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alpha Tubulin antibody [DM1A]

Anti-alpha Tubulin antibody [DM1A] used in Western Blot (WB). GTX27291
Anti-alpha Tubulin antibody [DM1A] used in Western Blot (WB). GTX27291

Cat. No. GTX27291

Reference 23

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 DM1A

Isotype

 IgG1

Application

 WB, ICC/IF, IP, IHC

Reactivity

 Human, Mouse, Rat, Yeast, Bovine, Dog, Chicken, Monkey, Fungi
Package
100 μg ($439)
See all alpha Tubulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5-1 μg/ml
ICC/IF 0.5-1 μg/ml
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Positive Control

chicken fibroblast, chicken gizzard

Product Note

The antibody is specific for α-tubulin in immunoblotting assays and may be used for localization of α-tubulin in cultured cells or tissue sections. The antibody reacts best with chicken fibroblasts.

PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4, 1% BSA

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

~1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Chicken

Immunogen

microtubules from chicken embryo brain.

Purification

Purified immunoglobulin

Conjugation

Unconjugated

RRID

AB_2887584

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha-tubulin and beta-tubulin; both subunits have a molecular weight of approximately 50 kDa and share considerable homology. The most widely studied tubulins have been isolated from vertebrate brains. The microtubules can be viewed in immunofluorescent microscopy, which enables the observation of the intracellular organization of proteins that are in the form of a supramolecular structure.

Research Area

DATA IMAGES

Anti-alpha Tubulin antibody [DM1A] used in Western Blot (WB). GTX27291

GTX27291 WB Image

WB analysis of chicken gizzard extract using GTX27291 alpha Tubulin antibody [DM1A] at 1 μg/mL.

Anti-alpha Tubulin antibody [DM1A] used in Western Blot (WB). GTX27291

GTX27291 WB Image

WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH-3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK and (9) MDCK lysates using alpha Tubulin antibody [DM1A] at 1 μg/ml.

REFERENCE

Application Reference

Lee PC et al. Am J Cancer Res 2023; 13 (10) : 4693-4707 Development of triazole-based PKC-inhibitors to overcome resistance to EGFR inhibitors in EGFR-mutant lung cancers.
Hong X et al. J Biol Chem 2023; 299 (6) : 104814 Diarylheptanoid 35d overcomes EGFR TKI resistance by inducing hsp70-mediated lysosomal degradation of EGFR in EGFR-mutant lung adenocarcinoma.
Young AM et al. Mol Biol Cell 2020; 31 (15) : 1551-1560 BAF facilitates interphase nuclear membrane repair through recruitment of nuclear transmembrane proteins.
Application : WB
Reactivity : Human

Clone Reference

Yang CC et al. Cell Rep 2022; 38 (10):110472 CRM1-spike-mediated nuclear export of hepatitis B virus encapsidated viral RNA.
Rathore AP et al. J Clin Invest 2019; 130 Dengue virus-elicited tryptase induces endothelial permeability and shock.
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REVIEW

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Application Reference
Lee PC et al. Am J Cancer Res 2023; 13 (10):4693-4707 Development of triazole-based PKC-inhibitors to overcome resistance to EGFR inhibitors in EGFR-mutant lung cancers.
Hong X et al. J Biol Chem 2023; 299 (6):104814 Diarylheptanoid 35d overcomes EGFR TKI resistance by inducing hsp70-mediated lysosomal degradation of EGFR in EGFR-mutant lung adenocarcinoma.
Young AM et al. Mol Biol Cell 2020; 31 (15):1551-1560 BAF facilitates interphase nuclear membrane repair through recruitment of nuclear transmembrane proteins.
Application : WB
Reactivity : Human
Galmozzi A et al. Nature 2019; 576 (7785):138-142 PGRMC2 is an intracellular haem chaperone critical for adipocyte function.
Application : WB
Lee PC et al. Cancer Cell 2018; 34 (6):954-969.e4 Targeting PKCδ as a Therapeutic Strategy against Heterogeneous Mechanisms of EGFR Inhibitor Resistance in EGFR-Mutant Lung Cancer.
Application : WB
Reactivity : Human
Wu T et al. Proc Natl Acad Sci U S A 2017; 114 (45):11956-11961 HSPB7 is indispensable for heart development by modulating actin filament assembly.
Application : WB
Reactivity : Mouse
Clone Reference
Yang CC et al. Cell Rep 2022; 38 (10):110472 CRM1-spike-mediated nuclear export of hepatitis B virus encapsidated viral RNA.
Hsieh YL et al. Cell Rep 2019; 29 (8):2134-2143.e7 NPGPx-Mediated Adaptation to Oxidative Stress Protects Motor Neurons from Degeneration in Aging by Directly Modulating O-GlcNAcase.
Rathore AP et al. J Clin Invest 2019; 130 Dengue virus-elicited tryptase induces endothelial permeability and shock.
Azimzadeh O et al. Int J Radiat Biol 2017;:43109 Proteome analysis of irradiated endothelial cells reveals persistent alteration in protein degradation and the RhoGDI and NO signalling pathways.
Huang CK et al. Nat Commun 2017; 8:14706 Adipocytes promote malignant growth of breast tumours with monocarboxylate transporter 2 expression via β-hydroxybutyrate.
Casciati A et al. Oncotarget 2016; 7 (19):28040-58 Age-related effects of X-ray irradiation on mouse hippocampus.
Barjaktarovic Z et al. Int J Radiat Biol 2016;:43127 Low-dose radiation differentially regulates protein acetylation and histone deacetylase expression in human coronary artery endothelial cells.
Chou SF et al. PLoS Pathog 2015; 11 (10):e1005123 The Dual Role of an ESCRT-0 Component HGS in HBV Transcription and Naked Capsid Secretion.
Lee PT et al. Nucleic Acids Res 2014; Morphine drives internal ribosome entry site-mediated hnRNP K translation in neurons through opioid receptor-dependent signaling.
Bakshi MV et al. J Proteome Res 2014; Total Body Exposure to Low-Dose Ionizing Radiation Induces Long-Term Alterations to the Liver Proteome of Neonatally Exposed Mice.
Chang YC et al. EMBO Mol Med 2013; 5 (8):1165-79 Deficiency of NPGPx, an oxidative stress sensor, leads to obesity in mice and human.
Wei PC et al. Nucleic Acids Res 2013; 41 (3):1533-43 A cis-element with mixed G-quadruplex structure of NPGPx promoter is essential for nucleolin-mediated transactivation on non-targeting siRNA stress.
Wei PC et al. Mol Cell 2012; 48 (5):747-59 Loss of the oxidative stress sensor NPGPx compromises GRP78 chaperone activity and induces systemic disease.
Wei PC et al. Nucleic Acids Res 2012; 40 (1):323-32 Non-targeting siRNA induces NPGPx expression to cooperate with exoribonuclease XRN2 for releasing the stress.
Louie RJ et al. Genome Med 2012; 4 (12):103 A yeast phenomic model for the gene interaction network modulating CFTR-[increment]F508 protein biogenesis.
Wang D et al. PLoS One 2011; 6 (5):e19629 Downregulation of FIP200 induces apoptosis of glioblastoma cells and microvascular endothelial cells by enhancing Pyk2 activity.
Li HC et al. PLoS Pathog 2010; 6 (10):e1001162 Nuclear export and import of human hepatitis B virus capsid protein and particles.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 439