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alpha Smooth Muscle Actin antibody

KO/KD Orthogonal
Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034

Cat. No. GTX100034

Reference 99
5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($419),
25 μl ($169)
See all alpha Smooth Muscle Actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

Rat2 , C8D30 , NIH-3T3 , C2C12

Predict Reactivity

Rabbit, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human alpha Smooth Muscle Actin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240408

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin alpha 2, smooth muscle , ACTSA

Cellular Localization

Cytoplasm , cytoskeleton

Background

The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]

Database

  • Gene ID: 59 ACTA2
  • UniProt: P62736 ACTA2

Research Area

DATA IMAGES

Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034

GTX100034 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034

GTX100034 IHC-P Image

alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034

GTX100034 IHC-P Image

alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cervix.
Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034

GTX100034 IHC-P Image

alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis (Autostainer Formulated).
Sample: Paraffin-embedded human normal colon tissue.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:500.
Antigen Retrieval: Citrate buffer, 20 min

Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034

GTX100034 IHC-P Image

alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cervix.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034

GTX100034 IHC-P Image

alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034

GTX100034 WB Image

alpha smooth muscle Actin antibody detects alpha smooth muscle Actin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha smooth muscle Actin antibody (GTX100034) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034

GTX100034 WB Image

alpha smooth muscle Actin antibody detects ACTA2 protein by western blot analysis.
A. 30 μg Rat2 whole cell lysate/extract
10% SDS-PAGE
alpha smooth muscle Actin antibody (GTX100034) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034

GTX100034 IHC-P Image

alpha smooth muscle Actin antibody detects alpha smooth muscle Actin protein at cytoplasm on human liver carcinoma stroma by immunohistochemical analysis.
Sample: Paraffin-embedded liver carcinoma stroma.
alpha smooth muscle Actin antibody (GTX100034) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034

GTX100034 IHC-P Image

alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034

GTX100034 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034

GTX100034 IHC-P Image

alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034

GTX100034 IHC-P Image

alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034

GTX100034 IHC-P Image

alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034

GTX100034 IHC-P Image

alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034

GTX100034 IHC-P Image

alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse smooth muscle.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034

GTX100034 IHC-P Image

alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse smooth muscle.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034

GTX100034 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034

GTX100034 IHC-P Image

alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse smooth muscle.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034

GTX100034 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034

GTX100034 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034

GTX100034 WB Image

Orthogonal

The data was published in the 2022 in J Biomed Sci. PMID: 35765067

Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034

GTX100034 WB Image

Orthogonal

The data was published in the journal Biomolecules in 2019. PMID: 31443530

Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034

GTX100034 WB Image

The data was published in the journal Front Pharmacol in 2016. PMID: 27199755

Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034

GTX100034 IHC-P Image

The data was published in the journal PLoS One in 2019. PMID: 30695033

REFERENCE

Application Reference

CH Chou et al. Int J Biol Sci 2025; 21 (2) : 685-707 The Cyclin-Dependent Kinase 8 Inhibitor E966-0530-45418 Attenuates Pulmonary Fibrosis In Vitro and In Vivo
YF Chiang et al. Redox Biol 2024; 78 : 103411 Regulatory roles of NAMPT and NAD+ metabolism in uterine leiomyoma progression: Implications for ECM accumulation, stemness, and microenvironment
P Sharma Humagain et al. Exp Cell Res 2024; 443 (1) : 114295 Establishment of a genetically amenable fibroblast cell line from Atlantic salmon skin
Fan Y et al. J Biol Chem 2024; 300 (8) : 107516 Role of TSP-1 and its receptor ITGB3 in the renal tubulointerstitial injury of focal segmental glomerulosclerosis.
C Plante et al. PLoS One 2024; 19 (6) : e0301040 Revisiting the NPcis mouse model: A new tool to model plexiform neurofibroma
Application : IHC-P
Show More +
Submit a Reference

