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alpha Tubulin antibody

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112141
Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112141
Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112141
Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112141
Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

Cat. No. GTX112141

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat, Drosophila, Chicken, Caenorhabditis elegans, Mosquito, Tetrahymena
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Positive Control

PC-12 , Rat-2 , 293T , A431 , HeLa , HepG2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , Jurkat , Raji

Predict Reactivity

Sheep, Bovine, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722892

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

DATA IMAGES

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX112141) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX112141) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX112141) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX112141) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112141

GTX112141 IHC-P Image

alpha Tubulin antibody detects alpha Tubulin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat esophagus.
alpha Tubulin stained by alpha Tubulin antibody (GTX112141) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112141

GTX112141 IHC-P Image

alpha Tubulin antibody detects alpha Tubulin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
alpha Tubulin stained by alpha Tubulin antibody (GTX112141) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

alpha Tubulin antibody detects alpha Tubulin protein by western blot analysis.
A. 30 μg Drosophila lysate/extract
10% SDS-PAGE
alpha Tubulin antibody (GTX112141) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112141

GTX112141 ICC/IF Image

alpha Tubulin antibody detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin stained by alpha Tubulin antibody (GTX112141) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX112141) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112141

GTX112141 ICC/IF Image

alpha Tubulin antibody detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin stained by alpha Tubulin antibody (GTX112141) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

Sample (30 μg of cell lysate)
A: HeLa
B: HeLa cytosol fraction
C: HeLa nucleus fraction
10% SDS PAGE
GTX112141 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112141

GTX112141 IHC-P Image

alpha Tubulin antibody detects alpha Tubulin protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
alpha Tubulin stained by alpha Tubulin antibody (GTX112141) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112141

GTX112141 IHC-P Image

alpha Tubulin antibody detects alpha Tubulin protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
alpha Tubulin stained by alpha Tubulin antibody (GTX112141) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal Sci Rep in 2019. PMID: 31723205

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the 2019 in Cancers (Basel). PMID: 31861872

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal PLoS One in 2014. PMID: 25360769

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal Front Oncol in 2019. PMID: 31024851

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal PLoS One in 2016. PMID: 27537547

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal PLoS One in 2016. PMID: 27537547

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28630472

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal Sci Rep in 2016. PMID: 26838546

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal Proteome Sci in 2014. PMID: 24405781

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal PLoS One in 2015. PMID: 26161782

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28630472

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the 2019 in J Clin Med. PMID: 31121901

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal Int J Mol Sci in 2019. PMID: 30678307

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal Cell Death Dis in 2015. PMID: 25950483

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal Oncogene in 2017. PMID: 28436950

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal PLoS One in 2014. PMID: 25360769

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal Front Oncol in 2019. PMID: 31024851

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal Nat Commun in 2019. PMID: 30622262

Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112141

GTX112141 ICC/IF Image

The data was published in the journal PLoS One in 2014. PMID: 25360769

Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112141

GTX112141 ICC/IF Image

The data was published in the journal Cell Div in 2019. PMID: 31110557

Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112141

GTX112141 ICC/IF Image

The data was published in the journal Calcif Tissue Int in 2017. PMID: 28097375

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal PLoS One in 2015.PMID: 25993510

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal Cell Discov in 2015.PMID: 27462424

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal PLoS One in 2015.PMID: 25993510

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

The data was published in the journal Front Mol Neurosci in 2017.PMID: 28676741

REFERENCE

REVIEW

alpha Tubulin antibody Cat. No. GTX112141

Rating ( Average 4.5 based on 6 users reviews)
Application
Western Blot(WB) ( Average 4.8 based on 5 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 14-Feb-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa and MCF-7 cell lysates
Amount used : 20μg
Blocking : 5% Skim Milk in PBS, 25°C, 1Hr
Primary Antibody : 1:1000, 1°C, 25Hr
Date : Anonymous submitted on 09-Sep-2021
Score :
Application Tested : ICC/IF
Sample Species : Tetrahymena
Sample : Tetrahymena
Blocking : 10% Donkey serum in PBS, 20°C, 0.5Hr
Fixation : 2% PFA in PHEM buffer (PEHEM; 60 mM PIPES, 25 mM HEPES, 10 mM EGTA, 4 mM MgSO4, pH 6.9)
Permeabilization /Antigen retrieval : 0.5% Triton-X in PBS
Primary Antibody : 1:800, 20°C, 1Hr
Figure Note : A projection image of the whole cell was constructed from 96 optical sections obtained at 0.2 μm intervals.
Date : Anonymous submitted on 07-Aug-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : Caco2 cells
Amount used : 25 ㎍
Blocking : 3% BSA, 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 1Hr
Figure Note : Image courtesy of Nao Sugihara from National Defense Medical College.
Date : Anonymous submitted on 13-Feb-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells, HCT116 cells
Amount used : 50μg
Blocking : 1% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 14Hr
Lane Description : Lane1:HeLa cells Lane2:HCT116 cells
Date : Anonymous submitted on 16-Jan-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells,Mahlavu cells,Huh6 cells
Amount used : 20μg
Blocking : 5% non-fat milk in TBST, 4°C, overnightHr
Primary Antibody : 1:5000, 25°C, 1Hr
Date : Anonymous submitted on 08-Dec-2011
Score :
Application Tested : WB
Sample Species : Ms
Sample : Y1 cells
Blocking : 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 18Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169