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alpha Tubulin antibody

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX52379

Cat. No. GTX52379

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all alpha Tubulin products

APPLICATION

Application Note

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Product Note

TUBA1A

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Tubulin alpha

Purification

Antigen affinity purification

Conjugation

Unconjugated

RRID

AB_2887799

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tubulin , alpha 1a Antibody , LIS3 Antibody , TUBA1A Antibody , TUBA3 Antibody , B-ALPHA-1 Antibody

Background

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition cha

Research Area

DATA IMAGES

Anti-alpha Tubulin antibody used in Western Blot (WB). GTX52379

GTX52379 WB Image

Western blot analysis of Tubulin alpha expression in NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

REFERENCE

Application Reference

Cheng CF et al. Pain 2015; 156 (11) : 2295-309 Nerve growth factor-induced synapse-like structures in contralateral sensory ganglia contribute to chronic mirror-image pain.
Application : WB
Reactivity : Rat
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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399