alpha Smooth Muscle Actin antibody, N-term

WB analysis of human (A) and rat (B) duodenum lysate using GTX89701 alpha Smooth Muscle Actin antibody, N-term.
Dilution : 0.1-0.5μg/ml
Loading : 35μg protein in RIPA buffer

ICC/IF analysis of PFA fixed HepG2 cells using GTX89701 alpha Smooth Muscle Actin antibody, N-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

WB analysis of human (A) and mouse (B) heart lysate (35μg protein in RIPA buffer) using GTX89701 alpha Smooth Muscle Actin antibody, N-term.
Dilution : 0.1μg/ml (human heart) and 0.3μg/ml (mouse heart)

WB analysis of HeLa (A) and NIH3T3 (B) cell lysate (35μg protein in RIPA buffer) using GTX89701 alpha Smooth Muscle Actin antibody, N-term.
Dilution : 0.3μg/ml (HeLa) and 1μg/ml (NIH3T3)

IHC-P analysis of human lung using GTX89701 alpha Smooth Muscle Actin antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.5μg/ml

The data was published in the journal PLoS One in 2014. PMID: 25541982

The data was published in the journal J Biomed Sci in 2014. PMID: 24606563