GeneTex
United States (US)

alpha Tubulin 1A antibody

GTX115048_40247_IHC_18121410_409.jpg
GTX115048_40247_WB_M_18121410_904.jpg
GTX115048_WB_18121410_654.jpg
GTX115048_41059_IHC_M_2_18121410_984.jpg
GTX115048_41059_IHC_M_18121410_636.jpg
GTX115048_IFA_18121410_767.jpg

Cat No. GTX115048

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji , NIH-3T3

Predict Reactivity

Rat, Zebrafish, Bovine, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin 1A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tubulin Alpha 1A,B-Alpha-1,Lis3,Tuba3,Tuba1A

Background

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX115048_40247_IHC_18121410_409.jpg

GTX115048 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using alpha Tubulin 1A(GTX115048) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115048_40247_WB_M_18121410_904.jpg

GTX115048 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX115048 diluted at 1:1000

GTX115048_WB_18121410_654.jpg

GTX115048 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: JurKat
D: Raji
10% SDS PAGE
GTX115048 diluted at 1:1000

GTX115048_41059_IHC_M_2_18121410_984.jpg

GTX115048 IHC-P Image

alpha Tubulin 1A antibody detects alpha Tubulin 1A protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
alpha Tubulin 1A antibody (GTX115048) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115048_41059_IHC_M_18121410_636.jpg

GTX115048 IHC-P Image

alpha Tubulin 1A antibody detects alpha Tubulin 1A protein at cytosol on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
alpha Tubulin 1A antibody (GTX115048) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115048_IFA_18121410_767.jpg

GTX115048 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using alpha Tubulin 1A(GTX115048) antibody at 1:200 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169