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beta 2 Microglobulin antibody [B2M/1857R]

Anti-beta 2 Microglobulin antibody [B2M/1857R] used in Flow cytometry (FACS). GTX02596
Anti-beta 2 Microglobulin antibody [B2M/1857R] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02596
Anti-beta 2 Microglobulin antibody [B2M/1857R] used in Western Blot (WB). GTX02596
Anti-beta 2 Microglobulin antibody [B2M/1857R] used in IHC (Paraffin sections) (IHC-P). GTX02596

Cat No. GTX02596

Host

Rabbit

Clonality

Monoclonal

Clone Name

B2M/1857R

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

Human
Package
100 μg ($469)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-2 μg/ml
ICC/IF Assay dependent
IHC-P 1-2 μg/ml
FACS 1-2 μg/million cells
ELISA Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.

ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).

Not tested in other applications.

Calculated MW

14 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human B2M protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

beta-2-microglobulin , IMD43

Cellular Localization

Secreted

Background

This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.[provided by RefSeq, Aug 2014]

Database

Research Area

DATA IMAGES

Anti-beta 2 Microglobulin antibody [B2M/1857R] used in Flow cytometry (FACS). GTX02596

GTX02596 FACS Image

FACS analysis of HeLa cells using GTX02596 beta 2 Microglobulin antibody [B2M/1857R].
Blue : Primary antibody
Red : Isotype control

Anti-beta 2 Microglobulin antibody [B2M/1857R] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02596

GTX02596 ICC/IF Image

ICC/IF analysis of PFA fixed HeLa cells using GTX02596 beta 2 Microglobulin antibody [B2M/1857R].
Green : Primary antibody

Anti-beta 2 Microglobulin antibody [B2M/1857R] used in Western Blot (WB). GTX02596

GTX02596 WB Image

WB analysis of various samples using GTX02596 beta 2 Microglobulin antibody [B2M/1857R].
Lane 1 : THP-1 whole cell lysate
Lane 2 : Raji whole cell lysate

Anti-beta 2 Microglobulin antibody [B2M/1857R] used in IHC (Paraffin sections) (IHC-P). GTX02596

GTX02596 IHC-P Image

IHC-P analysis of human bladder carcinoma section using GTX02596 beta 2 Microglobulin antibody [B2M/1857R].

REFERENCE

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REVIEW

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Package List Price ($)
$ 469