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alpha Synuclein antibody [4B12]

Orthogonal
Anti-alpha Synuclein antibody [4B12] used in Immunohistochemistry (IHC). GTX21904
Anti-alpha Synuclein antibody [4B12] used in Western Blot (WB). GTX21904
Anti-alpha Synuclein antibody [4B12] used in Western Blot (WB). GTX21904
Anti-alpha Synuclein antibody [4B12] used in Western Blot (WB). GTX21904
Anti-alpha Synuclein antibody [4B12] used in Western Blot (WB). GTX21904

Cat. No. GTX21904

Reference 10

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4B12

Isotype

 IgG1

Application

 WB, IHC-P, IHC-Fr, Dot, ELISA, IHC

Reactivity

 Human
Package
100 μl ($399)
See all alpha Synuclein products

APPLICATION

Application Note

Dot: Use at a dilution of 1/100 - 1/10000. ELISA: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1/100 - 1/1000. Antigen retrieval is not essential but may optimise staining. IHC-Fr: Use at a dilution of 1/100 - 1/1000. WB: Use at a dilution of 1/100 - 1/10000. Predicted molecular weight: 16 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

14 kDa. ( Note )

Product Note

We do not recommend use of this product for Mouse,Rat samples.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human alpha synuclein produced in E. coli.

Purification


Ammonium sulfate precipitated and dialyzed tissue culture supernatant.

Conjugation

Unconjugated

RRID

AB_380314

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

synuclein alpha , NACP , PARK1 , PARK4 , PD1

Cellular Localization

Cytoplasm , Membrane , Nucleus , Cell junction, synapse , Secreted

Background

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-alpha Synuclein antibody [4B12] used in Immunohistochemistry (IHC). GTX21904

GTX21904 IHC Image

IHC analysis of the cortical Lewy body using alpha Synuclein antibody [4B12].

Anti-alpha Synuclein antibody [4B12] used in Western Blot (WB). GTX21904

GTX21904 WB Image

Orthogonal

The data was published in the journal Acta Neuropathol Commun in 2018.PMID: 29298733

Anti-alpha Synuclein antibody [4B12] used in Western Blot (WB). GTX21904

GTX21904 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2017. PMID: 27845893

Anti-alpha Synuclein antibody [4B12] used in Western Blot (WB). GTX21904

GTX21904 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2017. PMID: 29184069

Anti-alpha Synuclein antibody [4B12] used in Western Blot (WB). GTX21904

GTX21904 WB Image

Orthogonal

The data was published in the journal Mol Neurodegener in 2017. PMID: 28676095

REFERENCE

Application Reference

Guo Q et al. CNS Neurosci Ther 2023; 29 (6) : 1547-1560 α-Synuclein decoy peptide protects mice against α-synuclein-induced memory loss.
Cheng A et al. Acta Pharmacol Sin 2022; 43 (3) : 552-562 Fatty acid-binding protein 7 triggers α-synuclein oligomerization in glial cells and oligodendrocytes associated with oxidative stress.
Lemos M et al. Transl Neurodegener 2020; 9 (1) : 38 Targeting α-synuclein by PD03 AFFITOPE? and Anle138b rescues neurodegenerative pathology in a model of multiple system atrophy: clinical relevance.
Application : WB
Heras-Garvin A et al. J Neuroinflammation 2020; 17 (1) : 35 High-salt diet does not boost neuroinflammation and neurodegeneration in a model of α-synucleinopathy.
Application : Dot
Reactivity : Human
Refolo V et al. Acta Neuropathol Commun 2018; 6 (1) : 2 Progressive striatonigral degeneration-in a transgenic mouse model of multiple system atrophy: translational implications for interventional therapies.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Guo Q et al. CNS Neurosci Ther 2023; 29 (6):1547-1560 α-Synuclein decoy peptide protects mice against α-synuclein-induced memory loss.
Cheng A et al. Acta Pharmacol Sin 2022; 43 (3):552-562 Fatty acid-binding protein 7 triggers α-synuclein oligomerization in glial cells and oligodendrocytes associated with oxidative stress.
Lemos M et al. Transl Neurodegener 2020; 9 (1):38 Targeting α-synuclein by PD03 AFFITOPE? and Anle138b rescues neurodegenerative pathology in a model of multiple system atrophy: clinical relevance.
Application : WB
Heras-Garvin A et al. J Neuroinflammation 2020; 17 (1):35 High-salt diet does not boost neuroinflammation and neurodegeneration in a model of α-synucleinopathy.
Application : Dot
Reactivity : Human
Refolo V et al. Acta Neuropathol Commun 2018; 6 (1):2 Progressive striatonigral degeneration-in a transgenic mouse model of multiple system atrophy: translational implications for interventional therapies.
Application : WB
Reactivity : Human
Karampetsou M et al. Sci Rep 2017; 7 (1):16533 Phosphorylated exogenous alpha-synuclein fibrils exacerbate pathology and induce neuronal dysfunction in mice.
Application : WB
Reactivity : Human
Venezia S et al. Mol Neurodegener 2017; 12 (1):52 Toll-like receptor 4 stimulation with monophosphoryl lipid A ameliorates motor deficits and nigral neurodegeneration triggered by extraneuronal α-synucleinopathy.
Application : WB
Reactivity : Human
Pampalakis G et al. Oncotarget 2016; (Epub) KLK6 proteolysis is implicated in the turnover and uptake of extracellular alpha-synuclein species.
Application : WB
Reactivity : Human
Emmanouilidou E et al. Brain 2016; 139 (Pt 3):871-90 GABA transmission via ATP-dependent K+ channels regulates α-synuclein secretion in mouse striatum.
Application : WB
Helwig M et al. Brain 2016; 139 (Pt 3):856-70 Brain propagation of transduced α-synuclein involves non-fibrillar protein species and is enhanced in α-synuclein null mice.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399