APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1/500 - 1/2000 |
1/200 - 1/1000 |
1/200 - 1/400 |
1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.03% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified recombinant fragment of human ACTA2 expressed in E. Coli.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
actin alpha 2, smooth muscle , ACTSA
Cellular Localization
Cytoplasm, cytoskeleton
Background
The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
Database
Research Area
DATA IMAGES
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GTX60393 ELISA Image
ELISA analysis of antigen using GTX60393 alpha Smooth Muscle Actin antibody [1H8].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng
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GTX60393 FACS Image
FACS analysis of HeLa cells using GTX60393 alpha Smooth Muscle Actin antibody [1H8]. Green : alpha Smooth Muscle Actin Purple : negative control
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GTX60393 ICC/IF Image
ICC/IF analysis of NIH3T3 cells using GTX60393 alpha Smooth Muscle Actin antibody [1H8]. Green : alpha Smooth Muscle Actin Blue: DRAQ5 fluorescent DNA dye
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GTX60393 WB Image
WB analysis of HeLa (1), Jurkta (2), HepG2 (3), MCF-7 (4), A431 (5), A549 (6), PC-12 (7), NIH3T3 (8) and Cos7 (9) cell lysate using GTX60393 alpha Smooth Muscle Actin antibody [1H8].
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REFERENCE
There are currently no references for alpha Smooth Muscle Actin antibody [1H8] (GTX60393). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for alpha Smooth Muscle Actin antibody [1H8] (GTX60393). Be the first to share your experience with this product.