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alpha Tubulin antibody [YOL1/34]

Anti-alpha Tubulin antibody [YOL1/34] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26161
Anti-alpha Tubulin antibody [YOL1/34] used in Western Blot (WB). GTX26161

Cat. No. GTX26161

Reference 2

Host

 Rat

Clonality

 Monoclonal

Clone Name

 YOL1/34

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-Fr, ELISA, RIA

Reactivity

 Human, Mouse, Yeast, Drosophila, Arabidopsis thaliana, Ashbya, Asplenium nidus, Naegleria, Platyzoma, Seagrass (Cymodocea nodosa)
Package
100 μg ($399)
See all alpha Tubulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
ELISA 10μg/ml (as detecting antibody)
RIA Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Yeast

Immunogen

Yeast tubulin

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_385177

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-alpha Tubulin antibody [YOL1/34] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26161

GTX26161 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX26161 alpha Tubulin antibody [YOL1/34].

Anti-alpha Tubulin antibody [YOL1/34] used in Western Blot (WB). GTX26161

GTX26161 WB Image

Western blot analysis of CD107b on J774 cell lysate probed with Rat anti Mouse CD107b (GTX42498) at 1/500, 1/1000, 1/2000. Rat anti tubulin alpha (GTX26161) is included as a loading control.

REFERENCE

Application Reference

Doi G et al. Nucleic Acids Res 2021; 49 (2) : 954-968 Catalytically inactive Cas9 impairs DNA replication fork progression to induce focal genomic instability.
Application : WB
Reactivity : Yeast
Zhang H et al. Methods Mol Biol 2004; 241 : 77-91 Analysis of the budding yeast Saccharomyces cerevisiae cell cycle by morphological criteria and flow cytometry.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399