alpha Synuclein antibody [HL1243]

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with alpha Synuclein antibody [HL1243] (GTX636642) diluted at 1:10000 and competitor's antibody (Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, Human Alpha Synuclein protein, His tag (GTX112799-pro) (50 ng). Coated protein was probed with alpha Synuclein antibodies (3.81*10^-5-160 nM). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.
1. alpha Synuclein antibody [HL1242] (GTX636641)
2. alpha Synuclein antibody [HL1243] (GTX636642)

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody [HL1243] (GTX636642) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

alpha Synuclein antibody [HL1243] detects alpha Synuclein protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Green: alpha Synuclein stained by alpha Synuclein antibody [HL1243] (GTX636642) diluted at 1:100.
Red: NF-H, a neural marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody [HL1243] (GTX636642) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

alpha Synuclein antibody [HL1243] detects alpha Synuclein protein by immunofluorescent analysis.
Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Synuclein stained by alpha Synuclein antibody [HL1243] (GTX636642) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

alpha Synuclein antibody [HL1243] detects alpha Synuclein protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
alpha Synuclein stained by alpha Synuclein antibody [HL1243] (GTX636642) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

alpha Synuclein antibody [HL1243] detects alpha Synuclein protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: alpha Synuclein stained by alpha Synuclein antibody [HL1243] (GTX636642) diluted at 1:100.

alpha Synuclein antibody [HL1242] detects alpha Synuclein protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: alpha Synuclein stained by alpha Synuclein antibody [HL1242] (GTX636642) diluted at 1:100.