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alpha Tubulin antibody [RM113]

Anti-alpha Tubulin antibody [RM113] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33609
Anti-alpha Tubulin antibody [RM113] used in Western Blot (WB). GTX33609

Cat No. GTX33609

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 RM113

Isotype

 IgG

Application

 WB, ICC/IF, FACS, IP, ELISA, IHC

Reactivity

 Human
Package
100 μl ($329)
See all alpha Tubulin products

APPLICATION

Application Note

Western Blot (WB): 1:1000 dilution, Immunoprecipitation (IP): 1:200 dilution, Chromatin IP (ChIP): 1:200 dilution, Immunocytochemistry (ICC): 1:200 dilution, Immunohistochemistry (IHC): 1:200 dilution.

Specificity/Sensitivity

This antibody reacts to α-Tubulin, including Tubulin alpha-1A chain and Tubulin alpha-1B chain.

PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Immunogen

A peptide corresponding to the C-terminus of α-Tubulin

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

Anti-alpha Tubulin antibody [RM113] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33609

GTX33609 ICC/IF Image

Immunocytochemical staining of HeLa cells, using Anti-α-Tubulin Clone RM113 at 1/200 dilution (red). Nuclei have been labeled with DAPI (blue).

Anti-alpha Tubulin antibody [RM113] used in Western Blot (WB). GTX33609

GTX33609 WB Image

Western blot of A431 cells, using Anti-α-Tubulin Clone RM113 at 1/1000 dilution, showed a band of α-Tubulin at the predicted MW (~50 kDa).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329