alpha Tubulin antibody [236-10501]

Name: alpha Tubulin antibody [236-10501]
Brand: GeneTex
SKU: GTX31126
Price: 399.00 USD

Anti-alpha Tubulin antibody [236-10501] used in Western Blot (WB). GTX31126

Anti-alpha Tubulin antibody [236-10501] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31126

Anti-alpha Tubulin antibody [236-10501] used in IHC (Paraffin sections) (IHC-P). GTX31126

Anti-alpha Tubulin antibody [236-10501] used in IHC (Frozen sections) (IHC-Fr). GTX31126

Cat. No. GTX31126

Host	Mouse
Clonality	Monoclonal
Clone Name	236-10501
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS, IHC
Reactivity	Human, Mouse, Rat, Drosophila, Bovine, Hamster, Marsupials, Primate
Package	50 μg ($399)

See all alpha Tubulin products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:5000
ICC/IF	1:100-1:500
IHC-P	1:20-1:200
IHC-Fr	Assay dependent
FACS	Assay dependent
IHC	Assay dependent

Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

Bovine Alpha-Tubulin.

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tubulin alpha 4a

Cellular Localization

Cytoplasm, cytoskeleton

Database

Gene ID: 777775 TUBA4A
UniProt: P81948 TUBA4A

Research Area

DATA IMAGES

GTX31126 WB Image

WB analysis of 20 μg of A431 (Lane 1), Raji (Lane 2), MDA-MB-231 (Lane 3), HCT 116 (Lane 4), HeLa (Lane 5), DU 145 (Lane 6), PC-3 (Lane 7) and Caco-2 (Lane 8) cell lysate using GTX31126 alpha Tubulin antibody [236-10501].
Dilution : 1:4000

GTX31126 ICC/IF Image

ICC/IF analysis of bovine pulmonary endothelial cells using GTX31126 alpha Tubulin antibody [236-10501].
Blue : Alpha tubulin
Green : Nuclei
Red : mitochondrion tracker
Fixation : formaldehyde
Permeabilization : Acetone

GTX31126 ICC/IF Image

ICC/IF analysis of permeabilized muntjac skin fibroblasts using GTX31126 alpha Tubulin antibody [236-10501].
Green :alpha tubulin
Yellow : Paxillin
Blue : CDC6

GTX31126 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX31126 alpha Tubulin antibody [236-10501].
Blue : Primary antibody
Red : cell nucleus

GTX31126 IHC-P Image

IHC-P analysis of human astroglioma tissue using GTX31126 alpha Tubulin antibody [236-10501].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

GTX31126 ICC/IF Image

ICC/IF analysis of bovine pulmonary artery endothelial (BPAE) cells using GTX31126 alpha Tubulin antibody [236-10501].
Green : Actin
Red : Alpha tubulin

GTX31126 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31126 alpha Tubulin antibody [236-10501]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1 μg/ml in 1% BSA and incubated for 3 hours at room temperature

GTX31126 IHC-Fr Image

IHC-Fr analysis of 14 μm coronal section of mouse hippocampus using GTX31126 alpha Tubulin antibody [236-10501].
Green : Primary antibody
Blue : cell nucleus
Red : neuronal cell bodies

GTX31126 ICC/IF Image

ICC/IF analysis of bovine pulmonary artery endothelial (BPAE) cells using GTX31126 alpha Tubulin antibody [236-10501].
Blue : Primary antibody
Red : Actin
Green : wheat germ agglutinin to stain components of endosomal pathways

GTX31126 ICC/IF Image

ICC/IF analysis of bovine pulmonary artery endothelial cells using GTX31126 alpha Tubulin antibody [236-10501].

GTX31126 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX31126 alpha Tubulin antibody [236-10501].
Magenta : Primary antibody
Purple : Actin
Blue : DNA

GTX31126 WB Image

WB analysis of decreasing amounts of a-tubulin protein using GTX31126 alpha Tubulin antibody [236-10501].

REFERENCE

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REVIEW

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Package

List Price ($)

50 μg

$ 399

Qty

alpha Tubulin antibody [236-10501]

Cat. No. GTX31126

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Database

Research Area

DATA IMAGES

GTX31126 WB Image

GTX31126 ICC/IF Image

GTX31126 ICC/IF Image

GTX31126 ICC/IF Image

GTX31126 IHC-P Image

GTX31126 ICC/IF Image

GTX31126 ICC/IF Image

GTX31126 IHC-Fr Image

GTX31126 ICC/IF Image

GTX31126 ICC/IF Image

GTX31126 ICC/IF Image

GTX31126 WB Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.