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alpha Synuclein antibody

Anti-alpha Synuclein antibody used in Western Blot (WB). GTX133367
Anti-alpha Synuclein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133367
Anti-alpha Synuclein antibody used in IHC (Frozen sections) (IHC-Fr). GTX133367
Anti-alpha Synuclein antibody used in Western Blot (WB). GTX133367
Anti-alpha Synuclein antibody used in IHC (Frozen sections) (IHC-Fr). GTX133367

Cat No. GTX133367

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain

Predict Reactivity

Sheep, Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human alpha Synuclein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886957

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

synuclein alpha , NACP , PARK1 , PARK4 , PD1

Cellular Localization

Cytoplasm , Membrane , Nucleus

Background

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Four alternatively spliced transcripts encoding two different isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-alpha Synuclein antibody used in Western Blot (WB). GTX133367

GTX133367 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with alpha Synuclein antibody (GTX133367) diluted at 1:2000 and competitor's antibody (MO22112) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-alpha Synuclein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133367

GTX133367 ICC/IF Image

alpha Synuclein antibody detects alpha Synuclein protein by immunofluorescent analysis.
Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX133367) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-alpha Synuclein antibody used in IHC (Frozen sections) (IHC-Fr). GTX133367

GTX133367 IHC-Fr Image

alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX133367) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-alpha Synuclein antibody used in Western Blot (WB). GTX133367

GTX133367 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with alpha Synuclein antibody (GTX133367) diluted at 1:1000.

Anti-alpha Synuclein antibody used in IHC (Frozen sections) (IHC-Fr). GTX133367

GTX133367 IHC-Fr Image

alpha Synuclein antibody detects alpha Synuclein protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse hippocampus.
Green: alpha Synuclein protein stained by alpha Synuclein antibody (GTX133367) diluted at 1:250.
Red: NeuN, stained by NeuN antibody [2Q158] (GTX30773) diluted at 1:500.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169