GeneTex
United States (US)

alpha TTP antibody [C2C3], C-term

GTX108286_39750_IFA_19011507_176.jpg
GTX108286_39750_20180119_WB_R_liver_19011507_766.jpg
GTX108286_39750_IHC_19011507_590.jpg
GTX108286_39750_20180119_WB_M_liver_19011507_882.jpg
GTX108286_WB_19011507_593.jpg

Cat No. GTX108286

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat, Hamster
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

IMR32 , mouse liver , rat liver
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human alpha TTP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Alpha Tocopherol Transfer Protein,Attp,Aved,Ttp1,Alphattp,Ttpa

Cellular Localization

Cytoplasm

Background

This gene encodes a soluble protein that binds alpha-trocopherol, a form of vitamin E, with high selectivity and affinity. This protein plays an important role in regulating vitamin E levels in the body by transporting vitamin E between membrane vesicles and facilitating the secretion of vitamin E from hepatocytes to circulating lipoproteins. Mutations in this gene cause hereditary vitamin E deficiency (ataxia with vitamin E deficiency, AVED) and retinitis pigmentosa. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108286_39750_IFA_19011507_176.jpg

GTX108286 ICC/IF Image

Immunofluorescence analysis of methanol-fixed MCF-7, using alpha TTP(GTX108286) antibody at 1:500 dilution.

GTX108286_39750_20180119_WB_R_liver_19011507_766.jpg

GTX108286 WB Image

Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with alpha TTP antibody [C2C3], C-term (GTX108286) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108286_39750_IHC_19011507_590.jpg

GTX108286 IHC-P Image

alpha TTP antibody [C2C3], C-term detects TTPA protein at cytosol on human gastric cancer by immunohistochemical analysis.
Sample: Paraffin-embedded gastric cancer.
alpha TTP antibody [C2C3], C-term (GTX108286) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108286_39750_20180119_WB_M_liver_19011507_882.jpg

GTX108286 WB Image

Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with alpha TTP antibody [C2C3], C-term (GTX108286) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108286_WB_19011507_593.jpg

GTX108286 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
12% SDS PAGE
GTX108286 diluted at 1:1000

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169