PRODUCT
Summary
alpha Tubulin antibody [GT114] detects alpha Tubulin, a cytoskeletal protein widely expressed in cells that is commonly used as a loading control for western blots. The predicted molecular weight of alpha Tubulin protein is 50 kDa. This is a well-cited mouse monoclonal antibody validated for multiple applications including western blot, immunohistochemistry, and immunocytochemistry.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:20000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
293T , mouse brain , rat brain , zebrafish eye , PC-12 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12
Predict Reactivity
Rabbit, Bovine, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
From tissue culture supernatant
Conjugation
Unconjugated
RRID
AB_2716636
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
K-ALPHA-1 antibody , TUBA1B antibody , alpha-tubulin ubiquitous antibody , alpha tubulin antibody , tubulin K-alpha-1 antibody , tubulin alpha-ubiquitous chain antibody , tubulin , alpha , ubiquitous antibody , tubulin alpha-1B chain antibody , tubulin , alpha 1b antibody
Background
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Research Area
DATA IMAGES
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GTX628802 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628802 IHC-P Image
alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoplasm in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. Green: beta Catenin antibody [N1N2-2] (GTX101435) diluted at 1:500. Blue: Hoechst 33342 staining.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX628802 WB Image
alpha Tubulin antibody detects alpha Tubulin protein by western blot analysis. Varied amount of 293T Whole cell extracts was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX628802) at a dilution of 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628802 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628802 ICC/IF Image
TUBA1B antibody detects TUBA1B protein at Cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in -20ºC 100% methanol for 5 min. Green: TUBA1B protein stained by TUBA1B antibody (GTX628802) diluted at 1:500. Blue: Hoechst 33342 staining.
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GTX628802 IHC-P Image
alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoplasm in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX628802 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628802 IHC-Fr Image
Immunofluorescence photomicrographs of frozen sections of mouse brain. Green: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:200. The signal was developed using goat anti-rmouse IgG antibody (Dylight488) (GTX213111-04). Blue: Nuclear staining with Hoechst 33342.
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GTX628802 ICC/IF Image
alpha Tubulin antibody [GT114] detects alpha Tubulin protein expression by immunofluorescent analysis. Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin protein stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:250. Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
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GTX628802 WB Image
alpha Tubulin antibody [GT114] detects TUBA1B protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg NIH-3T3 whole cell lysate/extract C. 30 μg mouse brain lysate/extract D. 30 μg PC-12 whole cell lysate/extract E. 30 μg rat brain lysate/extract F. 30 μg drosophila lysae/extract 10% SDS-PAGE alpha Tubulin antibody [GT114] (GTX628802) dilution: 1:5000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628802 WB Image
alpha Tubulin antibody [GT114] detects TUBA1B protein by western blot analysis. A. 30 μg zebrafish eye lysate/extract 10% SDS-PAGE alpha Tubulin antibody [GT114] (GTX628802) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628802 IHC-P Image
alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoplasm in human cervix by immunohistochemical analysis. Sample: Paraffin-embedded human cervix. alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX628802 ICC/IF Image
alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: alpha Tubulin stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX628802 WB Image
293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628802 WB Image
The data was published in the journal Nat Commun in 2016.PMID: 27221592
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GTX628802 WB Image
The data was published in the 2018 in Sci Rep. PMID: 30459446
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GTX628802 WB Image
The data was published in the 2022 in Int J Mol Sci. PMID: 35055077
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GTX628802 ICC/IF Image
The data was published in the 2018 in Sci Rep. PMID: 30459446
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GTX628802 WB Image
The data was published in the 2022 in Int J Mol Sci. PMID: 35055077
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GTX628802 WB Image
The data was published in the journal Life Sci Alliance in 2019.PMID: 31767615
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GTX628802 WB Image
The data was published in the journal PLoS Biol in 2019.PMID: 30883547
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GTX628802 WB Image
The data was published in the journal Biomolecules in 2020.PMID: 32635653
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GTX628802 WB Image
The data was published in the journal Genome Biol in 2018.PMID: 29548335
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GTX628802 WB Image
The data was published in the journal Biomolecules in 2020.PMID: 32635653
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GTX628802 WB Image
The data was published in the journal PLoS One in 2017.PMID: 28467493
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GTX628802 WB Image
The data was published in the journal Physiol Rep in 2017.PMID: 28408640
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GTX628802 WB Image
The data was published in the journal Nat Commun in 2019.PMID: 31278365
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GTX628802 WB Image
The data was published in the journal Cell Biosci in 2019. PMID: 31044068
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GTX628802 WB Image
The data was published in the journal Toxins (Basel) in 2019. PMID: 31159487
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GTX628802 WB Image
The data was published in the journal Nat Commun in 2020. PMID: 32005813
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GTX628802 WB Image
The data was published in the journal PLoS One in 2015. PMID: 25811469
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GTX628802 WB Image
The data was published in the journal PLoS One in 2014. PMID: 25000307
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GTX628802 WB Image
The data was published in the journal J Cell Mol Med in 2020. PMID: 31821701
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GTX628802 WB Image
The data was published in the journal PLoS One in 2015. PMID: 25811469
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GTX628802 WB Image
The data was published in the journal J Cell Mol Med in 2020. PMID: 31821701
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GTX628802 WB Image
The data was published in the journal PLoS Biol in 2019.PMID: 30883547
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GTX628802 WB Image
The data was published in the journal Genome Biol in 2018.PMID: 29548335
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REFERENCE
REVIEW
alpha Tubulin antibody [GT114]
Cat. No. GTX628802
Rating
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( Average 4 based on 2 users reviews)
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Application
Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Immunocytochemistry/ Immunofluorescence(ICC/IF)
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( Average 3 based on 1 users reviews)
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Date :
Anonymous submitted on 08-Jun-2017
Application Tested :
WB
Sample Species :
Hu
Sample :
SW480 cells
Amount used :
40 μg
Blocking :
5% milk in TBST, 25°C, 1Hr
Primary Antibody :
1:20000, 4°C, 16Hr
Date :
Anonymous submitted on 24-Jun-2015
Application Tested :
ICC/IF
Sample Species :
Hu
Sample :
U87-MG cells
Blocking :
5% BSA in PBST, 25°C, 2Hr
Fixation :
4% PFA
Primary Antibody :
1:200, 4°C, 16Hr