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alpha Synuclein antibody [4B12]

Anti-alpha Synuclein antibody [4B12] used in Immunohistochemistry (IHC). GTX21904

Cat No. GTX21904

Host

Mouse

Clonality

Monoclonal

Clone Name

4B12

Isotype

IgG1

Application

WB, IHC-P, IHC-Fr, Dot, ELISA, IHC

Reactivity

Human, Mouse
Package
100 μl ($339)

APPLICATION

Application Note

Dot: Use at a dilution of 1/100 - 1/10000. ELISA: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1/100 - 1/1000. Antigen retrieval is not essential but may optimise staining. IHC-Fr: Use at a dilution of 1/100 - 1/1000. WB: Use at a dilution of 1/100 - 1/10000. Predicted molecular weight: 16 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

14 kDa. ( Note )

Product Note

We do not recommend use of this product for Mouse,Rat.

PROPERTIES

Form

Liquid

Buffer

Preservative: 0.1% Sodium Azide; Constituents: PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human alpha synuclein produced in E. coli.

Purification


Ammonium sulfate precipitated and dialyzed tissue culture supernatant.

Conjugation

Unconjugated

RRID

AB_380314

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

synuclein alpha , NACP , PARK1 , PARK4 , PD1

Cellular Localization

Cytoplasm , Membrane , Nucleus , Cell junction, synapse , Secreted

Background

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-alpha Synuclein antibody [4B12] used in Immunohistochemistry (IHC). GTX21904

GTX21904 IHC Image

IHC analysis of the cortical Lewy body using alpha Synuclein antibody [4B12].

REFERENCE

REVIEW

alpha Synuclein antibody [4B12] Cat No. GTX21904

Rating ( Average 5 based on 4 users reviews)
Application
Western Blot(WB) ( Average 5 based on 4 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Acta Neuropathol Commun in 2018.PMID: 29298733
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 29184069
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Mol Neurodegener in 2017.PMID: 28676095
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2017.PMID: 27845893
Application Reference
SDS
PBS.pdf
Sodium Azide.pdf