REVIEW

alpha Smooth Muscle Actin antibody Cat. No. GTX100034

Rating
( Average 4.4 based on 5 users reviews)
Application
Western Blot(WB)
( Average 4 based on 2 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 4 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 2 users reviews)
Anti-alpha Smooth Muscle Actin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100034
Date : Anonymous submitted on 08-Sep-2022
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : NIH3T3 cells with/without TGFβ1 treatment
Fixation : 100% Methanol
Permeabilization /Antigen retrieval : 2% BSA and 0.2% Triton-X 100 in PBS
Primary Antibody : 1:1000, 4°C, 24Hr
Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034
Date : Anonymous submitted on 12-Oct-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : mouse uterus tissue
Blocking : 2% Donkey Serum, 1%BSA, 0.1% coldfish skin gelatin, 0.1% Triton-X, 0.05% Tween-20 in 1xPBS, RT, 1Hr
Fixation : 4%PFA
Permeabilization /Antigen retrieval : PBS-T (0.05% Tween20) / 10mM Citrate buffer (pH6.0)
Primary Antibody : 1:200-800, 4°C, 18Hr
Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034
Date : Anonymous submitted on 21-Oct-2020
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : normal colon tissue
Fixation : Citrate buffer, 0.3°C
Primary Antibody : 1:500, 4°C, 16Hr
Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034
Date : Anonymous submitted on 10-Aug-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : Pancreatic cancer associated fibroblasts
Amount used : 20 μg
Blocking : 5% skim milk in TBST, 25°C, 2Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: Control group, Lane 2: Treated with a compound which decreases the expression of a-SMA at a transcriptional level.
Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034
Date : Anonymous submitted on 04-May-2012
Score :
Application Tested : WB
Sample Species : Ms
Sample : Mouse heart tssue
Amount used : 20μg
Blocking : 5% milk, 25°C, 2Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Submit a Review
Application Reference
CH Chou et al. Int J Biol Sci 2025; 21 (2):685-707 The Cyclin-Dependent Kinase 8 Inhibitor E966-0530-45418 Attenuates Pulmonary Fibrosis In Vitro and In Vivo
YF Chiang et al. Redox Biol 2024; 78:103411 Regulatory roles of NAMPT and NAD+ metabolism in uterine leiomyoma progression: Implications for ECM accumulation, stemness, and microenvironment
P Sharma Humagain et al. Exp Cell Res 2024; 443 (1):114295 Establishment of a genetically amenable fibroblast cell line from Atlantic salmon skin
Fan Y et al. J Biol Chem 2024; 300 (8):107516 Role of TSP-1 and its receptor ITGB3 in the renal tubulointerstitial injury of focal segmental glomerulosclerosis.
C Plante et al. PLoS One 2024; 19 (6):e0301040 Revisiting the NPcis mouse model: A new tool to model plexiform neurofibroma
Application : IHC-P
Gao S et al. Acta Biochim Biophys Sin (Shanghai) 2024; 56 (2):184-198 8-Oxoguanine DNA glycosylase protects cells from senescence via the p53-p21 pathway.
Wang SY et al. Stem Cell Res Ther 2024; 15 (1):113 DPSCs regulate epithelial-T cell interactions in oral submucous fibrosis.
M Rokni et al. Iran J Allergy Asthma Immunol 2024; Interferon-γ Induces Interleukin-6 Production and Alpha-smooth Muscle Actin Expression in Systemic Sclerosis Fibroblasts
B Xue et al. Sci Rep 2024; 14 (1):837 irculating exosomal miR-16-5p and let-7e-5p are associated with bladder fibrosis of diabetic cystopathy
Application : ICC/IF
Kano T et al. Exp Biol Med (Maywood) 2023; 248 (20):1895-1904 The infrapatellar fat pad contributes to spontaneous healing after complete anterior cruciate ligament injury.
