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alpha Tubulin antibody [YOL1/34]

Anti-alpha Tubulin antibody [YOL1/34] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26161
Anti-alpha Tubulin antibody [YOL1/34] used in Western Blot (WB). GTX26161

Cat. No. GTX26161

Reference 2

Host

 Rat

Clonality

 Monoclonal

Clone Name

 YOL1/34

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-Fr, ELISA, RIA

Reactivity

 Human, Mouse, Yeast, Drosophila, Arabidopsis thaliana, Ashbya, Asplenium nidus, Naegleria, Platyzoma, Seagrass (Cymodocea nodosa)
Package
100 μg ($399)
See all alpha Tubulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
ELISA 10μg/ml (as detecting antibody)
RIA Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Yeast

Immunogen

Yeast tubulin

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_385177

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

Anti-alpha Tubulin antibody [YOL1/34] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26161

GTX26161 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX26161 alpha Tubulin antibody [YOL1/34].

Anti-alpha Tubulin antibody [YOL1/34] used in Western Blot (WB). GTX26161

GTX26161 WB Image

Western blot analysis of CD107b on J774 cell lysate probed with Rat anti Mouse CD107b (GTX42498) at 1/500, 1/1000, 1/2000. Rat anti tubulin alpha (GTX26161) is included as a loading control.

REFERENCE

Application Reference

Doi G et al. Nucleic Acids Res 2021; 49 (2) : 954-968 Catalytically inactive Cas9 impairs DNA replication fork progression to induce focal genomic instability.
Application : WB
Reactivity : Yeast
Zhang H et al. Methods Mol Biol 2004; 241 : 77-91 Analysis of the budding yeast Saccharomyces cerevisiae cell cycle by morphological criteria and flow cytometry.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399