APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:2000 |
| 1:100-1:500 |
| 1:50-1:200 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rabbit, Bovine, Dog, Chicken, Pig, Horse(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human Synuclein alpha around the phosphorylation site of Ser129.
Purification
Purified by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns
From serum
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
synuclein alpha , NACP , PARK1 , PARK4 , PD1
Cellular Localization
Cytoplasm , Membrane , Nucleus , Cell junction, synapse , Secreted
Background
Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016]
Database
Research Area
DATA IMAGES
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GTX02537 IHC-P Image
IHC-P analysis of human gastric cancer tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:200
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GTX02537 IHC-P Image
IHC-P analysis of rat brain tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:200
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GTX02537 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:200
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GTX02537 IHC-P Image
IHC-P analysis of rat brain tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX02537 ELISA Image
ELISA analysis of phospho- and non phosho- immunogen peptide using GTX02537 alpha Synuclein (phospho Ser129) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml
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GTX02537 IHC-P Image
IHC-P analysis of rat kidney tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:200
|
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GTX02537 IHC-P Image
IHC-P analysis of human lung cancer tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:200
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GTX02537 IHC-P Image
IHC-P analysis of mouse liver tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:200
|
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GTX02537 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:200
|
|
|
GTX02537 IHC-P Image
IHC-P analysis of human kidney tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:200
|
|
|
GTX02537 IHC-P Image
IHC-P analysis of human lung cancer tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:200
|
|
|
GTX02537 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
|
|
|
GTX02537 IHC-P Image
IHC-P analysis of human esophagus tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:200
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GTX02537 ICC/IF Image
ICC/IF analysis of PFA-fixed H₂O₂-treated HeLa cells using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Red : Primary antibody
Green : Tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100
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GTX02537 IHC-P Image
IHC-P analysis of human gastric cancer tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:200
|
|
|
GTX02537 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:200
|
|
|
GTX02537 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
|
|
|
GTX02537 IHC-P Image
IHC-P analysis of rat kidney tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:200
|
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|
GTX02537 WB Image
WB analysis of extracts from rat and mouse brain tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
N peptide : Non-phospho peptide
P peptide : Phospho peptide
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REFERENCE
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REVIEW
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