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alpha Tubulin 8 antibody [N1C1-2]

Anti-alpha Tubulin 8 antibody [N1C1-2] used in Western Blot (WB). GTX110432
Anti-alpha Tubulin 8 antibody [N1C1-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110432
Anti-alpha Tubulin 8 antibody [N1C1-2] used in IHC (Paraffin sections) (IHC-P). GTX110432
Anti-alpha Tubulin 8 antibody [N1C1-2] used in Western Blot (WB). GTX110432

Cat No. GTX110432

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3

Predict Reactivity

Rat, Bovine, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin 8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tubulin Alpha 8,Cdcbm8,Tubal2,Tuba8

Background

This gene encodes a member of the alpha tubulin protein family. Alpha tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Mutations in this gene are associated with polymicrogyria and optic nerve hypoplasia. Alternate splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-alpha Tubulin 8 antibody [N1C1-2] used in Western Blot (WB). GTX110432

GTX110432 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX110432 diluted at 1:5000

Anti-alpha Tubulin 8 antibody [N1C1-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110432

GTX110432 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using alpha Tubulin 8(GTX110432) antibody at 1:200 dilution.

Anti-alpha Tubulin 8 antibody [N1C1-2] used in IHC (Paraffin sections) (IHC-P). GTX110432

GTX110432 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using alpha Tubulin 8(GTX110432) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Tubulin 8 antibody [N1C1-2] used in Western Blot (WB). GTX110432

GTX110432 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: Hela
10% SDS PAGE
GTX110432 diluted at 1:1000

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169