Navarro-Gonzalez YD et al. Int J Exp Pathol 2023; 104 (4):209-222 Arginine vasopressin deficiency and conivaptan (a V1a-V2 receptor antagonist) treatment reverses liver damage and fibrosis in rats with chronic portocaval anastomosis.
WC Chiou et al. Biomed Pharmacother 2023; 170:116077 Ergosterol peroxide blocks HDV infection as a novel entry inhibitor by targeting human NTCP receptor
S Wang et al. Research Square 2023; DPSCs Regulate Epithelial-T Cells Interactions in Oral Submucous Fibrosis
Application : ICC/IF, IHC-P
Son B et al. J Nanobiotechnology 2023; 21 (1):310 Secured delivery of basic fibroblast growth factor using human serum albumin-based protein nanoparticles for enhanced wound healing and regeneration.
Garc?a-Arroyo FE et al. Antioxidants (Basel) 2023; 12 (5) Osthole Prevents Heart Damage Induced by Diet-Induced Metabolic Syndrome: Role of Fructokinase (KHK).
Conroy LR et al. Nat Commun 2023; 14 (1):2759 Spatial metabolomics reveals glycogen as an actionable target for pulmonary fibrosis.
Andreucci E et al. Int J Mol Sci 2023; 24 (2) Nintedanib-αVβ6 Integrin Ligand Conjugates Reduce TGFβ-Induced EMT in Human Non-Small Cell Lung Cancer.
Chiou WC et al. Biomed Pharmacother 2023; 161:114481 Ovatodiolide inhibits SARS-CoV-2 replication and ameliorates pulmonary fibrosis through suppression of the TGF-β/TβRs signaling pathway.
Application : WB
Reactivity : Human
Fuentes-Mateos R et al. Methods Protoc 2023; 6 (1) Optimized Protocol for Isolation and Culture of Murine Neonatal Primary Lung Fibroblasts.
Application : ICC/IF
Reactivity : Mouse
Kunitatsu K et al. Int J Mol Sci 2023; 24 (22) Novel Peritoneal Sclerosis Rat Model Developed by Administration of Bleomycin and Lansoprazole.
Yang AM et al. Front Microbiol 2022; 13:887728 Saccharomyces Boulardii Ameliorates Non-alcoholic Steatohepatitis in Mice Induced by a Methionine-Choline-Deficient Diet Through Gut-Liver Axis.
Application : IHC-P
Reactivity : Mouse
Facchinello N et al. Nat Metab 2022; 4 (1):123-140 Oxidative pentose phosphate pathway controls vascular mural cell coverage by regulating extracellular matrix composition.
Jhih-Ying Chi et al. Clin Transl Med. 2022; 12 (11):e1099 Blockade of the pentraxin 3/CD44 interaction attenuates lung injury-induced fibrosis
Application : WB, ICC/IF
Reactivity : Human
Jia-Huang Chen et al. J Biomed Sci 2022; 29 (1):46 The down-regulation of XBP1, an unfolded protein response effector, promotes acute kidney injury to chronic kidney disease transition.
Application : WB
Reactivity : Mouse
Fang Yan et al. BMC Cardiovasc Disord 2022; 22 (1):182 H3K9me2 regulation of BDNF expression via G9a partakes in the progression of heart failure.
Application : WB, IHC-P
Reactivity : Rat
Wenbiao Chen et al. Cell Commun Signal 2022; 20 (1):28 Ameliorating role of microRNA-378 carried by umbilical cord mesenchymal stem cells-released extracellular vesicles in mesangial proliferative glomerulonephritis.
Application : IHC-P
Reactivity : Rat
Lee WJ et al. J Food Drug Anal 2021; 29 (1):113-127 Tea polyphenol epigallocatechin-3-gallate inhibits cell proliferation in a patient-derived triple-negative breast cancer xenograft mouse model via inhibition of proline-dehydrogenase-induced effects.
Qing Hou et al. Front Cell Dev Biol 2021; 9:781792 Nuclear Receptor Interacting Protein-2 Mediates the Stabilization and Activation of β-Catenin During Podocyte Injury.
Application : WB, IHC-P
Reactivity : Human, Mouse
Chen LL et al. Evid Based Complement Alternat Med 2021; 2021:5205759 Suppression of Inflammatory and Fibrotic Signals by Cinnamon (Cinnamomum cassia) and Cinnamaldehyde in Cyclophosphamide-Induced Overactive Bladder in Mice.
