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alpha Smooth Muscle Actin antibody

Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034
Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034

Cat No. GTX100034

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat, Zebrafish
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

HeLa , C8D30 , NIH-3T3 , C2C12 , Rat2 , *Mouse Heart

Predict Reactivity

Rabbit, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human alpha Smooth Muscle Actin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Actin, Alpha 2, Smooth Muscle, Aorta,Actsa,Acta2

Cellular Localization

Cytoplasm , cytoskeleton

Background

The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]

Database

Research Area

DATA IMAGES
Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034

GTX100034 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034

GTX100034 WB Image

alpha smooth muscle Actin antibody detects alpha smooth muscle Actin protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha smooth muscle Actin antibody (GTX100034) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034

GTX100034 IHC-P Image

alpha smooth muscle Actin antibody detects alpha smooth muscle Actin protein at cytoplasm on human liver carcinoma stroma by immunohistochemical analysis.
Sample: Paraffin-embedded liver carcinoma stroma.
alpha smooth muscle Actin antibody (GTX100034) d
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034

GTX100034 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034

GTX100034 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using alpha Actin (smooth muscle)(GTX100034) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Smooth Muscle Actin antibody used in IHC (Paraffin sections) (IHC-P). GTX100034

GTX100034 IHC-P Image

alpha smooth muscle Actin antibody detects alpha smooth muscle Actin protein at cytosol on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
alpha smooth muscle Actin antibody (GTX100034) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Smooth Muscle Actin antibody used in Western Blot (WB). GTX100034

GTX100034 WB Image

alpha smooth muscle Actin antibody detects ACTA2 protein by western blot analysis.
A. 30 µg Rat2 whole cell lysate/extract
10% SDS-PAGE
alpha smooth muscle Actin antibody (GTX100034) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

alpha Smooth Muscle Actin antibody Cat No. GTX100034

Rating ( Average 4 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4 based on 2 users reviews)
Date : Anonymous submitted on 10-Aug-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : Pancreatic cancer associated fibroblasts
Amount used : 20 μg
Blocking : 5% skim milk in TBST, 25°C, 2Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Lane Description : Lane 1: Control group, Lane 2: Treated with a compound which decreases the expression of a-SMA at a transcriptional level.
Date : Anonymous submitted on 04-May-2012
Score :
Application Tested : WB
Sample Species : Ms
Sample : Mouse heart tssue
Amount used : 20μg
Blocking : 5% milk, 25°C, 2Hr
Primary Antibody : 1:2000, 4°C, overnightHr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169