alpha Tubulin 8 antibody [2G6]

Name: alpha Tubulin 8 antibody [2G6]
Brand: GeneTex
SKU: GTX83462
Price: 519.00 USD

Anti-alpha Tubulin 8 antibody [2G6] used in IHC (Paraffin sections) (IHC-P). GTX83462

Anti-alpha Tubulin 8 antibody [2G6] used in Immunoprecipitation (IP). GTX83462

Anti-alpha Tubulin 8 antibody [2G6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83462

Anti-alpha Tubulin 8 antibody [2G6] used in Western Blot (WB). GTX83462

Cat. No. GTX83462

Host	Mouse
Clonality	Monoclonal
Clone Name	2G6
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Rat, Dog, Monkey
Package	100 μl ($519)

See all alpha Tubulin 8 products

Datasheet
Document

View product citations for antibody GTX83462 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:2000
ICC/IF	1:100
IHC-P	1:50-1:150
IP	Assay dependent

Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.82 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human TUBA8(NP_061816) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tubulin alpha 8 , CDCBM8 , TUBAL2

Cellular Localization

Cytoplasm, cytoskeleton

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Database

Gene ID: 51807 TUBA8
UniProt: Q9NY65 TUBA8

Research Area

DATA IMAGES

GTX83462 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX83462 alpha Tubulin 8 antibody [2G6].

GTX83462 IHC-P Image

IHC-P analysis of kidney tissue using GTX83462 alpha Tubulin 8 antibody [2G6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83462 IP Image

IP analysis of DDDDK tagged alpha Tubulin 8 overexpressed HEK293T lysate using GTX83462 alpha Tubulin 8 antibody [2G6].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

GTX83462 IHC-P Image

IHC-P analysis of prostate tissue using GTX83462 alpha Tubulin 8 antibody [2G6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83462 IHC-P Image

IHC-P analysis of pancreas tissue using GTX83462 alpha Tubulin 8 antibody [2G6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83462 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with alpha Tubulin 8 plasmid using GTX83462 alpha Tubulin 8 antibody [2G6].

GTX83462 IHC-P Image

IHC-P analysis of human endometrium adenocarcinoma tissue using GTX83462 alpha Tubulin 8 antibody [2G6].

GTX83462 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX83462 alpha Tubulin 8 antibody [2G6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX83462 WB Image

WB analysis of various cell lines using GTX83462 alpha Tubulin 8 antibody [2G6].
Loading : 35 ug per lane

GTX83462 WB Image

WB analysis of HEK293T cells transfected with alpha Tubulin 8 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83462 alpha Tubulin 8 antibody [2G6].
Loading : 5 ug per lane

GTX83462 IHC-P Image

IHC-P analysis of colon adenocarcinoma tissue using GTX83462 alpha Tubulin 8 antibody [2G6].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

alpha Tubulin 8 antibody [2G6]

Cat. No. GTX83462

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX83462 IHC-P Image

GTX83462 IHC-P Image

GTX83462 IP Image

GTX83462 IHC-P Image

GTX83462 IHC-P Image

GTX83462 ICC/IF Image

GTX83462 IHC-P Image

GTX83462 IHC-P Image

GTX83462 WB Image

GTX83462 WB Image

GTX83462 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.