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alpha Synuclein (phospho Ser129) antibody

Anti-alpha Synuclein (phospho Ser129) antibody used in Western Blot (WB). GTX50222
Anti-alpha Synuclein (phospho Ser129) antibody used in Western Blot (WB). GTX50222
Anti-alpha Synuclein (phospho Ser129) antibody used in Western Blot (WB). GTX50222

Cat No. GTX50222

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 129 (M-P-S(p)-E-E) derived from human alpha Synuclein.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

RRID

AB_11179523

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

synuclein alpha , NACP , PARK1 , PARK4 , PD1

Cellular Localization

Cytoplasm,Membrane,Nucleus,Cell junction, synapse,Secreted

Background

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-alpha Synuclein (phospho Ser129) antibody used in Western Blot (WB). GTX50222

GTX50222 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein (phospho Ser129) antibody (GTX50222) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Synuclein (phospho Ser129) antibody used in Western Blot (WB). GTX50222

GTX50222 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with alpha Synuclein (phospho Ser129) antibody (GTX50222) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Synuclein (phospho Ser129) antibody used in Western Blot (WB). GTX50222

GTX50222 WB Image

WB analysis of extracts from mouse brain tissue using GTX50222 alpha Synuclein (phospho Ser129) antibody.
Lane 1 : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

REVIEW

alpha Synuclein (phospho Ser129) antibody Cat No. GTX50222

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2019.PMID: 30787269
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2019.PMID: 30787269
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf