GeneTex

  • Country / Location Selection

United States (US)

alpha Tubulin 8 antibody [N1C1-2]

Anti-alpha Tubulin 8 antibody [N1C1-2] used in IHC (Paraffin sections) (IHC-P). GTX110432
Anti-alpha Tubulin 8 antibody [N1C1-2] used in Western Blot (WB). GTX110432
Anti-alpha Tubulin 8 antibody [N1C1-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110432
Anti-alpha Tubulin 8 antibody [N1C1-2] used in Western Blot (WB). GTX110432
Anti-alpha Tubulin 8 antibody [N1C1-2] used in Western Blot (WB). GTX110432

Cat. No. GTX110432

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all alpha Tubulin 8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

NIH-3T3 , A431 , HeLa

Predict Reactivity

Rat, Bovine, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin 8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952432

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tubulin alpha 8 , CDCBM8 , TUBAL2

Background

This gene encodes a member of the alpha tubulin protein family. Alpha tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Mutations in this gene are associated with polymicrogyria and optic nerve hypoplasia. Alternate splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-alpha Tubulin 8 antibody [N1C1-2] used in IHC (Paraffin sections) (IHC-P). GTX110432

GTX110432 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using alpha Tubulin 8(GTX110432) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Tubulin 8 antibody [N1C1-2] used in Western Blot (WB). GTX110432

GTX110432 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX110432 diluted at 1:5000

Anti-alpha Tubulin 8 antibody [N1C1-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110432

GTX110432 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using alpha Tubulin 8(GTX110432) antibody at 1:200 dilution.

Anti-alpha Tubulin 8 antibody [N1C1-2] used in Western Blot (WB). GTX110432

GTX110432 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: Hela
10% SDS PAGE
GTX110432 diluted at 1:1000

Anti-alpha Tubulin 8 antibody [N1C1-2] used in Western Blot (WB). GTX110432

GTX110432 WB Image

The data was published in the journal Genome Biol in 2019. PMID: 30791937

REFERENCE

Application Reference

Sharma S et al. Genome Biol 2019; 20 (1) : 37 Mitochondrial hypoxic stress induces widespread RNA editing by APOBEC3G in natural killer cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for alpha Tubulin 8 antibody [N1C1-2] (GTX110432). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169