Application : IHC-Fr
Reactivity : Mouse
Ballesteros-Martinez C et al. Br J Pharmacol 2021; Microsomal Prostaglandin E Synthase-1 (mPGES-1) is involved in the metabolic and cardiovascular alterations associated with obesity.
Application : WB
Reactivity : Mouse
Kawami M et al. Biomolecules 2021; 11 (12) Characterization of miR-34a-Induced Epithelial-Mesenchymal Transition in Non-Small Lung Cancer Cells Focusing on p53.
Application : WB
Reactivity : Human
Kawami M et al. Toxicology 2021; 461:152903 Evaluation on epithelial-mesenchymal state and microRNAs focusing on isolated alveolar epithelial cells from bleomycin injured rat lung.
Application : WB
Reactivity : Rat
Chiang YF et al. Nutrients 2021; 13 (9) The Role of Cell Proliferation and Extracellular Matrix Accumulation Induced by Food Additive Butylated Hydroxytoluene in Uterine Leiomyoma.
Application : WB
Reactivity : Rat
Prieto-Carrasco R et al. Biology (Basel) 2021; 10 (7) Unilateral Ureteral Obstruction for 28 Days in Rats Is Not Associated with Changes in Cardiac Function or Alterations in Mitochondrial Function.
Application : WB
Reactivity : Rat
Hsieh YC et al. Cell Mol Gastroenterol Hepatol 2021; 12 (3):813-838 (Pro)renin Receptor Knockdown Attenuates Liver Fibrosis Through Inactivation of ERK/TGF-β1/SMAD3 Pathway.
Application : WB
Reactivity : Mouse
Milani M et al. J Neuroinflammation 2021; 18 (1):132 Targeting S100A4 with niclosamide attenuates inflammatory and profibrotic pathways in models of amyotrophic lateral sclerosis.
Application : WB, ICC/IF
Reactivity : Human
Peng M et al. J Ethnopharmacol 2021; 275:114061 Huoxin Pill inhibits isoproterenol-induced transdifferentiation and collagen synthesis in cardiac fibroblasts through the TGF-β/Smads pathway.
Application : IHC-P
Reactivity : Rat
Prieto-Carrasco R et al. Biology (Basel) 2021; 10 (5) Progressive Reduction in Mitochondrial Mass Is Triggered by Alterations in Mitochondrial Biogenesis and Dynamics in Chronic Kidney Disease Induced by 5/6 Nephrectomy.
Application : WB
Liu J et al. J Cell Mol Med 2021; 25 (10):4732-4743 Deficiency of cold-inducible RNA-binding protein exacerbated monocrotaline-induced pulmonary artery hypertension through Caveolin1 and CAVIN1.
Application : IHC-P
Reactivity : Rat
El-Sherbiny M et al. Front Pharmacol 2021; 12:631216 Leflunomide Induces Dose-Dependent Lung Injury in Mice via Stimulating Vimentin and NLRP3 Inflammasome Production.
Application : IHC-P
Reactivity : Mouse
Missinato MA et al. Dev Dyn 2021; Zebrafish heart regenerates after chemoptogenetic cardiomyocyte depletion.
Reactivity : Zebrafish
Chen X et al. Biomed Pharmacother 2021; 137:111318 Qingda granule attenuates cardiac fibrosis via suppression of the TGF-β1/Smad2/3 signaling pathway in vitro and in vivo.
Application : WB
Reactivity : Rat
Hassan HM et al. Am J Cancer Res 2021; 11 (2):350-369 Adamantane-linked isothiourea derivatives suppress the growth of experimental hepatocellular carcinoma via inhibition of TLR4-MyD88-NF-κB signaling.
Application : IHC-P
Reactivity : Rat
Milani M et al. Research Square 2021; (Epub) Niclosamide Targets Inammatory and Probrotic Pathways in Amyotrophic Lateral Sclerosis.
Application : ICC/IF
Chen H.Y. et al. Cancers 2021; 13 (6):1236 Isoliquiritigenin Reverses Epithelial-Mesenchymal Transition Through Modulation of the TGF-β/Smad Signaling Pathway in Endometrial Cancer.
Application : WB
Reactivity : Human
Lee PC et al. Mol Nutr Food Res 2021; 65 (3):e2000937 Active Vitamin D3 Treatment Attenuated Bacterial Translocation via Improving Intestinal Barriers in Cirrhotic Rats.
Application : WB
