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alpha Tubulin antibody [DM1A]

Anti-alpha Tubulin antibody [DM1A] used in Western Blot (WB). GTX11302
Anti-alpha Tubulin antibody [DM1A] used in IHC (Paraffin sections) (IHC-P). GTX11302

Cat No. GTX11302

Host Mouse
Clonality Monoclonal
Clone Name DM1A
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat, Yeast, Bovine, Dog, Chicken, Monkey, Fungi
Package
100 μl ($329)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
ICC/IF 1:500
IHC-P Assay dependent
Not tested in other applications.

Positive Control

chicken fibroblast

Specificity/Sensitivity

The antibody is specific for α-tubulin in immunoblotting assays and may be used for localization of α-tubulin in cultured cells or tissue sections. The antibody reacts best with chicken fibroblasts.
PROPERTIES

Form

Liquid

Buffer

Ascites, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Chicken

Immunogen

microtubules from chicken embryo brain.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

TUBA1, P68366, alpha Tubulin, 191110, H2ALPHA, H2-ALPHA, 7277, FLJ30169, TUBA1B, TUBA2, TUBA3D, TUBA8, H2 ALPHA

Background

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha-tubulin and beta-tubulin; both subunits have a molecular weight of approximately 50 kDa and share considerable homology. The most widely studied tubulins have been isolated from vertebrate brains. The microtubules can be viewed in immunofluorescent microscopy, which enables the observation of the intracellular organization of proteins that are in the form of a supramolecular structure.GT211302 may be used to study the intracellular organization and distribution of tubulin along the static and dynamic aspects of the cytoskeleton. The product can be used for the immunocytochemical localization of the alpha chain of tubulin by means of indirect immunofluorescent labeling of cultured cells, formalin fixed or frozen tissues or for specific identification of the alpha-tubulin band in immunoblotting or immunoprecipitation techniques.

Research Area

DATA IMAGES
Anti-alpha Tubulin antibody [DM1A] used in Western Blot (WB). GTX11302

GTX11302 WB Image

WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH-3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK and (9) MDCK lysates using alpha Tubulin antibody [DM1A] at a dilution of 1:500.

Anti-alpha Tubulin antibody [DM1A] used in IHC (Paraffin sections) (IHC-P). GTX11302

GTX11302 IHC-P Image

IHC-P analysis of human heart tissue using GTX11302 alpha Tubulin antibody [DM1A] at 1:500.

REFERENCE
REVIEW
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Package List Price ($)
$ 329