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alpha Tubulin antibody

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102078
Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX102078
Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102078
Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102078
Anti-alpha Tubulin antibody used in IHC (Whole mount) (IHC-Wm). GTX102078
Anti-alpha Tubulin antibody used in IHC (Whole mount) (IHC-Wm). GTX102078
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102078
Anti-alpha Tubulin antibody used in IHC (Whole mount) (IHC-Wm). GTX102078
Anti-alpha Tubulin antibody used in IHC (Whole mount) (IHC-Wm). GTX102078
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102078
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102078
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102078
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102078
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102078
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102078

Cat. No. GTX102078

Reference 13

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all alpha Tubulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rabbit, Bovine, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.14 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10731277

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

K-ALPHA-1 antibody , TUBA1B antibody , alpha-tubulin ubiquitous antibody , alpha tubulin antibody , tubulin K-alpha-1 antibody , tubulin alpha-ubiquitous chain antibody , "tubulin , alpha , ubiquitous antibody" , tubulin alpha-1B chain antibody , "tubulin , alpha 1b antibody"

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Research Area

DATA IMAGES

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102078

GTX102078 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX102078) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX102078

GTX102078 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U251 xenograft, using alpha Tubulin(GTX102078) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102078

GTX102078 ICC/IF Image

alpha Tubulin antibody detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin protein stained by alpha Tubulin antibody (GTX102078) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102078

GTX102078 ICC/IF Image

alpha Tubulin antibody detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin stained by alpha Tubulin antibody (GTX102078) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-alpha Tubulin antibody used in IHC (Whole mount) (IHC-Wm). GTX102078

GTX102078 IHC-Wm Image

alpha Tubulin antibody detects alpha Tubulin protein on zebrafish by whole mount immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.
alpha Tubulin antibody (GTX102078) dilution: 1:200.

Anti-alpha Tubulin antibody used in IHC (Whole mount) (IHC-Wm). GTX102078

GTX102078 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using alpha Tubulin antibody (GTX102078) at 1:200 dilution.

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102078

GTX102078 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX102078) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in IHC (Whole mount) (IHC-Wm). GTX102078

GTX102078 IHC-Wm Image

alpha Tubulin antibody detects alpha Tubulin protein at axons on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
alpha Tubulin antibody (GTX102078) dilution: 1:200.
Anti-alpha Tubulin antibody used in IHC (Whole mount) (IHC-Wm). GTX102078

GTX102078 IHC-Wm Image

alpha Tubulin antibody detects alpha Tubulin protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
alpha Tubulin antibody (GTX102078) dilution: 1:200.
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102078

GTX102078 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX102078) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102078

