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alpha Smooth Muscle Actin antibody [6A12]

Anti-alpha Smooth Muscle Actin antibody [6A12] used in Western Blot (WB). GTX34111
Anti-alpha Smooth Muscle Actin antibody [6A12] used in IHC (Paraffin sections) (IHC-P). GTX34111
Anti-alpha Smooth Muscle Actin antibody [6A12] used in IHC (Paraffin sections) (IHC-P). GTX34111
Anti-alpha Smooth Muscle Actin antibody [6A12] used in IHC (Paraffin sections) (IHC-P). GTX34111
Anti-alpha Smooth Muscle Actin antibody [6A12] used in IHC (Paraffin sections) (IHC-P). GTX34111
Anti-alpha Smooth Muscle Actin antibody [6A12] used in IHC (Paraffin sections) (IHC-P). GTX34111
Anti-alpha Smooth Muscle Actin antibody [6A12] used in IHC (Paraffin sections) (IHC-P). GTX34111

Cat No. GTX34111

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6A12

Isotype

 IgG1

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($319)
See all alpha Smooth Muscle Actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:10000
IHC-P 1:1000-1:2000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic Peptide

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

AAT6,ACTA2,ACTSA,MYMY5,actin, alpha 2, smooth muscle, aorta,SMA,alpha smooth muscle Actin,alpha sma,α-SMA,a-SMA,α_SMA,actin alpha 2, smooth muscle

Cellular Localization

Cytoplasm,Cytoskeleton

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome. [provided by RefSeq, Sep 2017]

Database

  • Gene ID: 59 ACTA2
  • UniProt: P62736 ACTA2

Research Area

DATA IMAGES

Anti-alpha Smooth Muscle Actin antibody [6A12] used in Western Blot (WB). GTX34111

GTX34111 WB Image

WB analysis of various sample lysates using GTX34111 alpha Smooth Muscle Actin antibody [6A12].
Lane 1 : HeLa cell lysate
Lane 2 : 3T3 cell lysate
Lane 3 : Rat brain tissue lysate

Anti-alpha Smooth Muscle Actin antibody [6A12] used in IHC (Paraffin sections) (IHC-P). GTX34111

GTX34111 IHC-P Image

IHC-P analysis of human stomach tissue using GTX34111 alpha Smooth Muscle Actin antibody [6A12]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

Anti-alpha Smooth Muscle Actin antibody [6A12] used in IHC (Paraffin sections) (IHC-P). GTX34111

GTX34111 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX34111 alpha Smooth Muscle Actin antibody [6A12]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

Anti-alpha Smooth Muscle Actin antibody [6A12] used in IHC (Paraffin sections) (IHC-P). GTX34111

GTX34111 IHC-P Image

IHC-P analysis of human uterus cancer tissue using GTX34111 alpha Smooth Muscle Actin antibody [6A12]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

Anti-alpha Smooth Muscle Actin antibody [6A12] used in IHC (Paraffin sections) (IHC-P). GTX34111

GTX34111 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX34111 alpha Smooth Muscle Actin antibody [6A12]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

Anti-alpha Smooth Muscle Actin antibody [6A12] used in IHC (Paraffin sections) (IHC-P). GTX34111

GTX34111 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX34111 alpha Smooth Muscle Actin antibody [6A12]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

Anti-alpha Smooth Muscle Actin antibody [6A12] used in IHC (Paraffin sections) (IHC-P). GTX34111

GTX34111 IHC-P Image

IHC-P analysis of mouse cecal tissue using GTX34111 alpha Smooth Muscle Actin antibody [6A12].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319