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alpha Tubulin antibody [GT114]

GTX628802_42030_20150313_WB_18121411_128.jpg
GTX628802_42030_20161004_IFA_18121411_142.jpg
GTX628802_42030_20160901_WB_M_18121411_387.jpg
GTX628802_42030_20150814_IHC-Fr_M_18121411_506.jpg
GTX628802_42030_20150518_IHC_2_18121411_609.jpg
GTX628802_42030_20150402_IFA_18121411_221.jpg
GTX628802_41295_20151209_IHC-P_M_18121411_333.jpg
GTX628802_42030_20150313_WB_Dose_18121411_682.jpg
GTX628802_42030_20150518_IHC_18121411_310.jpg
GTX628802_41204_WB_Z_eye_18121411_735.jpg
GTX628802_41204_IFA_2_18121411_266.jpg
GTX628802_42926_20170727_WB_Sensitivity_19030801_840.jpg
GTX628802_42030_20160901_WB_R_18121411_290.jpg
GTX628802_41204_WB_multi species_18121411_249.jpg

Cat No. GTX628802

Host Mouse
Clonality Monoclonal
Clone Name GT114
Isotype IgG2a
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat, Zebrafish, Drosophila
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , NIH-3T3 , mouse brain , PC-12 , rat brain , drosophila , zebrafish eye , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , *U87-MG

Predict Reactivity

Rabbit, Bovine, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

K-ALPHA-1 antibody, TUBA1B antibody, alpha-tubulin ubiquitous antibody, alpha tubulin antibody, tubulin K-alpha-1 antibody, tubulin alpha-ubiquitous chain antibody, tubulin, alpha, ubiquitous antibody, tubulin alpha-1B chain antibody, tubulin, alpha 1b antibody

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Research Area

DATA IMAGES
GTX628802_42030_20150313_WB_18121411_128.jpg

GTX628802 WB Image

alpha Tubulin antibody detects alpha Tubulin protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX628802) diluted at a dilution of 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX628802_42030_20161004_IFA_18121411_142.jpg

GTX628802 ICC/IF Image

alpha Tubulin antibody [GT114] detects alpha Tubulin protein expression by immunofluorescent analysis.
Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin protein stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:250.
Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX628802_42030_20160901_WB_M_18121411_387.jpg

GTX628802 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX628802_42030_20150814_IHC-Fr_M_18121411_506.jpg

GTX628802 IHC-Fr Image

Immunofluorescence photomicrographs of frozen sections of mouse brain.
Green: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:200. The signal was developed using goat anti-rmouse IgG antibody (Dylight488) (GTX213111-04).
Blue: Nuclear staining with Hoechst 33342.

GTX628802_42030_20150518_IHC_2_18121411_609.jpg

GTX628802 IHC-P Image

alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoplasm in human cervix by immunohistochemical analysis.
Sample: Paraffin-embedded human cervix.
alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX628802_42030_20150402_IFA_18121411_221.jpg

GTX628802 ICC/IF Image

alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin protein stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX628802_41295_20151209_IHC-P_M_18121411_333.jpg

GTX628802 IHC-P Image

alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoplasm in mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Green: beta Catenin antibody [N1N2-2] (GTX101435) diluted at 1:500.
Blue: Hoechst 33342 staining.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX628802_42030_20150313_WB_Dose_18121411_682.jpg

GTX628802 WB Image

alpha Tubulin antibody detects alpha Tubulin protein by western blot analysis. Varied amount of 293T Whole cell extracts was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX628802) at a dilution of 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX628802_42030_20150518_IHC_18121411_310.jpg

GTX628802 IHC-P Image

alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoplasm in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX628802_41204_WB_Z_eye_18121411_735.jpg

GTX628802 WB Image

alpha Tubulin antibody [GT114] detects TUBA1B protein by western blot analysis.
A. 30 µg zebrafish eye lysate/extract
10% SDS-PAGE
alpha Tubulin antibody [GT114] (GTX628802) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX628802_41204_IFA_2_18121411_266.jpg

GTX628802 ICC/IF Image

TUBA1B antibody detects TUBA1B protein at Cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in -20℃ 100% methanol for 5 min.
Green: TUBA1B protein stained by TUBA1B antibody (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX628802_42926_20170727_WB_Sensitivity_19030801_840.jpg

GTX628802 WB Image

Various whole cell extracts (15 | 5 | 2 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX628802_42030_20160901_WB_R_18121411_290.jpg

GTX628802 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX628802_41204_WB_multi species_18121411_249.jpg

GTX628802 WB Image

alpha Tubulin antibody [GT114] detects TUBA1B protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg NIH-3T3 whole cell lysate/extract
C. 30 µg mouse brain lysate/extract
D. 30 µg PC-12 whole cell lysate/extract
E. 30 µg rat brain lysate/extract
F. 30 µg drosophila lysae/extract
10% SDS-PAGE
alpha Tubulin antibody [GT114] (GTX628802) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE
REVIEW

alpha Tubulin antibody [GT114] Cat No. GTX628802

Rating ( Average 4 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 08-Jun-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : SW480 cells
Amount used : 40 μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:20000, 4°C, overnightHr
Date : Anonymous submitted on 24-Jun-2015
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : U87-MG cells
Blocking : 5% BSA in PBST, 25°C, 2Hr
Fixation : 4% PFA
Primary Antibody : 1:200, 4°C, overnightHr
Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169