GeneTex
United States (US)

alpha Tubulin antibody [F2C]

GTX33912_20160831_Structure_18121410_621.jpg
GTX33912_20160831_ICCIF_18121410_153.jpg

Cat No. GTX33912

Host Human
Clonality Monoclonal
Clone Name F2C
Isotype IgG2
Application WB, ICC/IF
Reactivity Human, Mouse, Bovine
APPLICATION

Application Note

Immunocytochemistry: 1:1000, Western Blot: 1:1000

Specificity/Sensitivity

Recognizes mouse, bovine and human α-tubulin.
PROPERTIES

Form

Liquid

Buffer

PBS containing 10% glycerol and 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

Bovine brain tubulin.

Purification

Protein A-affinity purified.

Purity

≧95% (SDS-PAGE)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX33912_20160831_Structure_18121410_621.jpg

GTX33912 Image

Structure of the recombinant antibody anti-alpha-Tubulin, mAb (rec.) (F2C). The single chain variable human fragment (ScFv) selected by antibody phage display technology and specific to the antigen of interest is fused to a human IgG2 Fc region.

GTX33912_20160831_ICCIF_18121410_153.jpg

GTX33912 ICC/IF Image

Human alpha-Tubulin is detected by immunocytochemistry using anti-alpha-Tubulin, mAb (rec.) (F2C).Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with anti-alpha-Tubulin, mAb (rec.) (F2C) (1μg /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.

Package List Price ($)
$ 349