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alpha Tubulin antibody [DM1A]

Anti-alpha Tubulin antibody [DM1A] used in Western Blot (WB). GTX11302
Anti-alpha Tubulin antibody [DM1A] used in IHC (Paraffin sections) (IHC-P). GTX11302

Cat. No. GTX11302

Reference 21
4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 DM1A

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Yeast, Bovine, Dog, Chicken, Monkey, Fungi
Package
100 μl ($509)
See all alpha Tubulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 1:500
IHC-P Assay dependent
Not tested in other applications.

Positive Control

chicken fibroblast

Product Note

The antibody is specific for α-tubulin in immunoblotting assays and may be used for localization of α-tubulin in cultured cells or tissue sections. The antibody reacts best with chicken fibroblasts.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Chicken

Immunogen

microtubules from chicken embryo brain.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_381291

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tubulin alpha 8a , TUBA4A , TUBA8

Background

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha-tubulin and beta-tubulin; both subunits have a molecular weight of approximately 50 kDa and share considerable homology. The most widely studied tubulins have been isolated from vertebrate brains. The microtubules can be viewed in immunofluorescent microscopy, which enables the observation of the intracellular organization of proteins that are in the form of a supramolecular structure.GT211302 may be used to study the intracellular organization and distribution of tubulin along the static and dynamic aspects of the cytoskeleton. The product can be used for the immunocytochemical localization of the alpha chain of tubulin by means of indirect immunofluorescent labeling of cultured cells, formalin fixed or frozen tissues or for specific identification of the alpha-tubulin band in immunoblotting or immunoprecipitation techniques.

Database

Research Area

DATA IMAGES

Anti-alpha Tubulin antibody [DM1A] used in Western Blot (WB). GTX11302

GTX11302 WB Image

WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH-3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK and (9) MDCK lysates using alpha Tubulin antibody [DM1A] at a dilution of 1:500.

Anti-alpha Tubulin antibody [DM1A] used in IHC (Paraffin sections) (IHC-P). GTX11302

GTX11302 IHC-P Image

IHC-P analysis of human heart tissue using GTX11302 alpha Tubulin antibody [DM1A] at 1:500.

REFERENCE

Application Reference

Yang CC et al. Cell Rep 2022; 38 (10) : 110472 CRM1-spike-mediated nuclear export of hepatitis B virus encapsidated viral RNA.
Hsieh YL et al. Cell Rep 2019; 29 (8) : 2134-2143.e7 NPGPx-Mediated Adaptation to Oxidative Stress Protects Motor Neurons from Degeneration in Aging by Directly Modulating O-GlcNAcase.
Application : WB
Reactivity : Mouse
Rathore AP et al. J Clin Invest 2019; 130 Dengue virus-elicited tryptase induces endothelial permeability and shock.
Application : ICC/IF
Reactivity : Human

Clone Reference

Young AM et al. Mol Biol Cell 2020; 31 (15):1551-1560 BAF facilitates interphase nuclear membrane repair through recruitment of nuclear transmembrane proteins.
Galmozzi A et al. Nature 2019; 576 (7785):138-142 PGRMC2 is an intracellular haem chaperone critical for adipocyte function.
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Submit a Reference

