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alpha Tubulin antibody

Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102078
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102078
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102078
Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX102078

Cat No. GTX102078

Reference 10
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
See all alpha Tubulin products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Zebrafish, Rabbit, Bovine, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

K-ALPHA-1 antibody, TUBA1B antibody, alpha-tubulin ubiquitous antibody, alpha tubulin antibody, tubulin K-alpha-1 antibody, tubulin alpha-ubiquitous chain antibody, "tubulin, alpha, ubiquitous antibody", tubulin alpha-1B chain antibody, "tubulin, alpha 1b antibody"

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Research Area

DATA IMAGES
Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102078

GTX102078 ICC/IF Image

alpha Tubulin antibody detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin protein stained by alpha Tubulin antibody (GTX102078) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102078

GTX102078 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX102078) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102078

GTX102078 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX102078) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX102078

GTX102078 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U251 xenograft, using alpha Tubulin(GTX102078) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
REFERENCE

Application Reference

Cheng YP et al. Int J Mol Sci 2019; 20 (13) Blockade of STAT3 Signaling Contributes to Anticancer Effect of 5-Acetyloxy-6,7,8,4'-Tetra-Methoxyflavone, a Tangeretin Derivative, on Human Glioblastoma Multiforme Cells.
Application : WB
Reactivity : Human
Velasco MX et al. RNA 2019; (Epub) Antagonism between the RNA binding protein Musashi1 and miR-137 and its potential impact on neurogenesis and glioblastoma development.
Application : WB
Reactivity : Human
Su CF et al. Brain Res 2018; 1678 : 397-406 Application of amniotic fluid stem cells in repairing sciatic nerve injury in minipigs.
Application : WB
Reactivity : Human
Shimada M et al. 2017; 47 : 21-27 Dietary supplementation with myo-inositol reduces hepatic triglyceride accumulation and expression of both fructolytic and lipogenic genes in rats fed a high-fructose diet.
Application : WB
Reactivity : Rat
Lee YH et al. Oncotarget 2016; 7 (40) : 65589-65601 Paradoxical overexpression of MBNL2 in hepatocellular carcinoma inhibits tumor growth and invasion.
Application : WB
Reactivity : Human
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REVIEW
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Application Reference
Cheng YP et al. Int J Mol Sci 2019; 20 (13) Blockade of STAT3 Signaling Contributes to Anticancer Effect of 5-Acetyloxy-6,7,8,4'-Tetra-Methoxyflavone, a Tangeretin Derivative, on Human Glioblastoma Multiforme Cells.
Application : WB
Reactivity : Human
Velasco MX et al. RNA 2019; (Epub) Antagonism between the RNA binding protein Musashi1 and miR-137 and its potential impact on neurogenesis and glioblastoma development.
Application : WB
Reactivity : Human
Su CF et al. Brain Res 2018; 1678:397-406 Application of amniotic fluid stem cells in repairing sciatic nerve injury in minipigs.
Application : WB
Reactivity : Human
Shimada M et al. 2017; 47:21-27 Dietary supplementation with myo-inositol reduces hepatic triglyceride accumulation and expression of both fructolytic and lipogenic genes in rats fed a high-fructose diet.
Application : WB
Reactivity : Rat
Lee YH et al. Oncotarget 2016; 7 (40):65589-65601 Paradoxical overexpression of MBNL2 in hepatocellular carcinoma inhibits tumor growth and invasion.
Application : WB
Reactivity : Human
Chang WT et al. Anticancer Agents Med Chem 2016; (Epub) 9-bis[2-(pyrrolidin-1-yl)ethoxy]-6-{4-[2-(pyrrolidin-1-yl)ethoxy]phenyl}-11H-indeno[1,2-c]quinolin-11-one (BPIQ), a quinoline derivative inhibits human hepatocellular carcinoma cells by inducing ER stress and apoptosis.
Application : WB
Reactivity : Human
Chen S et al. Mol Neurobiol 2016; (Epub) MARK2/Par1b Insufficiency Attenuates DVL Gene Transcription via Histone Deacetylation in Lumbosacral Spina Bifida.
Application : WB
Reactivity : Mouse
Chen YA et al. Sci Rep 2015; 5 (Epub):10667 The Cullin 4A/B-DDB1-Cereblon E3 Ubiquitin Ligase Complex Mediates the Degradation of CLC-1 Chloride Channels.
Application : WB
Reactivity : Human
Ko CC et al. J Biol Chem 2014; Chemical proteomics identifies heterogeneous nuclear ribonucleoprotein (hnRNP) A1 as the molecular target of Quercetin in its anti-cancer effects in PC-3 cells.
Application : WB
Reactivity : Human
Chen J et al. Stem Cell Res Ther 2014; 5 (2):36 Thrombin promotes fibronectin secretion by bone marrow mesenchymal stem cells via the protease-activated receptor mediated signalling pathways.
Application : WB, ICC/IF
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169