Reactivity : Rat
Chang CJ et al. Biochem Pharmacol 2021; 183:114356 Overcoming interferon (IFN)-γ resistance ameliorates transforming growth factor (TGF)-β-mediated lung fibroblast-to-myofibroblast transition and bleomycin-induced pulmonary fibrosis.
Application : WB
Reactivity : Human
Chiang MT et al. Arterioscler Thromb Vasc Biol 2021; 41 (1):331-345 Gal-1 (Galectin-1) Upregulation Contributes to Abdominal Aortic Aneurysm Progression by Enhancing Vascular Inflammation.
Application : IHC-P
Reactivity : Mouse
Talia Nasr et al. Dis Model Mech 2020; Disruption of a hedgehog-foxf1-rspo2 signaling axis leads to tracheomalacia and a loss of sox9+ tracheal chondrocytes.
Application : IHC-Fr
Reactivity : Mouse
Hamada-Tsutsumi S et al. Int J Mol Sci 2020; 21 (19) Inhibitory Effect of a Human MicroRNA, miR-6133-5p, on the Fibrotic Activity of Hepatic Stellate Cells in Culture.
Yeh LC et al. Int J Mol Sci 2020; 21 (5) The Bioactive Core and Corona Synergism of Quantized Gold Enables Slowed Inflammation and Increased Tissue Regeneration in Wound Hypoxia.
Chang JF et al. Toxins (Basel) 2020; 12 (6) Therapeutic Targeting of Aristolochic Acid Induced Uremic Toxin Retention, SMAD 2/3 and JNK/ERK Pathways in Tubulointerstitial Fibrosis: Nephroprotective Role of Propolis in Chronic Kidney Disease.
Application : WB
Reactivity : Human
Rojas-Morales P et al. Free Radic Biol Med 2020; 154:75-83 Protection against renal ischemia and reperfusion injury by short-term time-restricted feeding involves the mitochondrial unfolded protein response.
Application : WB
Reactivity : Rat
Tanaka HY et al. Biomaterials 2020; 251:120077 Heterotypic 3D pancreatic cancer model with tunable proportion of fibrotic elements.
Application : WB
Reactivity : Human
Wang X et al. Toxins (Basel) 2020; 12 (4) Nephrotoxic Effects in Zebrafish after Prolonged Exposure to Aristolochic Acid.
Application : IHC-P
Reactivity : Zebrafish
Kanno Y et al. Arthritis Res Ther 2020; 22 (1):76 Alternatively Activated Macrophages Are Associated With the α2AP Production That Occurs With the Development of Dermal Fibrosis : The Role of Alternatively Activated Macrophages on the Development of Fibrosis.
Application : WB
Reactivity : Mouse
Goto Y et al. J Oral Biosci 2020; (Epub) PLAG1 enhances the stemness profiles of acinar cells in normal human salivary glands in a cell type-specific manner.
Application : IHC-Fr
Reactivity : Human
Lopez-Silva TL et al. Biomaterials 2020; 231:119667 Chemical functionality of multidomain peptide hydrogels governs early host immune response.
Application : IHC-P
Reactivity : Rat
Sun Y et al. Med Sci Monit 2019; 25:6244-6254 SOX18 Affects Cell Viability, Migration, Invasiveness, and Apoptosis in Hepatocellular Carcinoma (HCC) Cells by Participating in Epithelial-to-Mesenchymal Transition (EMT) Progression and Adenosine Monophosphate Activated Protein Kinase (AMPK)/Mammalian Target of Rapamycin (mTOR).
Application : WB
Reactivity : Human
Wang HC et al. Theranostics 2019; 9 (24):7168-7183 Pancreatic stellate cells activated by mutant KRAS-mediated PAI-1 upregulation foster pancreatic cancer progression via IL-8.
Application : ICC/IF, IHC-P
Reactivity : Human, Mouse
Lin PH et al. J Pineal Res 2019;:e12620 Melatonin activates cell death programs for the suppression of uterine leiomyoma cell proliferation.
Application : IHC-P
Reactivity : Rat
Rojas-Morales P et al. Biomolecules 2019; 9 (9) Mechanisms of Fasting-Mediated Protection against Renal Injury and Fibrosis Development after Ischemic Acute Kidney Injury.
Application : WB
Reactivity : Rat
