GeneTex
United States (US)

alpha Tubulin antibody [236-10501]

GTX31126_748_ICC-IF_19011519_755.jpg
GTX31126_981_IHC_19011519_117.jpg
GTX31126_1338_IHC-P_19011519_940.jpg
GTX31126_739_ICC-IF_19011519_502.jpg
GTX31126_735_ICC-IF_19011519_729.jpg
GTX31126_744_ICC-IF_19011519_886.jpg
GTX31126_2083_WB_19011519_871.jpg
GTX31126_754_ICC-IF_19011519_837.jpg
GTX31126_737_ICC-IF_19011519_644.jpg
GTX31126_743_ICC-IF_19011519_447.jpg
GTX31126_2011_ICC-IF_19011519_561.jpg
GTX31126_1848_WB_19011519_753.jpg

Cat No. GTX31126

Host Mouse
Clonality Monoclonal
Clone Name 236-10501
Isotype IgG1
Application WB, ICC/IF, IHC-P, IHC-Fr, FACS, IHC
Reactivity Human, Mouse, Rat, Drosophila, Bovine, Hamster, Marsupials, Primate
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:5000
ICC/IF 1:100-1:500
IHC-P 1:20-1:200
IHC-Fr Assay dependent
FACS Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )
PROPERTIES

Form

Liquid

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Bovine

Immunogen

Bovine Alpha-Tubulin.

Purification

Purified IgG

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Cellular Localization

Cytoplasm,Cytoskeleton

Research Area

DATA IMAGES
GTX31126_748_ICC-IF_19011519_755.jpg

GTX31126 ICC/IF Image

ICC/IF analysis of bovine pulmonary artery endothelial (BPAE) cells using GTX31126 alpha Tubulin antibody [236-10501].
Blue : Primary antibody
Red : Actin
Green : wheat germ agglutinin to stain components of endosomal pathways

GTX31126_981_IHC_19011519_117.jpg

GTX31126 IHC-Fr Image

IHC-Fr analysis of 14 µm coronal section of mouse hippocampus using GTX31126 alpha Tubulin antibody [236-10501].
Green : Primary antibody
Blue : cell nucleus
Red : neuronal cell bodies

GTX31126_1338_IHC-P_19011519_940.jpg

GTX31126 IHC-P Image

IHC-P analysis of human astroglioma tissue using GTX31126 alpha Tubulin antibody [236-10501].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

GTX31126_739_ICC-IF_19011519_502.jpg

GTX31126 ICC/IF Image

ICC/IF analysis of bovine pulmonary endothelial cells using GTX31126 alpha Tubulin antibody [236-10501].
Blue : Alpha tubulin
Green : Nuclei
Red : mitochondrion tracker
Fixation : formaldehyde
Permeabilization : Acetone

GTX31126_735_ICC-IF_19011519_729.jpg

GTX31126 ICC/IF Image

ICC/IF analysis of bovine pulmonary artery endothelial cells using GTX31126 alpha Tubulin antibody [236-10501].

GTX31126_744_ICC-IF_19011519_886.jpg

GTX31126 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX31126 alpha Tubulin antibody [236-10501].
Blue : Primary antibody
Red : cell nucleus

GTX31126_2083_WB_19011519_871.jpg

GTX31126 WB Image

WB analysis of decreasing amounts of a-tubulin protein using GTX31126 alpha Tubulin antibody [236-10501].

GTX31126_754_ICC-IF_19011519_837.jpg

GTX31126 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31126 alpha Tubulin antibody [236-10501].
Magenta : Primary antibody
Purple : Actin
Blue : DNA

GTX31126_737_ICC-IF_19011519_644.jpg

GTX31126 ICC/IF Image

ICC/IF analysis of bovine pulmonary artery endothelial (BPAE) cells using GTX31126 alpha Tubulin antibody [236-10501].
Green : Actin
Red : Alpha tubulin

GTX31126_743_ICC-IF_19011519_447.jpg

GTX31126 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31126 alpha Tubulin antibody [236-10501]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1 µg/ml in 1% BSA and incubated for 3 hours at room temperature

GTX31126_2011_ICC-IF_19011519_561.jpg

GTX31126 ICC/IF Image

ICC/IF analysis of permeabilized muntjac skin fibroblasts using GTX31126 alpha Tubulin antibody [236-10501].
Green :alpha tubulin
Yellow : Paxillin
Blue : CDC6

GTX31126_1848_WB_19011519_753.jpg

GTX31126 WB Image

WB analysis of 20 µg of A431 (Lane 1), Raji (Lane 2), MDA-MB-231 (Lane 3), HCT 116 (Lane 4), HeLa (Lane 5), DU 145 (Lane 6), PC-3 (Lane 7) and Caco-2 (Lane 8) cell lysate using GTX31126 alpha Tubulin antibody [236-10501].
Dilution : 1:4000

Package List Price ($)
$ 319