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alpha Synuclein (phospho Ser129) antibody

Anti-alpha Synuclein (phospho Ser129) antibody used in Western Blot (WB). GTX02537
Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537
Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537
Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537
Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537
Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537
Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537
Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537
Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537
Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537
Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537
Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537
Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537
Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537
Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537
Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537
Anti-alpha Synuclein (phospho Ser129) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02537
Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537
Anti-alpha Synuclein (phospho Ser129) antibody used in ELISA (ELISA). GTX02537

Cat No. GTX02537

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
ELISA Assay dependent
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Pig, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Synuclein alpha around the phosphorylation site of Ser129.

Purification

Purified by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

synuclein alpha , NACP , PARK1 , PARK4 , PD1

Cellular Localization

Cytoplasm , Membrane , Nucleus , Cell junction, synapse , Secreted

Background

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-alpha Synuclein (phospho Ser129) antibody used in Western Blot (WB). GTX02537

GTX02537 WB Image

WB analysis of extracts from rat and mouse brain tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
N peptide : Non-phospho peptide
P peptide : Phospho peptide

Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537

GTX02537 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:200

Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537

GTX02537 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:200

Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537

GTX02537 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:200

Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537

GTX02537 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:200

Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537

GTX02537 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:200

Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537

GTX02537 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:200

Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537

GTX02537 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:200

Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537

GTX02537 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:200

Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537

GTX02537 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537

GTX02537 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:200

Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537

GTX02537 IHC-P Image

IHC-P analysis of human kidney tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:200

Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537

GTX02537 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537

GTX02537 IHC-P Image

IHC-P analysis of human esophagus tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:200

Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537

GTX02537 IHC-P Image

IHC-P analysis of rat brain tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537

GTX02537 IHC-P Image

IHC-P analysis of rat brain tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:200

Anti-alpha Synuclein (phospho Ser129) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02537

GTX02537 ICC/IF Image

ICC/IF analysis of PFA-fixed H₂O₂-treated HeLa cells using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Red : Primary antibody
Green : Tubulin
Blue : DAPI
Permeabilization : 0.1% Triton X-100

Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX02537

GTX02537 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX02537 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:200

Anti-alpha Synuclein (phospho Ser129) antibody used in ELISA (ELISA). GTX02537

GTX02537 ELISA Image

ELISA analysis of phospho- and non phosho- immunogen peptide using GTX02537 alpha Synuclein (phospho Ser129) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml

REFERENCE

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REVIEW

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