Lixia Yang et al. European Journal of Inflammation 2019; (Epub) Effects of total flavonoids from Oxytropis falcata Bunge on the SOCS/JAK/STAT inflammatory signaling pathway in the kidneys of diabetic nephropathy model mice.
Application : WB
Reactivity : Mouse
Merlo B et al. BMC Vet Res 2019; 15 (1):277 Comparison between adult and foetal adnexa derived equine post-natal mesenchymal stem cells.
Application : ICC/IF
Lin PH et al. Cancers (Basel) 2019; 11 (8) Isoliquiritigenin Suppresses E2-Induced Uterine Leiomyoma Growth through the Modulation of Cell Death Program and the Repression of ECM Accumulation.
Application : WB, IHC-P
Reactivity : Rat
Stone OA et al. Dev Cell 2019; (Epub) Paraxial Mesoderm Is the Major Source of Lymphatic Endothelium.
Application : IHC-Fr
Reactivity : Mouse
Chen HY et al. Antioxidants (Basel) 2019; 8 (4) Natural Antioxidant Resveratrol Suppresses Uterine Fibroid Cell Growth and Extracellular Matrix Formation In Vitro and In Vivo.
Application : WB
Reactivity : Human
Noe N et al. Lab Invest 2019; (Epub) Mesenchyme-specific deletion of Tgf-β1 in the embryonic lung disrupts branching morphogenesis and induces lung hypoplasia.
Application : IHC-P
Reactivity : Mouse
Cheong ML et al. PLoS One 2019; 14 (1):e0210765 Connective tissue growth factor mediates transforming growth factor β-induced collagen expression in human endometrial stromal cells.
Application : IHC-P
Reactivity : Human
Turini S et al. Int J Mol Sci 2019; 20 (1) Epithelial to Mesenchymal Transition in Human Mesothelial Cells Exposed to Asbestos Fibers: Role of TGF-β as Mediator of Malignant Mesothelioma Development or Metastasis via EMT Event.
Application : WB
Reactivity : Human
Li HY et al. J Agric Food Chem 2019; 67 (7):1889-1901 Melatonin Inhibits in Vitro Smooth Muscle Cell Inflammation and Proliferation and Atherosclerosis in Apolipoprotein E-Deficient Mice.
Application : WB
Reactivity : Mouse
Wei LY et al. Oral Oncol 2019; 88:115-123 Reciprocal activation of cancer-associated fibroblasts and oral squamous carcinoma cells through CXCL1.
Application : IHC-P
Reactivity : Human
Tatomir A et al. Immunol Res 2018; 66 (4):445-461 RGC-32 regulates reactive astrocytosis and extracellular matrix deposition in experimental autoimmune encephalomyelitis.
Application : WB
Reactivity : Rat
Ya Wang et al. BBRC 2018; 2018:43107 Androgen receptor regulates cardiac fibrosis in mice with experimental autoimmune myocarditis by increasing microRNA-125b expression.
Application : WB
Reactivity : Mouse
Zhang Z et al. Genesis 2018;:e23256 Generation of MLC-2v-tdTomato knock-in reporter mouse line.
Srivastava SP et al. Cell Death Dis 2018; 9 (10):997 SIRT3 deficiency leads to induction of abnormal glycolysis in diabetic kidney with fibrosis.
Application : WB
Reactivity : Human
Chen HY et al. Cell Physiol Biochem 2018; 49 (5):1970-1986 Fucoidan Inhibits the Proliferation of Leiomyoma Cells and Decreases Extracellular Matrix-Associated Protein Expression.
Application : WB
Reactivity : Rat
Garcia-Arroyo FE et al. PLoS One 2018; 13 (8):e0202901 Probiotic supplements prevented oxonic acid-induced hyperuricemia and renal damage.
Application : IHC-P
Reactivity : Rat
Chen WY et al. Cell Mol Immunol 2018; (Epub) Human dendritic cell-specific ICAM-3-grabbing non-integrin downstream signaling alleviates renal fibrosis via Raf-1 activation in systemic candidiasis.
Application : IHC-Fr
Reactivity : Mouse
Fan Z et al. Cell Physiol Biochem 2018; 47 (5):1977-1988 Bushenshugan Formula Attenuates the Development of Lung Cancer by Inhibiting Epithelial-Mesenchymal Transition.
Application : WB
Reactivity : Human
Ge J et al. J Biol Chem 2018; (Epub) RhoA, Rac1 and Cdc42 differentially regulate aSMA and collagen I expression in mesenchymal stem cells.
