alpha Tubulin antibody [B-5-1-2]

Name: alpha Tubulin antibody [B-5-1-2]
Brand: GeneTex
SKU: GTX11304
Price: 419.00 USD
Rating: 5 (2 reviews)

Anti-alpha Tubulin antibody [B-5-1-2] used in Western Blot (WB). GTX11304

Cat. No. GTX11304

Reference 7

Host	Mouse
Clonality	Monoclonal
Clone Name	B-5-1-2
Isotype	IgG1
Application	WB, ICC/IF, IP
Reactivity	Human, Mouse, Rat, Zebrafish, Bovine, Dog, Chicken, Monkey, African green monkey, Kangaroo rat, Sea Urchin, Chlamydomonas
Package	100 μl ($419)

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APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	0.25-0.5 μg/ml
ICC/IF	0.5-1 μg/ml
IP	Assay dependent

Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

chicken fibroblast, FS11

Product Note

Recognizes an epitope located at the C-terminal end of the α-tubulin isoform in a variety of organisms.

PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

~2? mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Sea Urchin

Immunogen

Sarkosyl-resistant filaments from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.

Purification

Purified immunoglobulin

Conjugation

Unconjugated

RRID

AB_373242

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tubulin alpha 4a , ALS22 , H2-ALPHA , TUBA1

Cellular Localization

Cytoplasm, cytoskeleton

Background

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.

Database

Gene ID: 7277 TUBA4A
UniProt: P68366 TUBA4A

Research Area

DATA IMAGES

GTX11304 WB Image

WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH-3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK and (9) MDCK lysates using alpha Tubulin antibody [B-5-1-2] at 0.5 μg/ml.

REFERENCE

Application Reference

Auer TO et al. Elife 2015; 4 (Epub) Deletion of a kinesin I motor unmasks a mechanism of homeostatic branching control by neurotrophin-3.

Application : WB

Reactivity : Zebrafish

De Domenico I et al. Cell Metab 2011; 14 (5) : 635-46 The role of ubiquitination in hepcidin-independent and hepcidin-dependent degradation of ferroportin.

Huang AM et al. Biochem Pharmacol 2011; 82 (1) : 35-42 ube2m-mediated p27(kip1) degradation in * cytotoxicity.

Application : WB

Reactivity : Human

De Domenico I et al. Proc Natl Acad Sci U S A 2009; 106 (10) : 694083 Hepcidin-induced internalization of ferroportin requires binding and cooperative interaction with Jak2.

Application : WB

Reactivity : Human

Paradkar PN et al. Mol Cell Biol 2009; 29 (4) : 1007-16 Regulation of mitochondrial iron import through differential turnover of mitoferrin 1 and mitoferrin 2.

Application : WB

Reactivity : Mouse

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