alpha Tubulin antibody [236-10501]

WB analysis of decreasing amounts of a-tubulin protein using GTX31126 alpha Tubulin antibody [236-10501].

ICC/IF analysis of HeLa cells using GTX31126 alpha Tubulin antibody [236-10501].
Magenta : Primary antibody
Purple : Actin
Blue : DNA

ICC/IF analysis of bovine pulmonary artery endothelial cells using GTX31126 alpha Tubulin antibody [236-10501].

ICC/IF analysis of bovine pulmonary artery endothelial (BPAE) cells using GTX31126 alpha Tubulin antibody [236-10501].
Blue : Primary antibody
Red : Actin
Green : wheat germ agglutinin to stain components of endosomal pathways

IHC-Fr analysis of 14 μm coronal section of mouse hippocampus using GTX31126 alpha Tubulin antibody [236-10501].
Green : Primary antibody
Blue : cell nucleus
Red : neuronal cell bodies

ICC/IF analysis of HeLa cells using GTX31126 alpha Tubulin antibody [236-10501]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1 μg/ml in 1% BSA and incubated for 3 hours at room temperature

ICC/IF analysis of bovine pulmonary artery endothelial (BPAE) cells using GTX31126 alpha Tubulin antibody [236-10501].
Green : Actin
Red : Alpha tubulin

IHC-P analysis of human astroglioma tissue using GTX31126 alpha Tubulin antibody [236-10501].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

ICC/IF analysis of NIH-3T3 cells using GTX31126 alpha Tubulin antibody [236-10501].
Blue : Primary antibody
Red : cell nucleus

ICC/IF analysis of permeabilized muntjac skin fibroblasts using GTX31126 alpha Tubulin antibody [236-10501].
Green :alpha tubulin
Yellow : Paxillin
Blue : CDC6

ICC/IF analysis of bovine pulmonary endothelial cells using GTX31126 alpha Tubulin antibody [236-10501].
Blue : Alpha tubulin
Green : Nuclei
Red : mitochondrion tracker
Fixation : formaldehyde
Permeabilization : Acetone

WB analysis of 20 μg of A431 (Lane 1), Raji (Lane 2), MDA-MB-231 (Lane 3), HCT 116 (Lane 4), HeLa (Lane 5), DU 145 (Lane 6), PC-3 (Lane 7) and Caco-2 (Lane 8) cell lysate using GTX31126 alpha Tubulin antibody [236-10501].
Dilution : 1:4000