APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500-1:10000 |
1:100-1:1000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , Rat-2 , drosophila
Predict Reactivity
Zebrafish, Rabbit, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.75 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human alpha Smooth Muscle Actin. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888137
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
actin alpha 2, smooth muscle , ACTSA
Cellular Localization
Cytoplasm , cytoskeleton
Background
The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
Database
Research Area
DATA IMAGES
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GTX629702 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody [GT445] (GTX629702) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX629702 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody [GT445] (GTX629702) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX629702 WB Image
alpha smooth muscle Actin antibody [GT445] detects alpha smooth muscle Actin protein by western blot analysis. A. 30 μg C8D30 whole cell lysate/extract B. 30 μg NIH-3T3 whole cell lysate/extract C. 30 μg BCL-1 whole cell lysate/extract D. 30 μg Raw264.7 whole cell lysate/extract E. 30 μg C2C12 whole cell lysate/extract 10% SDS-PAGE alpha smooth muscle Actin antibody [GT445] (GTX629702) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX629702 ICC/IF Image
alpha Smooth Muscle Actin antibody [GT445] detects alpha Smooth Muscle Actin protein at cytoplasm and cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Smooth Muscle Actin protein stained by alpha Smooth Muscle Actin antibody [GT445] (GTX629702) diluted at 1:500. Blue: Hoechst 33342 staining.
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GTX629702 WB Image
alpha smooth muscle Actin antibody [GT445] detects alpha smooth muscle Actin protein by western blot analysis. A. 30 μg drosophila lysate/extract 10% SDS-PAGE alpha smooth muscle Actin antibody [GT445] (GTX629702) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX629702 WB Image
alpha smooth muscle Actin antibody [GT445] detects alpha smooth muscle Actin protein by western blot analysis. A. 30 μg Rat-2 whole cell lysate/extract 10% SDS-PAGE alpha smooth muscle Actin antibody [GT445] (GTX629702) dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX629702 IHC-P Image
alpha Smooth Muscle Actin antibody [GT445] detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse duodenum.alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody [GT445] (GTX629702) diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX629702 IHC-P Image
alpha Smooth Muscle Actin antibody [GT445] detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat stomach.alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody [GT445] (GTX629702) diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX629702 IHC-P Image
alpha Smooth Muscle Actin antibody [GT445] detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis (Autostainer Formulated).Sample: Paraffin-embedded human breast cancer.alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody [GT445] (GTX629702) diluted at 1:2000.Antigen Retrieval: Citrate buffer, 20 min
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REFERENCE
REVIEW
alpha Smooth Muscle Actin antibody [GT445]
Cat No. GTX629702
Rating
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( Average 5 based on 1 users reviews)
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Application
IHC (Paraffin sections)(IHC-P)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 21-Oct-2020
Application Tested :
IHC-P
Sample Species :
Hu
Sample :
breast cancer tissue
Fixation :
Citrate buffer, 0.3°C
Primary Antibody :
1:2000, 4°C, 16Hr