GeneTex
United States (US)

alpha Tubulin antibody [TU-01]

alpha-Tubulin-antibody-TU-01__GTX27750-1_18121409_413.jpg
GTX27750_WB_18121410_935.jpg
alpha-Tubulin-antibody-TU-01__GTX27750-2_18121409_256.jpg
alpha-Tubulin-antibody-TU-01__GTX27750-3_18121409_532.jpg
GTX27750_ICCIF_18121410_542.jpg

Cat No. GTX27750

Host Mouse
Clonality Monoclonal
Clone Name TU-01
Isotype IgG1
Application WB, ICC/IF, IHC-P, IP, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Pig

Immunogen

Fraction of tubulin purified from pig brain by two cycles of polymerization-depolymerization.

Purification

Purified by precipitation methods

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

TUBA1 Tubulin alpha 1 chain Tubulin alpha

Cellular Localization

Major constituent of microtubules.

Background

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. Tubulin is a heterodimer which consists of alpha tubulin and beta tubulin.
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Research Area

DATA IMAGES
alpha-Tubulin-antibody-TU-01__GTX27750-1_18121409_413.jpg

GTX27750 ICC/IF Image

Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-tubulin (GTX27750; green) and anti-Vimentin (GTX27752; red). Nucleus is stained with DAPI (blue)

GTX27750_WB_18121410_935.jpg

GTX27750 IP Image

IP of alpha Tubulin from HeLa and Raji lysates by alpha Tubulin antibody [TU-16] was performed. The precipitated protein was detected by alpha Tubulin antibody [TU-01].

alpha-Tubulin-antibody-TU-01__GTX27750-2_18121409_256.jpg

GTX27750 ICC/IF Image

Immunofluorescence staining of HeLa human cervix carcinoma cell line using anti-alpha-tubulin (GTX27750; red). Nucleus is stained with DAPI (blue).

alpha-Tubulin-antibody-TU-01__GTX27750-3_18121409_532.jpg

GTX27750 WB Image

Use of anti-alpha-tubulin antibody GTX27750 as a loading control (A) in an Western blot experiment revealing the staining pattern of various cell lysates by a newly developed monoclonal antibody (B)

GTX27750_ICCIF_18121410_542.jpg

GTX27750 ICC/IF Image

ICC/IF analysis of 3T3 cells using alpha Tubulin antibody [TU-01] (green) and DAPI (blue).