alpha Tubulin antibody

Name: alpha Tubulin antibody
Brand: GeneTex
SKU: GTX102079
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX102079

Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102079

Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX102079

Anti-alpha Tubulin antibody used in IHC (Whole mount) (IHC-Wm). GTX102079

Cat. No. GTX102079

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Wm
Reactivity	Human, Zebrafish, Drosophila
Package	100 μl ($399), 25 μl ($169)

See all alpha Tubulin products

View product citations for antibody GTX102079 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5000-1:20000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Wm	Assay dependent

Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163195

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

K-ALPHA-1 antibody , TUBA1B antibody , alpha-tubulin ubiquitous antibody , alpha tubulin antibody , tubulin K-alpha-1 antibody , tubulin alpha-ubiquitous chain antibody , "tubulin , alpha , ubiquitous antibody" , tubulin alpha-1B chain antibody , "tubulin , alpha 1b antibody"

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Research Area

DATA IMAGES

GTX102079 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX102079) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Drosophila alphaTub85E is the homolog of mammalian alpha Tubulin. (Gene ID: 41183/ UniProt: P06604)

GTX102079 ICC/IF Image

alpha Tubulin antibody detects TUBA1B protein at cytoskeleton by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TUBA1B protein stained by alpha Tubulin antibody (GTX102079) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX102079 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
10% SDS PAGE
GTX102079 diluted at 1:10000

GTX102079 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using alpha Tubulin(GTX102079) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102079 IHC-Wm Image

alpha Tubulin antibody detects alpha Tubulin protein on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
alpha Tubulin antibody (GTX102079) dilution: 1:200.

GTX102079 WB Image

The data was published in the journal PLoS One in 2017. PMID: 28301550

REFERENCE

Application Reference

Marine Geoffroy et al. Int J Mol Sci 2022; 23 (12) AB186 Inhibits Migration of Triple-Negative Breast Cancer Cells and Interacts with α-Tubulin.

Application : ICC/IF

Reactivity : Human

Geoffroy M et al. Mol Biol Rep 2020; 47 (10) : 7643-7653 Claudin 1 inhibits cell migration and increases intercellular adhesion in triple-negative breast cancer cell line.

Application : WB

Reactivity : Human

Thiebaut C et al. Int J Mol Sci 2019; 20 (11) Dual Epigenetic Regulation of ER帢36 Expression in Breast Cancer Cells.

Thiebaut C et al. PLoS One 2017; 12 (3) : e0173931 Mammary epithelial cell phenotype disruption in vitro and in vivo through ERalpha36 overexpression.

Application : WB

Reactivity : Human

Berthe A et al. Cell Cycle 2016; 15 (24) : 3402-3412 Δ2-Troglitazone promotes cytostatic rather than pro-apoptotic effects in breast cancer cells cultured in high serum conditions.

Application : WB

Reactivity : Human

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