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alpha Smooth Muscle Actin antibody [6A12]

Anti-alpha Smooth Muscle Actin antibody [6A12] used in Western Blot (WB). GTX34111
Anti-alpha Smooth Muscle Actin antibody [6A12] used in IHC (Paraffin sections) (IHC-P). GTX34111

Cat No. GTX34111

Host Mouse
Clonality Monoclonal
Clone Name 6A12
Isotype IgG1
Application WB, IHC-P
Reactivity Human, Mouse, Rat
See all alpha Smooth Muscle Actin products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Specificity/Sensitivity

The antibody detects endogenous α-SMA protein.
PROPERTIES

Form

Liquid

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic Peptide

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Actin, Alpha 2, Smooth Muscle, Aorta,Actsa,Acta2

Background

The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]

Database

Research Area

DATA IMAGES
Anti-alpha Smooth Muscle Actin antibody [6A12] used in Western Blot (WB). GTX34111

GTX34111 WB Image

Western Blot (WB) analysis of 1) HeLa, 2) 3T3, 3) Rat Brain using alpha-SMA Monoclonal Antibody.
Anti-alpha Smooth Muscle Actin antibody [6A12] used in IHC (Paraffin sections) (IHC-P). GTX34111

GTX34111 IHC-P Image

Immunohistochemistry (IHC) analysis of paraffin-embedded Mouse Cecal Tissue using alpha-SMA Monoclonal Antibody.
Package List Price ($)
$ 319