alpha Smooth Muscle Actin antibody [GT445]

Name: alpha Smooth Muscle Actin antibody [GT445]
Brand: GeneTex
SKU: GTX629702
Price: 329.00 USD
Rating: 5 (1 reviews)

Anti-alpha Smooth Muscle Actin antibody [GT445] used in Western Blot (WB). GTX629702

Anti-alpha Smooth Muscle Actin antibody [GT445] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629702

Anti-alpha Smooth Muscle Actin antibody [GT445] used in IHC (Paraffin sections) (IHC-P). GTX629702

Cat No. GTX629702

Reference 6

Host	Mouse
Clonality	Monoclonal
Clone Name	GT445
Isotype	IgG1
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat, Drosophila
Package	100 μl ($329), 25 μl ($169)

See all alpha Smooth Muscle Actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:10000
ICC/IF	1:100-1:1000
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , Rat-2 , drosophila

Predict Reactivity

Zebrafish, Rabbit, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Smooth Muscle Actin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888137

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

actin alpha 2, smooth muscle , ACTSA

Cellular Localization

Cytoplasm , cytoskeleton

Background

The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]

Database

Gene ID: 59 ACTA2
UniProt: P62736 ACTA2

Research Area

DATA IMAGES

GTX629702 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody [GT445] (GTX629702) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629702 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody [GT445] (GTX629702) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629702 WB Image

alpha smooth muscle Actin antibody [GT445] detects alpha smooth muscle Actin protein by western blot analysis.
A. 30 μg C8D30 whole cell lysate/extract
B. 30 μg NIH-3T3 whole cell lysate/extract
C. 30 μg BCL-1 whole cell lysate/extract
D. 30 μg Raw264.7 whole cell lysate/extract
E. 30 μg C2C12 whole cell lysate/extract
10% SDS-PAGE
alpha smooth muscle Actin antibody [GT445] (GTX629702) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629702 ICC/IF Image

alpha Smooth Muscle Actin antibody [GT445] detects alpha Smooth Muscle Actin protein at cytoplasm and cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Smooth Muscle Actin protein stained by alpha Smooth Muscle Actin antibody [GT445] (GTX629702) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX629702 WB Image

alpha smooth muscle Actin antibody [GT445] detects alpha smooth muscle Actin protein by western blot analysis.
A. 30 μg drosophila lysate/extract
10% SDS-PAGE
alpha smooth muscle Actin antibody [GT445] (GTX629702) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629702 WB Image

alpha smooth muscle Actin antibody [GT445] detects alpha smooth muscle Actin protein by western blot analysis.
A. 30 μg Rat-2 whole cell lysate/extract
10% SDS-PAGE
alpha smooth muscle Actin antibody [GT445] (GTX629702) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX629702 IHC-P Image

alpha Smooth Muscle Actin antibody [GT445] detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody [GT445] (GTX629702) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX629702 IHC-P Image

alpha Smooth Muscle Actin antibody [GT445] detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody [GT445] (GTX629702) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX629702 IHC-P Image

alpha Smooth Muscle Actin antibody [GT445] detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis (Autostainer Formulated).
Sample: Paraffin-embedded human breast cancer.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody [GT445] (GTX629702) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, 20 min

REFERENCE

Application Reference

Ho YJ et al. Theranostics 2019; 9 (24) : 7370-7383 Normalization of Tumor Vasculature by Oxygen Microbubbles with Ultrasound.

Application : IHC-P

Reactivity : Mouse

Xu D et al. Eur Rev Med Pharmacol Sci 2018; 22 (21) : 7431-7438 Effects of miR-155 on hypertensive rats via regulating vascular mesangial hyperplasia.

Application : WB

Reactivity : Rat

Shih YC et al. Circ Res 2018; (Epub) Endoplasmic Reticulum Protein TXNDC5 Augments Myocardial Fibrosis by Facilitating Extracellular Matrix Protein Folding and Redox-Sensitive Cardiac Fibroblast Activation.

Application : ICC/IF

Reactivity : Human

Liu MC et al. PLoS One 2017; 12 (5) : e0178152 Inhibition of chronic prostate inflammation by hyaluronic acid through an immortalized human prostate stromal cell line model.

Application : ICC/IF

Reactivity : Human

Davide P et al. Cell Mol Gastroenterol Hepatol 2015; 1 (6) : 646-663 Lipid-Induced Hepatocyte-Derived Extracellular Vesicles Regulate Hepatic Stellate Cells via MicroRNA Targeting Peroxisome Proliferator-Activated Receptor γ.

Application : WB

Reactivity : Human

Show More +

Submit a Reference

REVIEW