Application : WB
Reactivity : Mouse
Liu G et al. Oncogene 2018; (Epub) Lung fibroblasts promote metastatic colonization through upregulation of stearoyl-CoA desaturase 1 in tumor cells.
Application : WB
Reactivity : Human
Feng F et al. Oncotarget 2017; 8 (3):5349-5360 Guidance to rational use of pharmaceuticals in gallbladder sarcomatoid carcinoma using patient-derived cancer cells and whole exome sequencing.
Chen PC et al. J Cell Physiol 2017; (Epub) Activation of Fibroblasts by Nicotine Promotes the Epithelial-Mesenchymal Transition and Motility of Breast Cancer Cells.
Application : WB
Reactivity : Human
Lv L et al. Exp Clin Endocrinol Diabetes 2017; (Epub) Invasive Pituitary Adenoma-Derived Tumor-Associated Fibroblasts Promote Tumor Progression both In Vitro and In Vivo.
Application : WB, ICC/IF
Reactivity : Rat
Chen CT et al. Am J Cancer Res 2017; 7 (3):657-672 A tumor vessel-targeting fusion protein elicits a chemotherapeutic bystander effect in pancreatic ductal adenocarcinoma.
Application : IHC-Fr
Reactivity : Human
Marro J et al. PLoS One 2016; 11 (10):e0165497 Collagen XII Contributes to Epicardial and Connective Tissues in the Zebrafish Heart during Ontogenesis and Regeneration.
Application : IHC-Fr
Reactivity : Zebrafish
Jheng HF et al. Mol Nutr Food Res 2016; (Epub) Dietary low-fat soy milk powder retards diabetic nephropathy progression via inhibition of renal fibrosis and renal inflammation.
Application : IHC-P
Reactivity : Mouse
Srivastava SP et al. Sci Rep 2016; 6:29884 Effect of Antifibrotic MicroRNAs Crosstalk on the Action of N-acetyl-seryl-aspartyl-lysyl-proline in Diabetes-related Kidney Fibrosis.
Reactivity : Mouse
Kim JH et al. J Tissue Eng Regen Med 2016; (Epub) Combinatorial therapy with three-dimensionally cultured adipose-derived stromal cells and self-assembling peptides to enhance angiogenesis and preserve cardiac function in infarcted hearts.
Pavan C et al. Toxicol Sci 2016; (Epub) Abrasion of Artificial Stones as a New Cause of an Ancient Disease. Physicochemical Features and Cellular Responses.
Chuang HM et al. Front Pharmacol 2016; 7:112 The Role of Butylidenephthalide in Targeting the Microenvironment Which Contributes to Liver Fibrosis Amelioration.
Application : WB, IHC-P
Reactivity : Rat
Kumar VA et al. Biomaterials 2016; 98:113-119 Treatment of hind limb ischemia using angiogenic peptide nanofibers.
Application : IHC-P
Reactivity : Mouse
Kanasaki K et al. Diabetes 2014; 63 (6):2120-31 Linagliptin-mediated DPP-4 inhibition ameliorates kidney fibrosis in streptozotocin-induced diabetic mice by inhibiting endothelial-to-mesenchymal transition in a therapeutic regimen.
Application : WB
Reactivity : Mouse
Shinohara Y et al. Eur J Histochem 2014; 58 (4):2337 Inhibition of fibrous adhesion formation in the temporomandibular joint of tenascin-C knockout mice.
Application : IHC-P
Reactivity : Mouse
McCracken KW et al. Nature 2014; Modelling human development and disease in pluripotent stem-cell-derived gastric organoids.
Reactivity : Human
Tsai TH et al. PLoS One 2014; 9 (4):e95443 Pigment Epithelium-Derived Factor 34-mer Peptide Prevents Liver Fibrosis and Hepatic Stellate Cell Activation through Down-Regulation of the PDGF Receptor.
Reactivity : Rat
Tan Q et al. J Biol Chem 2013; 288 (24):17134-44 Erythrocytosis and pulmonary hypertension in a mouse model of human HIF2A gain of function mutation.
Application : WB
Chablais F et al. Development 2012; 139 (11):1921-30 The regenerative capacity of the zebrafish heart is dependent on TGFβ signaling.
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