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alpha Smooth Muscle Actin antibody, N-term

Anti-alpha Smooth Muscle Actin antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX89701
Anti-alpha Smooth Muscle Actin antibody, N-term used in Western Blot (WB). GTX89701
Anti-alpha Smooth Muscle Actin antibody, N-term used in Western Blot (WB). GTX89701
Anti-alpha Smooth Muscle Actin antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89701
Anti-alpha Smooth Muscle Actin antibody, N-term used in Western Blot (WB). GTX89701
Anti-alpha Smooth Muscle Actin antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89701
Anti-alpha Smooth Muscle Actin antibody, N-term used in IHC (Frozen sections) (IHC-Fr). GTX89701

Cat. No. GTX89701

Reference 5

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all alpha Smooth Muscle Actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5μg/ml
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89701-PEP).

Predict Reactivity

Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence EEEDSTALVC, from the N Terminus of the protein sequence according to NP_001604.1; NP_001135417.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10721877

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin alpha 2, smooth muscle , ACTSA

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome. [provided by RefSeq, Sep 2017]

Database

  • Gene ID: 59 ACTA2
  • UniProt: P62736 ACTA2

Research Area

DATA IMAGES

Anti-alpha Smooth Muscle Actin antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX89701

GTX89701 IHC-P Image

IHC-P analysis of human lung using GTX89701 alpha Smooth Muscle Actin antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.5μg/ml

Anti-alpha Smooth Muscle Actin antibody, N-term used in Western Blot (WB). GTX89701

GTX89701 WB Image

WB analysis of HeLa (A) and NIH3T3 (B) cell lysate (35μg protein in RIPA buffer) using GTX89701 alpha Smooth Muscle Actin antibody, N-term.
Dilution : 0.3μg/ml (HeLa) and 1μg/ml (NIH3T3)

Anti-alpha Smooth Muscle Actin antibody, N-term used in Western Blot (WB). GTX89701

GTX89701 WB Image

WB analysis of human (A) and mouse (B) heart lysate (35μg protein in RIPA buffer) using GTX89701 alpha Smooth Muscle Actin antibody, N-term.
Dilution : 0.1μg/ml (human heart) and 0.3μg/ml (mouse heart)

Anti-alpha Smooth Muscle Actin antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89701

GTX89701 ICC/IF Image

ICC/IF analysis of PFA fixed HepG2 cells using GTX89701 alpha Smooth Muscle Actin antibody, N-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

Anti-alpha Smooth Muscle Actin antibody, N-term used in Western Blot (WB). GTX89701

GTX89701 WB Image

WB analysis of human (A) and rat (B) duodenum lysate using GTX89701 alpha Smooth Muscle Actin antibody, N-term.
Dilution : 0.1-0.5μg/ml
Loading : 35μg protein in RIPA buffer

Anti-alpha Smooth Muscle Actin antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89701

GTX89701 ICC/IF Image

The data was published in the journal J Biomed Sci in 2014. PMID: 24606563

Anti-alpha Smooth Muscle Actin antibody, N-term used in IHC (Frozen sections) (IHC-Fr). GTX89701

GTX89701 IHC-Fr Image

The data was published in the journal PLoS One in 2014. PMID: 25541982

REFERENCE

Application Reference

Wu Z et al. EMBO Rep 2023; 24 (4) : e56524 Pericyte stem cells induce Ly6G(+) cell accumulation and immunotherapy resistance in pancreatic cancer.
Application : IHC-P
Reactivity : Mouse
Wang Z et al. Dis Markers 2019; 2019 : 6129748 ADAMTS-5 Decreases in Coronary Arteries and Plasma from Patients with Coronary Artery Disease.
Zhang P et al. Biochem Biophys Res Commun 2017; (Epub) Ischemic flap survival improvement by composition-selective fat grafting with novel adipose tissue derived product - stromal vascular fraction gel.
Application : IHC-P
Reactivity : Mouse
Herrnberger L et al. PLoS One 2014; 9 (12) : e115005 Formation of fenestrae in murine liver sinusoids depends on plasmalemma vesicle-associated protein and is required for lipoprotein passage.
Application : IHC-P
Reactivity : Mouse
Minutoli L et al. J Biomed Sci 2014; 21 (1) : 19 Inhibitors of apoptosis proteins in experimental benign prostatic hyperplasia: effects of serenoa repens, selenium and lycopene.
Application : ICC/IF
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399