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beta 2 Adrenergic Receptor (phospho Ser346) antibody

Orthogonal
Anti-beta 2 Adrenergic Receptor (phospho Ser346) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02869
Anti-beta 2 Adrenergic Receptor (phospho Ser346) antibody used in IHC (Paraffin sections) (IHC-P). GTX02869
Anti-beta 2 Adrenergic Receptor (phospho Ser346) antibody used in Western Blot (WB). GTX02869
Anti-beta 2 Adrenergic Receptor (phospho Ser346) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02869
Anti-beta 2 Adrenergic Receptor (phospho Ser346) antibody used in ELISA (ELISA). GTX02869
Anti-beta 2 Adrenergic Receptor (phospho Ser346) antibody used in IHC (Paraffin sections) (IHC-P). GTX02869

Cat. No. GTX02869

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all beta 2 Adrenergic Receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human ADRB2 around the phosphorylation site of Ser346.

Purification

Purified by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adrenoceptor beta 2 , ADRB2R , ADRBR , B2AR , BAR , BETA2AR

Cellular Localization

Cell membrane

Background

This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This gene is intronless. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity and type 2 diabetes. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-beta 2 Adrenergic Receptor (phospho Ser346) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02869

GTX02869 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX02869 beta 2 Adrenergic Receptor (phospho Ser346) antibody.
Permeabilization : 0.1% saponin
Dilution : 1:400

Anti-beta 2 Adrenergic Receptor (phospho Ser346) antibody used in IHC (Paraffin sections) (IHC-P). GTX02869

GTX02869 IHC-P Image

IHC-P analysis of mouse muscle tissue using GTX02869 beta 2 Adrenergic Receptor (phospho Ser346) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

Anti-beta 2 Adrenergic Receptor (phospho Ser346) antibody used in Western Blot (WB). GTX02869

GTX02869 WB Image

Orthogonal

WB analysis of nocodazole treated HepG2 whole cell lysates using GTX02869 beta 2 Adrenergic Receptor (phospho Ser346) antibody. -/+ means absence or presence of N peptide(non-phospho peptide) and P peptide(phospho peptide).

Anti-beta 2 Adrenergic Receptor (phospho Ser346) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02869

GTX02869 ICC/IF Image

ICC/IF analysis of PFA-fixed HUVEC cells using GTX02869 beta 2 Adrenergic Receptor (phospho Ser346) antibody.
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-beta 2 Adrenergic Receptor (phospho Ser346) antibody used in ELISA (ELISA). GTX02869

GTX02869 ELISA Image

Orthogonal

ELISA analysis of phospho- and non phosho- immunogen peptide using GTX02869 beta 2 Adrenergic Receptor (phospho Ser346) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml

Anti-beta 2 Adrenergic Receptor (phospho Ser346) antibody used in IHC (Paraffin sections) (IHC-P). GTX02869

GTX02869 IHC-P Image

IHC-P analysis of human brain tissue using GTX02869 beta 2 Adrenergic Receptor (phospho Ser346) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 399