule.
RRID
AB_383662
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
actin alpha 2, smooth muscle , ACTSA
Cellular Localization
Cytoplasm, cytoskeleton
Background
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome. [provided by RefSeq, Sep 2017]
Database
Research Area