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alpha Tubulin antibody

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141
Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112141
Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112141
Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112141
Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

Cat No. GTX112141

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat, Drosophila, Chicken, Mosquito, Tetrahymena
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Positive Control

293T , A431 , HeLa , HepG2 , Jurkat , Raji , HeLa cytosol fraction , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2 , Drosophila

Predict Reactivity

Sheep, Bovine, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS,20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722892

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX112141) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

alpha Tubulin antibody detects alpha Tubulin protein by western blot analysis.
A. 30 μg Drosophila lysate/extract
10% SDS-PAGE
alpha Tubulin antibody (GTX112141) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

Sample (30 μg of cell lysate)
A: HeLa
B: HeLa cytosol fraction
C: HeLa nucleus fraction
10% SDS PAGE
GTX112141 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX112141) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX112141) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX112141) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112141

GTX112141 IHC-P Image

alpha Tubulin antibody detects alpha Tubulin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat esophagus.
alpha Tubulin stained by alpha Tubulin antibody (GTX112141) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Tubulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112141

GTX112141 IHC-P Image

alpha Tubulin antibody detects alpha Tubulin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
alpha Tubulin stained by alpha Tubulin antibody (GTX112141) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Tubulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112141

GTX112141 ICC/IF Image

alpha Tubulin antibody detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin stained by alpha Tubulin antibody (GTX112141) diluted at 1:500.

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX112141

GTX112141 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX112141) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

alpha Tubulin antibody Cat No. GTX112141

Rating ( Average 4.9 based on 28 users reviews)
Application
Western Blot(WB) ( Average 5 based on 24 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4.5 based on 4 users reviews)
Date : Anonymous submitted on 09-Sep-2021
Score :
Application Tested : ICC/IF
Sample Species : Tetrahymena
Sample : Tetrahymena
Blocking : 10% Donkey serum in PBS, 20°C, 0.5Hr
Fixation : 2% PFA in PHEM buffer (PEHEM; 60 mM PIPES, 25 mM HEPES, 10 mM EGTA, 4 mM MgSO4, pH 6.9)
Permeabilization /Antigen retrieval : 0.5% Triton-X in PBS
Primary Antibody : 1:800, 20°C, 1Hr
Figure Note : A projection image of the whole cell was constructed from 96 optical sections obtained at 0.2 μm intervals.
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015.PMID: 25993510
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cell Discov in 2015.PMID: 27462424
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Front Mol Neurosci in 2017.PMID: 28676741
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015.PMID: 25993510
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2016.PMID: 27537547
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2016.PMID: 27537547
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015.PMID: 26161782
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28630472
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016.PMID: 26838546
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28630472
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Proteome Sci in 2014.PMID: 24405781
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Front Oncol in 2019.PMID: 31024851
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2019.PMID: 30678307
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncogene in 2017.PMID: 28436950
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2015.PMID: 25950483
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014.PMID: 25360769
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014.PMID: 25360769
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Front Oncol in 2019.PMID: 31024851
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2019.PMID: 30622262
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cell Div in 2019.PMID: 31110557
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Calcif Tissue Int in 2017.PMID: 28097375
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014.PMID: 25360769
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2019.PMID: 31723205
Date : Anonymous submitted on 07-Aug-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : Caco2 cells
Amount used : 25 ㎍
Blocking : 3% BSA, 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 1Hr
Figure Note : Image courtesy of Nao Sugihara from National Defense Medical College.
Date : Anonymous submitted on 13-Feb-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells, HCT116 cells
Amount used : 50μg
Blocking : 1% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 14Hr
Lane Description : Lane1:HeLa cells Lane2:HCT116 cells
Date : Anonymous submitted on 16-Jan-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : Huh7 cells,Mahlavu cells,Huh6 cells
Amount used : 20μg
Blocking : 5% non-fat milk in TBST, 4°C, overnightHr
Primary Antibody : 1:5000, 25°C, 1Hr
Date : Anonymous submitted on 08-Dec-2011
Score :
Application Tested : WB
Sample Species : Ms
Sample : Y1 cells
Blocking : 25°C, 1Hr
Primary Antibody : 1:10000, 4°C, 18Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf