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alpha Tubulin antibody

Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102078
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102078
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102078
Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX102078

Cat No. GTX102078

Reference 11

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all alpha Tubulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Zebrafish, Rabbit, Bovine, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.14 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

K-ALPHA-1 antibody , TUBA1B antibody , alpha-tubulin ubiquitous antibody , alpha tubulin antibody , tubulin K-alpha-1 antibody , tubulin alpha-ubiquitous chain antibody , "tubulin , alpha , ubiquitous antibody" , tubulin alpha-1B chain antibody , "tubulin , alpha 1b antibody"

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Research Area

DATA IMAGES

Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102078

GTX102078 ICC/IF Image

alpha Tubulin antibody detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin protein stained by alpha Tubulin antibody (GTX102078) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102078

GTX102078 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX102078) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102078

GTX102078 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX102078) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX102078

GTX102078 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U251 xenograft, using alpha Tubulin(GTX102078) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Cheng YP et al. Int J Mol Sci 2019; 20 (13) Blockade of STAT3 Signaling Contributes to Anticancer Effect of 5-Acetyloxy-6,7,8,4'-Tetra-Methoxyflavone, a Tangeretin Derivative, on Human Glioblastoma Multiforme Cells.
Application : WB
Reactivity : Human
Velasco MX et al. RNA 2019; (Epub) Antagonism between the RNA binding protein Musashi1 and miR-137 and its potential impact on neurogenesis and glioblastoma development.
Application : WB
Reactivity : Human
Su CF et al. Brain Res 2018; 1678 : 397-406 Application of amniotic fluid stem cells in repairing sciatic nerve injury in minipigs.
Application : WB
Reactivity : Human
Chang WT et al. Molecules 2017; 22 (5) A 4-Phenoxyphenol Derivative Exerts Inhibitory Effects on Human Hepatocellular Carcinoma Cells through Regulating Autophagy and Apoptosis Accompanied by Downregulating α-Tubulin Expression.
Shimada M et al. 2017; 47 : 21-27 Dietary supplementation with myo-inositol reduces hepatic triglyceride accumulation and expression of both fructolytic and lipogenic genes in rats fed a high-fructose diet.
Application : WB
Reactivity : Rat
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REVIEW

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Application Reference
Cheng YP et al. Int J Mol Sci 2019; 20 (13) Blockade of STAT3 Signaling Contributes to Anticancer Effect of 5-Acetyloxy-6,7,8,4'-Tetra-Methoxyflavone, a Tangeretin Derivative, on Human Glioblastoma Multiforme Cells.
Application : WB
Reactivity : Human
Velasco MX et al. RNA 2019; (Epub) Antagonism between the RNA binding protein Musashi1 and miR-137 and its potential impact on neurogenesis and glioblastoma development.
Application : WB
Reactivity : Human
Su CF et al. Brain Res 2018; 1678:397-406 Application of amniotic fluid stem cells in repairing sciatic nerve injury in minipigs.
Application : WB
Reactivity : Human
Chang WT et al. Molecules 2017; 22 (5) A 4-Phenoxyphenol Derivative Exerts Inhibitory Effects on Human Hepatocellular Carcinoma Cells through Regulating Autophagy and Apoptosis Accompanied by Downregulating α-Tubulin Expression.
Shimada M et al. 2017; 47:21-27 Dietary supplementation with myo-inositol reduces hepatic triglyceride accumulation and expression of both fructolytic and lipogenic genes in rats fed a high-fructose diet.
Application : WB
Reactivity : Rat
Lee YH et al. Oncotarget 2016; 7 (40):65589-65601 Paradoxical overexpression of MBNL2 in hepatocellular carcinoma inhibits tumor growth and invasion.
Application : WB
Reactivity : Human
Chang WT et al. Anticancer Agents Med Chem 2016; (Epub) 9-bis[2-(pyrrolidin-1-yl)ethoxy]-6-{4-[2-(pyrrolidin-1-yl)ethoxy]phenyl}-11H-indeno[1,2-c]quinolin-11-one (BPIQ), a quinoline derivative inhibits human hepatocellular carcinoma cells by inducing ER stress and apoptosis.
Application : WB
Reactivity : Human
Chen S et al. Mol Neurobiol 2016; (Epub) MARK2/Par1b Insufficiency Attenuates DVL Gene Transcription via Histone Deacetylation in Lumbosacral Spina Bifida.
Application : WB
Reactivity : Mouse
Chen YA et al. Sci Rep 2015; 5 (Epub):10667 The Cullin 4A/B-DDB1-Cereblon E3 Ubiquitin Ligase Complex Mediates the Degradation of CLC-1 Chloride Channels.
Application : WB
Reactivity : Human
Ko CC et al. J Biol Chem 2014; Chemical proteomics identifies heterogeneous nuclear ribonucleoprotein (hnRNP) A1 as the molecular target of Quercetin in its anti-cancer effects in PC-3 cells.
Application : WB
Reactivity : Human
Chen J et al. Stem Cell Res Ther 2014; 5 (2):36 Thrombin promotes fibronectin secretion by bone marrow mesenchymal stem cells via the protease-activated receptor mediated signalling pathways.
Application : WB, ICC/IF
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169