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beta 2 Adrenergic Receptor antibody [C2C3], C-term

Anti-beta 2 Adrenergic Receptor antibody [C2C3], C-term used in Western Blot (WB). GTX100040
Anti-beta 2 Adrenergic Receptor antibody [C2C3], C-term used in Western Blot (WB). GTX100040
Anti-beta 2 Adrenergic Receptor antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100040

Cat No. GTX100040

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

HepG2 , PC-12

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta 2 Adrenergic Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Adrenoceptor Beta 2,Adrb2R,Adrbr,B2Ar,Bar,Beta2Ar,Adrb2

Cellular Localization

Cell membrane

Background

This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This gene is intronless. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity and type 2 diabetes. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-beta 2 Adrenergic Receptor antibody [C2C3], C-term used in Western Blot (WB). GTX100040

GTX100040 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta 2 Adrenergic Receptor antibody [C2C3], C-term (GTX100040) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-beta 2 Adrenergic Receptor antibody [C2C3], C-term used in Western Blot (WB). GTX100040

GTX100040 WB Image

Sample (30 µg of whole cell lysate)
A: PC-12
10% SDS PAGE
GTX100040 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta 2 Adrenergic Receptor antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100040

GTX100040 IHC-P Image

beta 2 Adrenergic Receptor antibody [C2C3], C-term detects beta 2 Adrenergic Receptor protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: beta 2 Adrenergic Receptor antibody [C2C3], C-term (GTX100040) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf