alpha Synuclein antibody [HL1243]

Cat. No. GTX636642

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1243
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all alpha Synuclein products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	Assay dependent
IHC-P	Assay dependent
IHC-Fr	Assay dependent

Not tested in other applications.

Predict Reactivity

Sheep, Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human alpha Synuclein Recombinant protein.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910023

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Database

Gene ID: 6622

Research Area

DATA IMAGES

GTX636642 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with alpha Synuclein antibody [HL1243] (GTX636642) diluted at 1:10000 and competitor's antibody (Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX636642 IHC-P Image

alpha Synuclein antibody [HL1243] detects alpha Synuclein protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
alpha Synuclein stained by alpha Synuclein antibody [HL1243] (GTX636642) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636642 ICC/IF Image

alpha Synuclein antibody [HL1243] detects alpha Synuclein protein by immunofluorescent analysis.
Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Synuclein stained by alpha Synuclein antibody [HL1243] (GTX636642) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

GTX636642 IHC-P Image

alpha Synuclein antibody [HL1243] detects alpha Synuclein protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Green: alpha Synuclein stained by alpha Synuclein antibody [HL1243] (GTX636642) diluted at 1:100.
Red: NF-H, a neural marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636642 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody [HL1243] (GTX636642) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636642 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody [HL1243] (GTX636642) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX636642 IHC-Fr Image

alpha Synuclein antibody [HL1242] detects alpha Synuclein protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: alpha Synuclein stained by alpha Synuclein antibody [HL1242] (GTX636642) diluted at 1:100.

GTX636642 IHC-Fr Image

alpha Synuclein antibody [HL1243] detects alpha Synuclein protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: alpha Synuclein stained by alpha Synuclein antibody [HL1243] (GTX636642) diluted at 1:100.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

alpha Synuclein antibody [HL1243]

Cat. No. GTX636642

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Database

Research Area

DATA IMAGES

GTX636642 WB Image

GTX636642 IHC-P Image

GTX636642 ICC/IF Image

GTX636642 IHC-P Image

GTX636642 WB Image

GTX636642 WB Image

GTX636642 IHC-Fr Image

GTX636642 IHC-Fr Image

REFERENCE

REVIEW

Product

Research

Support

Company

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