beta 2 Microglobulin antibody (HRP)

Cat. No. GTX40576

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	250 μg ($399)

See all beta 2 Microglobulin products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:2,000
ICC/IF	Assay dependent
IHC-P	1:200 - 1:1,000

Not tested in other applications.

Calculated MW

14 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate pH7.2, 0.15M Sodium Chloride, 10mg/ml BSA

Preservative

0.01% Gentamicin Sulfate

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Anti-Beta-2-Microglobulin Antibody was produced by repeated immunizations with beta-2-Microglobulin protein isolated from human urine.

Purification

IgG fraction

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

beta-2-microglobulin , IMD43

Cellular Localization

Secreted

Background

Beta 2 Microglobulin non covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.

Database

Gene ID: 567 B2M
UniProt: P61769 B2M

Research Area

DATA IMAGES

GTX40576 WB Image

WB analysis using beta 2 Microglobulin antibody (HRP). Load: 50 ng Beta-2-Microglobulin. Predicted/Observed size: 12 kDa.

GTX40576 IHC-P Image

IHC-P analysis of human skin tissue using Isotype control (left) and beta 2 Microglobulin antibody (HRP) at a concentration of 1 ug/ml (right).

GTX40576 ICC/IF Image

ICC/IF analysis of 2% PFA fixed HEK293 cells using GTX40576 beta 2 Microglobulin antibody (HRP).
Dilution : 1:200
Secondary antibody : donkey anti-rabbit secondary antibody conjugated with Alexa Fluor 488.

REFERENCE

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REVIEW

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Package

List Price ($)

250 μg

$ 399

Qty