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alpha Tubulin antibody

Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX102079
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102079
Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102079
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102079

Cat No. GTX102079

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Zebrafish, Rabbit, Bovine, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

K-ALPHA-1 antibody , TUBA1B antibody , alpha-tubulin ubiquitous antibody , alpha tubulin antibody , tubulin K-alpha-1 antibody , tubulin alpha-ubiquitous chain antibody , "tubulin , alpha , ubiquitous antibody" , tubulin alpha-1B chain antibody , "tubulin , alpha 1b antibody"

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Research Area

DATA IMAGES

Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX102079

GTX102079 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using alpha Tubulin(GTX102079) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102079

GTX102079 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
10% SDS PAGE
GTX102079 diluted at 1:10000

Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102079

GTX102079 ICC/IF Image

alpha Tubulin antibody detects TUBA1B protein at cytoskeleton by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TUBA1B protein stained by alpha Tubulin antibody (GTX102079) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102079

GTX102079 WB Image

The WB analysis of alpha Tubulin antibody was published by Thiebaut C and colleagues in the journal PLoS One in 2017.PMID: 28301550

REFERENCE

REVIEW

alpha Tubulin antibody Cat No. GTX102079

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 28-Dec-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : primary culture endothelial cells
Amount used : 5μg
Blocking : 3% BSA in TBST, 23°C, 2Hr
Primary Antibody : 1:2000, 25°C, 2Hr
SDS
Glycerol.pdf
Thimerosal.pdf