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alpha Tubulin antibody [GT114] (HRP)

Anti-alpha Tubulin antibody [GT114] (HRP) used in Western Blot (WB). GTX628802-01
Anti-alpha Tubulin antibody [GT114] (HRP) used in Western Blot (WB). GTX628802-01
Anti-alpha Tubulin antibody [GT114] (HRP) used in Western Blot (WB). GTX628802-01
Anti-alpha Tubulin antibody [GT114] (HRP) used in Western Blot (WB). GTX628802-01

Cat. No. GTX628802-01

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT114

Isotype

 IgG2a

Application

 WB

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all alpha Tubulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929

PROPERTIES

Form

Liquid

Buffer

0.05M Tris (pH7.4), 0.15M NaCl, 1%BSA

Preservative

0.005% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_2888054

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

K-ALPHA-1 antibody , TUBA1B antibody , alpha-tubulin ubiquitous antibody , alpha tubulin antibody , tubulin K-alpha-1 antibody , tubulin alpha-ubiquitous chain antibody , tubulin , alpha , ubiquitous antibody , tubulin alpha-1B chain antibody , tubulin , alpha 1b antibody

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Research Area

DATA IMAGES

Anti-alpha Tubulin antibody [GT114] (HRP) used in Western Blot (WB). GTX628802-01

GTX628802-01 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (HRP) (GTX628802-01) diluted at 1:1000. The HRP-conjugated anti-FALSE was used to detect the primary antibody.

Anti-alpha Tubulin antibody [GT114] (HRP) used in Western Blot (WB). GTX628802-01

GTX628802-01 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (HRP) (GTX628802-01) diluted at 1:1000. The HRP-conjugated anti-FALSE was used to detect the primary antibody.

Anti-alpha Tubulin antibody [GT114] (HRP) used in Western Blot (WB). GTX628802-01

GTX628802-01 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (HRP) (GTX628802-01) diluted at 1:1000. The HRP-conjugated anti-FALSE was used to detect the primary antibody.

Anti-alpha Tubulin antibody [GT114] (HRP) used in Western Blot (WB). GTX628802-01

GTX628802-01 WB Image

alpha Tubulin antibody [GT114] (HRP) detects alpha Tubulin protein by Western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
alpha Tubulin antibody [GT114] (HRP) (GTX628802-01) dilution: 1:10000

REFERENCE

Application Reference

Monta??s-Agudo P et al. J Cell Sci 2021; Inhibition of minor intron splicing reduces Na+ and Ca2+ channel expression and function in cardiomyocytes.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169