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alpha Tubulin antibody [DM1A]

Anti-alpha Tubulin antibody [DM1A] used in Western Blot (WB). GTX27291
Anti-alpha Tubulin antibody [DM1A] used in Western Blot (WB). GTX27291

Cat No. GTX27291

Host

Mouse

Clonality

Monoclonal

Clone Name

DM1A

Isotype

IgG1

Application

WB, ICC/IF, IP, IHC

Reactivity

Human, Mouse, Rat, Yeast, Bovine, Dog, Chicken, Monkey, Fungi
Package
100 μg ($409)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1 μg/ml
ICC/IF 0.5-1 μg/ml
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Positive Control

chicken fibroblast, chicken gizzard

Product Note

The antibody is specific for α-tubulin in immunoblotting assays and may be used for localization of α-tubulin in cultured cells or tissue sections. The antibody reacts best with chicken fibroblasts.

PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4, 1% BSA

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

~1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Chicken

Immunogen

microtubules from chicken embryo brain.

Purification

Purified immunoglobulin

Conjugation

Unconjugated

RRID

AB_2887584

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha-tubulin and beta-tubulin; both subunits have a molecular weight of approximately 50 kDa and share considerable homology. The most widely studied tubulins have been isolated from vertebrate brains. The microtubules can be viewed in immunofluorescent microscopy, which enables the observation of the intracellular organization of proteins that are in the form of a supramolecular structure.

Research Area

DATA IMAGES

Anti-alpha Tubulin antibody [DM1A] used in Western Blot (WB). GTX27291

GTX27291 WB Image

WB analysis of chicken gizzard extract using GTX27291 alpha Tubulin antibody [DM1A] at 1 μg/mL.

Anti-alpha Tubulin antibody [DM1A] used in Western Blot (WB). GTX27291

GTX27291 WB Image

WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH-3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK and (9) MDCK lysates using alpha Tubulin antibody [DM1A] at 1 μg/ml.

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409