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beta 2 Microglobulin antibody [N1C3]

KO/KD
Anti-beta 2 Microglobulin antibody [N1C3] used in Western Blot (WB). GTX101314
Anti-beta 2 Microglobulin antibody [N1C3] used in Western Blot (WB). GTX101314
Anti-beta 2 Microglobulin antibody [N1C3] used in Western Blot (WB). GTX101314

Cat No. GTX101314

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Rat
Package
100 μl ($329),
25 μl ($169)
See all beta 2 Microglobulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , Raji , rat spleen , B2M shRNA-transfected HeLa

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.409 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta 2 Microglobulin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

beta-2-microglobulin , IMD43

Cellular Localization

Secreted

Background

Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells (Gussow et al., 1987 [PubMed 3312414]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-beta 2 Microglobulin antibody [N1C3] used in Western Blot (WB). GTX101314

GTX101314 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody [N1C3] (GTX101314) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta 2 Microglobulin antibody [N1C3] used in Western Blot (WB). GTX101314

GTX101314 WB Image

Sample(30 μg of whole cell lysate)
A:Raji (GTX27908)
15% SDS PAGE
GTX101314 diluted at 1:500

Anti-beta 2 Microglobulin antibody [N1C3] used in Western Blot (WB). GTX101314

GTX101314 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody [N1C3] (GTX101314) diluted at 1:500.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169