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alpha Smooth Muscle Actin antibody [1A4] (FITC)

Cat No. GTX72531

Host

Mouse

Clonality

Monoclonal

Clone Name

1A4

Isotype

IgG2a

Application

ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

Human, Mouse, Rat, Rabbit, Guinea pig, Baboon
Package
100 μg ($319)

APPLICATION

Application Note

For IF: Use at 10-20 μg/ml for 2 hours at room temperature. For IF: paraffin-embedded or frozen sections, as well as on cell smears can be used. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

42 kDa. ( Note )

Positive Control

Bowel

Specificity/Sensitivity

This antibody reacts with the alpha-smooth muscle isoform of actin. It does not react with actin from fibroblasts (beta- and gamma-cytoplasmic), striated muscle (alpha-sarcomeric) and myocardium (alpha-myocardial).

PROPERTIES

Form

Liquid

Buffer

50 mM potassium phosphate, 150 mM Sodium Chloride, 10mg/ml BSA, carrier polysaccharides, and 0.05% Sodium Azide, pH 7.2.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Antigen Species

Human

Immunogen

The N-terminal decapeptide of alpha-smooth muscle actin

Purification

Unpurified

Conjugation

Fluorescein isothiocyanate (FITC)
_x000B_Absorption : 495 nm_x000B_ ; Emission : 528 nm._x000B_ Ratio : 0.7 molecules FITC per Mouse IgG molecule.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Actin, Alpha 2, Smooth Muscle, Aorta , Actsa , Acta2

Cellular Localization

Cytoplasmic

Background

The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]

Database

Research Area

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 319