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beta 2 Microglobulin antibody

Anti-beta 2 Microglobulin antibody used in Western Blot (WB). GTX112815
Anti-beta 2 Microglobulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112815
Anti-beta 2 Microglobulin antibody used in Western Blot (WB). GTX112815
Anti-beta 2 Microglobulin antibody used in Immunoprecipitation (IP). GTX112815
Anti-beta 2 Microglobulin antibody used in Western Blot (WB). GTX112815
Anti-beta 2 Microglobulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112815
Anti-beta 2 Microglobulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112815
Anti-beta 2 Microglobulin antibody used in Western Blot (WB). GTX112815
Anti-beta 2 Microglobulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112815

Cat No. GTX112815

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat, Marmoset
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

A431 , mouse plasma , rat plasma , HeLa , B2M shRNA-transfected HeLa , *marmoset rProtein

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human beta 2 Microglobulin Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175135

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

beta-2-microglobulin , IMD43

Cellular Localization

Secreted

Background

Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells (Gussow et al., 1987 [PubMed 3312414]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-beta 2 Microglobulin antibody used in Western Blot (WB). GTX112815

GTX112815 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody (GTX112815) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta 2 Microglobulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112815

GTX112815 ICC/IF Image

beta 2 Microglobulin antibody detects beta 2 Microglobulin protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta 2 Microglobulin stained by beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-beta 2 Microglobulin antibody used in Western Blot (WB). GTX112815

GTX112815 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.

Anti-beta 2 Microglobulin antibody used in Immunoprecipitation (IP). GTX112815

GTX112815 IP Image

beta 2 Microglobulin antibody immunoprecipitates B2M protein in IP experiments.
IP samples: HeLa whole cell extract
A. 30 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of B2M protein by 4 μg beta 2 Microglobulin antibody (GTX112815)
15 % SDS-PAGE
The immunoprecipitated B2M protein was detected by beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-beta 2 Microglobulin antibody used in Western Blot (WB). GTX112815

GTX112815 WB Image

Sample (30 ug of whole cell lysate)
A: A431
15% SDS PAGE
GTX112815 diluted at 1:5000

Anti-beta 2 Microglobulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112815

GTX112815 IHC-P Image

beta 2 Microglobulin antibody detects B2M protein at cytosol on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
beta 2 Microglobulin antibody (GTX112815) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-beta 2 Microglobulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112815

GTX112815 IHC-P Image

beta 2 Microglobulin antibody detects beta 2 Microglobulin protein at cytoplasm in human endometrial cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial cancer.
beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta 2 Microglobulin antibody used in Western Blot (WB). GTX112815

GTX112815 WB Image

Mouse plasma (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody (GTX112815) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta 2 Microglobulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112815

GTX112815 IHC-P Image

Beta 2 Microglobulin antibody detects secreted B2M protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
B2M stained by Beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

beta 2 Microglobulin antibody Cat No. GTX112815

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 05-Jun-2014
Score :
Application Tested : WB
Sample Species : Mrt
Sample : marmoset rProtein
Amount used : 5μg
Blocking : skim milk, 25°C, 2Hr
Primary Antibody : 1:1000, 25°C, 2Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169