beta 2 Microglobulin antibody [SPM617]

Cat. No. GTX34436

Host	Mouse
Clonality	Monoclonal
Clone Name	SPM617
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human, Primate
Package	100 μg ($529)

See all beta 2 Microglobulin products

Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2μg/ml
ICC/IF	1-2μg/ml
IHC-P	1-2μg/ml for 30 minutes at RT
FACS	1-2μg/10⁶ cells
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

14 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length recombinant human B2M protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

B2M , IMD43 , beta2microglobulin , beta 2 Microglobulin , b2m , beta-2-microglobulin

Cellular Localization

Secreted,Cell surface

Background

This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.[provided by RefSeq, Aug 2014]

Database

Gene ID: 567 B2M
UniProt: P61769 B2M

Research Area

DATA IMAGES

GTX34436 IHC-P Image

IHC-P analysis of human cervical carcinoma tissue using GTX34436 beta 2 Microglobulin antibody [SPM617].

GTX34436 WB Image

WB analysis of THP1 and Raji cell lysates using GTX34436 beta 2 Microglobulin antibody [SPM617].

GTX34436 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX34436 beta 2 Microglobulin antibody [SPM617].
Green : Primary antibody

GTX34436 FACS Image

FACS analysis of PFA-fixed HeLa cells using GTX34436 beta 2 Microglobulin antibody [SPM617].
Blue : Primary antibody
Red : Isotype control

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 529

Qty