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beta 2 Microglobulin antibody (HRP)

Anti-beta 2 Microglobulin antibody (HRP) used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX40576
Anti-beta 2 Microglobulin antibody (HRP) used in Western Blot (WB). GTX40576
Anti-beta 2 Microglobulin antibody (HRP) used in IHC (Paraffin sections) (IHC-P). GTX40576

Cat No. GTX40576

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
250 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2,000
ICC/IF Assay dependent
IHC-P 1:200 - 1:1,000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate pH7.2, 0.15M Sodium Chloride, 10mg/ml BSA

Preservative

0.01% Gentamicin Sulfate

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Anti-Beta-2-Microglobulin Antibody was produced by repeated immunizations with beta-2-Microglobulin protein isolated from human urine.

Purification

IgG fraction

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

beta-2-microglobulin , IMD43

Cellular Localization

Secreted

Background

Beta 2 Microglobulin non covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.

Database

Research Area

DATA IMAGES

Anti-beta 2 Microglobulin antibody (HRP) used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX40576

GTX40576 ICC/IF Image

ICC/IF analysis of 2% PFA fixed HEK293 cells using GTX40576 beta 2 Microglobulin antibody (HRP).
Dilution : 1:200
Secondary antibody : donkey anti-rabbit secondary antibody conjugated with Alexa Fluor 488.

Anti-beta 2 Microglobulin antibody (HRP) used in Western Blot (WB). GTX40576

GTX40576 WB Image

WB analysis using beta 2 Microglobulin antibody (HRP). Load: 50 ng Beta-2-Microglobulin. Predicted/Observed size: 12 kDa.

Anti-beta 2 Microglobulin antibody (HRP) used in IHC (Paraffin sections) (IHC-P). GTX40576

GTX40576 IHC-P Image

IHC-P analysis of human skin tissue using Isotype control (left) and beta 2 Microglobulin antibody (HRP) at a concentration of 1 ug/ml (right).

REFERENCE

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REVIEW

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Package List Price ($)
$ 379