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alpha Tubulin antibody [F2C]

Anti-alpha Tubulin antibody [F2C] used in (). GTX33912
Anti-alpha Tubulin antibody [F2C] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33912

Cat. No. GTX33912

Host

 Human

Clonality

 Monoclonal

Clone Name

 F2C

Isotype

 IgG2

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Bovine
Package
100 μg ($409)
See all alpha Tubulin products

APPLICATION

Application Note

Immunocytochemistry: 1:1000, Western Blot: 1:1000

Product Note

Recognizes mouse, bovine and human α-tubulin.

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

Bovine brain tubulin.

Purification

Protein A-affinity purified.

Purity

??95% by SDS-PAGE.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-alpha Tubulin antibody [F2C] used in (). GTX33912

GTX33912 Image

Anti-alpha Tubulin antibody [F2C] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33912

GTX33912 ICC/IF Image

Human alpha-Tubulin is detected by immunocytochemistry using anti-alpha-Tubulin, mAb (rec.) (F2C).Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with anti-alpha-Tubulin, mAb (rec.) (F2C) (1μg /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409