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alpha Smooth Muscle Actin antibody [AT132D3]

Anti-alpha Smooth Muscle Actin antibody [AT132D3] used in Flow cytometry (FACS). GTX57619
Anti-alpha Smooth Muscle Actin antibody [AT132D3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57619
Anti-alpha Smooth Muscle Actin antibody [AT132D3] used in Western Blot (WB). GTX57619

Cat. No. GTX57619

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT132D3

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μl ($399)
See all alpha Smooth Muscle Actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Recommended starting dilution is 1:1000.
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT132D3 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ACTA4 protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin alpha 2, smooth muscle , AAT6 , ACTA2 , ACTSA , MYMY5 , actin, alpha 2, smooth muscle, aorta , SMA , alpha smooth muscle Actin , alpha sma , α-SMA , a-SMA , α_SMA

Cellular Localization

Cytoplasm,Cytoskeleton

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome. [provided by RefSeq, Sep 2017]

Database

  • Gene ID: 59 ACTA2
  • UniProt: P62736 ACTA2

Research Area

DATA IMAGES

Anti-alpha Smooth Muscle Actin antibody [AT132D3] used in Flow cytometry (FACS). GTX57619

GTX57619 FACS Image

FACS analysis of Nb2-11 cells using GTX57619 alpha Smooth Muscle Actin antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg

Anti-alpha Smooth Muscle Actin antibody [AT132D3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57619

GTX57619 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX57619 alpha Smooth Muscle Actin antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

Anti-alpha Smooth Muscle Actin antibody [AT132D3] used in Western Blot (WB). GTX57619

GTX57619 WB Image

WB analysis of various samples using GTX57619 alpha Smooth Muscle Actin antibody.
Lane 1 : HepG2 whole cell lysate
Lane 2 : SW480 whole cell lysate
Lane 3 : HeLa whole cell lysate
Loading : 40 μg
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399