beta 2 Microglobulin antibody [B2M/1857R]

Cat. No. GTX02596

Host	Rabbit
Clonality	Monoclonal
Clone Name	B2M/1857R
Isotype	IgG
Application	WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity	Human
Package	100 μg ($529)

See all beta 2 Microglobulin products

Datasheet

View product citations for antibody GTX02596 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2 μg/ml
ICC/IF	Assay dependent
IHC-P	1-2 μg/ml
FACS	1-2 μg/million cells
ELISA	Assay dependent
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes. ELISA For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).

Not tested in other applications.

Calculated MW

14 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human B2M protein

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

beta-2-microglobulin , IMD43

Cellular Localization

Secreted

Background

This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.[provided by RefSeq, Aug 2014]

Database

Gene ID: 567 B2M
UniProt: P61769 B2M

Research Area

DATA IMAGES

GTX02596 WB Image

WB analysis of various samples using GTX02596 beta 2 Microglobulin antibody [B2M/1857R].
Lane 1 : THP-1 whole cell lysate
Lane 2 : Raji whole cell lysate

GTX02596 ICC/IF Image

ICC/IF analysis of PFA fixed HeLa cells using GTX02596 beta 2 Microglobulin antibody [B2M/1857R].
Green : Primary antibody

GTX02596 IHC-P Image

IHC-P analysis of human bladder carcinoma section using GTX02596 beta 2 Microglobulin antibody [B2M/1857R].

GTX02596 FACS Image

FACS analysis of HeLa cells using GTX02596 beta 2 Microglobulin antibody [B2M/1857R].
Blue : Primary antibody
Red : Isotype control

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 529

Qty