REVIEW

alpha Tubulin antibody [DM1A] Cat. No. GTX11302

Rating
( Average 5 based on 4 users reviews)
Application
Western Blot(WB)
( Average 5 based on 4 users reviews)
Anti-alpha Tubulin antibody [DM1A] used in Western Blot (WB). GTX11302
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal EMBO Mol Med in 2013. PMID: 23828861
Anti-alpha Tubulin antibody [DM1A] used in Western Blot (WB). GTX11302
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal EMBO Mol Med in 2013. PMID: 23828861
Anti-alpha Tubulin antibody [DM1A] used in Western Blot (WB). GTX11302
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal EMBO Mol Med in 2013. PMID: 23828861
Anti-alpha Tubulin antibody [DM1A] used in Western Blot (WB). GTX11302
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Genome Med in 2012. PMID: 23270647
Submit a Review
Application Reference
Yang CC et al. Cell Rep 2022; 38 (10):110472 CRM1-spike-mediated nuclear export of hepatitis B virus encapsidated viral RNA.
Hsieh YL et al. Cell Rep 2019; 29 (8):2134-2143.e7 NPGPx-Mediated Adaptation to Oxidative Stress Protects Motor Neurons from Degeneration in Aging by Directly Modulating O-GlcNAcase.
Application : WB
Reactivity : Mouse
Rathore AP et al. J Clin Invest 2019; 130 Dengue virus-elicited tryptase induces endothelial permeability and shock.
Application : ICC/IF
Reactivity : Human
Chou SF et al. PLoS Pathog 2015; 11 (10):e1005123 The Dual Role of an ESCRT-0 Component HGS in HBV Transcription and Naked Capsid Secretion.
Application : WB
Reactivity : Human
Chang YC et al. EMBO Mol Med 2013; 5 (8):1165-79 Deficiency of NPGPx, an oxidative stress sensor, leads to obesity in mice and human.
Application : WB
Reactivity : Mouse
Wei PC et al. Nucleic Acids Res 2013; 41 (3):1533-43 A cis-element with mixed G-quadruplex structure of NPGPx promoter is essential for nucleolin-mediated transactivation on non-targeting siRNA stress.
Application : WB
Reactivity : Human
Raymond J Louie et al. Genome Med. 2012; 4 (12) A yeast phenomic model for the gene interaction network modulating CFTR-ΔF508 protein biogenesis
Application : WB
Reactivity : Human
Wei PC et al. Mol Cell 2012; 48 (5):747-59 Loss of the oxidative stress sensor NPGPx compromises GRP78 chaperone activity and induces systemic disease.
Application : WB
Reactivity : Mouse
Wei PC et al. Nucleic Acids Res 2012; 40 (1):323-32 Non-targeting siRNA induces NPGPx expression to cooperate with exoribonuclease XRN2 for releasing the stress.
Application : WB
Wang D et al. PLoS One 2011; 6 (5):e19629 Downregulation of FIP200 induces apoptosis of glioblastoma cells and microvascular endothelial cells by enhancing Pyk2 activity.
Application : WB
Reactivity : Human
Li HC et al. PLoS Pathog 2010; 6 (10):e1001162 Nuclear export and import of human hepatitis B virus capsid protein and particles.
Application : ICC/IF
Reactivity : Human
Clone Reference
Young AM et al. Mol Biol Cell 2020; 31 (15):1551-1560 BAF facilitates interphase nuclear membrane repair through recruitment of nuclear transmembrane proteins.
Galmozzi A et al. Nature 2019; 576 (7785):138-142 PGRMC2 is an intracellular haem chaperone critical for adipocyte function.
Lee PC et al. Cancer Cell 2018; 34 (6):954-969.e4 Targeting PKCδ as a Therapeutic Strategy against Heterogeneous Mechanisms of EGFR Inhibitor Resistance in EGFR-Mutant Lung Cancer.
Wu T et al. Proc Natl Acad Sci U S A 2017; 114 (45):11956-11961 HSPB7 is indispensable for heart development by modulating actin filament assembly.
Azimzadeh O et al. Int J Radiat Biol 2017;:43109 Proteome analysis of irradiated endothelial cells reveals persistent alteration in protein degradation and the RhoGDI and NO signalling pathways.
Huang CK et al. Nat Commun 2017; 8:14706 Adipocytes promote malignant growth of breast tumours with monocarboxylate transporter 2 expression via β-hydroxybutyrate.
Casciati A et al. Oncotarget 2016; 7 (19):28040-58 Age-related effects of X-ray irradiation on mouse hippocampus.
Barjaktarovic Z et al. Int J Radiat Biol 2016;:43127 Low-dose radiation differentially regulates protein acetylation and histone deacetylase expression in human coronary artery endothelial cells.
Lee PT et al. Nucleic Acids Res 2014; Morphine drives internal ribosome entry site-mediated hnRNP K translation in neurons through opioid receptor-dependent signaling.
Bakshi MV et al. J Proteome Res 2014; Total Body Exposure to Low-Dose Ionizing Radiation Induces Long-Term Alterations to the Liver Proteome of Neonatally Exposed Mice.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 509