APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , Jurkat , Raji , NIH-3T3
Predict Reactivity
Rat, Zebrafish, Bovine, Chicken, Pig, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human alpha Tubulin 1A. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10621168
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tubulin alpha 1a , B-ALPHA-1 , LIS3 , TUBA3
Background
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX115048 IHC-P Image
alpha Tubulin 1A antibody detects alpha Tubulin 1A protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
alpha Tubulin 1A antibody (GTX115048) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX115048 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using alpha Tubulin 1A(GTX115048) antibody at 1:200 dilution.
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GTX115048 WB Image
Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX115048 diluted at 1:1000
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GTX115048 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human breast cancer, using alpha Tubulin 1A(GTX115048) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX115048 IHC-P Image
alpha Tubulin 1A antibody detects alpha Tubulin 1A protein at cytosol on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
alpha Tubulin 1A antibody (GTX115048) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX115048 WB Image
Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: JurKat
D: Raji
10% SDS PAGE
GTX115048 diluted at 1:1000
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GTX115048 WB Image
alpha Tubulin 1A antibody detects alpha Tubulin 1A protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10 % SDS-PAGE, and blotted with alpha Tubulin 1A antibody (GTX115048) diluted by 1:1000
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REFERENCE
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REVIEW
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