GTX102078 WB Image

The data was published in the journal Int J Mol Sci in 2019. PMID: 31323961

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102078

GTX102078 WB Image

The data was published in the journal Int J Mol Sci in 2019. PMID: 31323961

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102078

GTX102078 WB Image

The data was published in the journal Oncotarget in 2016. PMID: 27564110

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102078

GTX102078 WB Image

The data was published in the journal Oncotarget in 2016. PMID: 27564110

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102078

GTX102078 WB Image

The data was published in the journal Molecules in 2017. PMID: 28531143

REFERENCE

Application Reference

Nyabuto GO et al. Cancers (Basel) 2021; 13 (22) The Large GTPase, GBP-2, Regulates Rho Family GTPases to Inhibit Migration and Invadosome Formation in Breast Cancer Cells.
Application : WB
Reactivity : Human
Mahdee AF et al. Europe PMC 2021; (Epub) Structural and Functional Relations between the Connective Tissue and Epithelium of Enamel Organ and their Role during Enamel Maturation.
Cheng YP et al. Int J Mol Sci 2019; 20 (13) Blockade of STAT3 Signaling Contributes to Anticancer Effect of 5-Acetyloxy-6,7,8,4'-Tetra-Methoxyflavone, a Tangeretin Derivative, on Human Glioblastoma Multiforme Cells.
Application : WB
Reactivity : Human
Velasco MX et al. RNA 2019; (Epub) Antagonism between the RNA binding protein Musashi1 and miR-137 and its potential impact on neurogenesis and glioblastoma development.
Application : WB
Reactivity : Human
Su CF et al. Brain Res 2018; 1678 : 397-406 Application of amniotic fluid stem cells in repairing sciatic nerve injury in minipigs.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Nyabuto GO et al. Cancers (Basel) 2021; 13 (22) The Large GTPase, GBP-2, Regulates Rho Family GTPases to Inhibit Migration and Invadosome Formation in Breast Cancer Cells.
Application : WB
Reactivity : Human
Mahdee AF et al. Europe PMC 2021; (Epub) Structural and Functional Relations between the Connective Tissue and Epithelium of Enamel Organ and their Role during Enamel Maturation.
Cheng YP et al. Int J Mol Sci 2019; 20 (13) Blockade of STAT3 Signaling Contributes to Anticancer Effect of 5-Acetyloxy-6,7,8,4'-Tetra-Methoxyflavone, a Tangeretin Derivative, on Human Glioblastoma Multiforme Cells.
Application : WB
Reactivity : Human
Velasco MX et al. RNA 2019; (Epub) Antagonism between the RNA binding protein Musashi1 and miR-137 and its potential impact on neurogenesis and glioblastoma development.
Application : WB
Reactivity : Human
Su CF et al. Brain Res 2018; 1678:397-406 Application of amniotic fluid stem cells in repairing sciatic nerve injury in minipigs.
Application : WB
Reactivity : Human
Chang WT et al. Molecules 2017; 22 (5) A 4-Phenoxyphenol Derivative Exerts Inhibitory Effects on Human Hepatocellular Carcinoma Cells through Regulating Autophagy and Apoptosis Accompanied by Downregulating α-Tubulin Expression.
Shimada M et al. 2017; 47:21-27 Dietary supplementation with myo-inositol reduces hepatic triglyceride accumulation and expression of both fructolytic and lipogenic genes in rats fed a high-fructose diet.
Application : WB
Reactivity : Rat
Lee YH et al. Oncotarget 2016; 7 (40):65589-65601 Paradoxical overexpression of MBNL2 in hepatocellular carcinoma inhibits tumor growth and invasion.
Application : WB
Reactivity : Human
Chang WT et al. Anticancer Agents Med Chem 2016; (Epub) 9-bis[2-(pyrrolidin-1-yl)ethoxy]-6-{4-[2-(pyrrolidin-1-yl)ethoxy]phenyl}-11H-indeno[1,2-c]quinolin-11-one (BPIQ), a quinoline derivative inhibits human hepatocellular carcinoma cells by inducing ER stress and apoptosis.
Application : WB
Reactivity : Human
Chen S et al. Mol Neurobiol 2016; (Epub) MARK2/Par1b Insufficiency Attenuates DVL Gene Transcription via Histone Deacetylation in Lumbosacral Spina Bifida.
Application : WB
Reactivity : Mouse
Chen YA et al. Sci Rep 2015; 5 (Epub):10667 The Cullin 4A/B-DDB1-Cereblon E3 Ubiquitin Ligase Complex Mediates the Degradation of CLC-1 Chloride Channels.
Application : WB
Reactivity : Human
Ko CC et al. J Biol Chem 2014; Chemical proteomics identifies heterogeneous nuclear ribonucleoprotein (hnRNP) A1 as the molecular target of Quercetin in its anti-cancer effects in PC-3 cells.
Application : WB
Reactivity : Human
Chen J et al. Stem Cell Res Ther 2014; 5 (2):36 Thrombin promotes fibronectin secretion by bone marrow mesenchymal stem cells via the protease-activated receptor mediated signalling pathways.
Application : WB, ICC/IF
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169