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alpha Synuclein (phospho Ser129) antibody

Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX54991
Anti-alpha Synuclein (phospho Ser129) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54991
Anti-alpha Synuclein (phospho Ser129) antibody used in Western Blot (WB). GTX54991

Cat No. GTX54991

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of alpha Synuclein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887909

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

synuclein alpha , NACP , PARK1 , PARK4 , PD1

Cellular Localization

Cytoplasm,Membrane,Nucleus,Cell junction, synapse,Secreted

Background

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-alpha Synuclein (phospho Ser129) antibody used in IHC (Paraffin sections) (IHC-P). GTX54991

GTX54991 IHC-P Image

IHC-P analysis of formalin fixed human prostate cancer tissue section using GTX54991 alpha Synuclein (phospho Ser129) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-alpha Synuclein (phospho Ser129) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54991

GTX54991 ICC/IF Image

ICC/IF analysis of formalin-fixed HEK293T cells using GTX54991 alpha Synuclein (phospho Ser129) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-alpha Synuclein (phospho Ser129) antibody used in Western Blot (WB). GTX54991

GTX54991 WB Image

WB analysis of HEK293T (A), mouse brain (B) whole cell lysates using GTX54991 alpha Synuclein (phospho Ser129) antibody.

REFERENCE

REVIEW

alpha Synuclein (phospho Ser129) antibody Cat No. GTX54991

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Front Pharmacol in 2020.PMID: 32180729
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Front Pharmacol in 2020.PMID: 32180729
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Front Pharmacol in 2020.PMID: 32180729
SDS
Glycerol.pdf
Sodium Azide.pdf