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alpha TTP antibody [C2C3], C-term

Anti-alpha TTP antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108286
Anti-alpha TTP antibody [C2C3], C-term used in Western Blot (WB). GTX108286
Anti-alpha TTP antibody [C2C3], C-term used in Western Blot (WB). GTX108286
Anti-alpha TTP antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108286
Anti-alpha TTP antibody [C2C3], C-term used in Western Blot (WB). GTX108286

Cat. No. GTX108286

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Hamster
Package
100 μl ($399),
25 μl ($169)
See all alpha TTP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

IMR32 , mouse liver , rat liver

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human alpha TTP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038188

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

alpha tocopherol transfer protein , ATTP , AVED , TTP1 , alphaTTP

Cellular Localization

Cytoplasm

Background

This gene encodes a soluble protein that binds alpha-trocopherol, a form of vitamin E, with high selectivity and affinity. This protein plays an important role in regulating vitamin E levels in the body by transporting vitamin E between membrane vesicles and facilitating the secretion of vitamin E from hepatocytes to circulating lipoproteins. Mutations in this gene cause hereditary vitamin E deficiency (ataxia with vitamin E deficiency, AVED) and retinitis pigmentosa. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-alpha TTP antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108286

GTX108286 ICC/IF Image

Immunofluorescence analysis of methanol-fixed MCF-7, using alpha TTP(GTX108286) antibody at 1:500 dilution.

Anti-alpha TTP antibody [C2C3], C-term used in Western Blot (WB). GTX108286

GTX108286 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with alpha TTP antibody [C2C3], C-term (GTX108286) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha TTP antibody [C2C3], C-term used in Western Blot (WB). GTX108286

GTX108286 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with alpha TTP antibody [C2C3], C-term (GTX108286) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha TTP antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108286

GTX108286 IHC-P Image

alpha TTP antibody [C2C3], C-term detects TTPA protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
alpha TTP antibody [C2C3], C-term (GTX108286) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha TTP antibody [C2C3], C-term used in Western Blot (WB). GTX108286

GTX108286 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
12% SDS PAGE
GTX108286 diluted at 1:1000

REFERENCE

Application Reference

Aeschimann W et al. Sci Rep 2017; 7 (1) : 4970 Self-assembled α-Tocopherol Transfer Protein Nanoparticles Promote Vitamin E Delivery Across an Endothelial Barrier.
Application : WB
Reactivity : Human
Grebenstein N et al. Mol Nutr Food Res 2014; α-Tocopherol transfer protein is not required for the discrimination against γ-tocopherol in vivo but protects it from side-chain degradation in vitro.
Application : WB
Reactivity : Mouse
Laothong U et al. Parasitol Int 2013; 62 (2) : 127-33 α-Tocopherol and lipid profiles in plasma and the expression of α-tocopherol-related molecules in the liver of Opisthorchis viverrini-infected hamsters.
Application : WB
Reactivity : Hamster
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169