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alpha Tubulin antibody [F2C]

Anti-alpha Tubulin antibody [F2C] used in  (). GTX33912
Anti-alpha Tubulin antibody [F2C] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33912

Cat No. GTX33912

Host

Human

Clonality

Monoclonal

Clone Name

F2C

Isotype

IgG2

Application

WB, ICC/IF

Reactivity

Human, Mouse, Bovine
Package
100 μg ($349)

APPLICATION

Application Note

Immunocytochemistry: 1:1000, Western Blot: 1:1000

Specificity/Sensitivity

Recognizes mouse, bovine and human α-tubulin.

PROPERTIES

Form

Liquid

Buffer

PBS containing 10% glycerol and 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

Bovine brain tubulin.

Purification

Protein A-affinity purified.

Purity

≧95% (SDS-PAGE)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-alpha Tubulin antibody [F2C] used in  (). GTX33912

GTX33912 Image

10μg of GTX109135-pro Galectin 2 recombinant protein analyzed using SDS-PAGE and stained with coomassie blue and captured by black and white camera.

Anti-alpha Tubulin antibody [F2C] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33912

GTX33912 ICC/IF Image

Human alpha-Tubulin is detected by immunocytochemistry using anti-alpha-Tubulin, mAb (rec.) (F2C).Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with anti-alpha-Tubulin, mAb (rec.) (F2C) (1μg /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.

REFERENCE

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REVIEW

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