A431 , mouse plasma , rat plasma , HeLa , B2M shRNA-transfected HeLa , *marmoset rProtein
Predict Reactivity
Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.83 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human beta 2 Microglobulin Recombinant protein.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11175135
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
beta-2-microglobulin , IMD43
Cellular Localization
Secreted
Background
Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells (Gussow et al., 1987 [PubMed 3312414]).[supplied by OMIM]
Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody (GTX112815) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112815 WB Image
Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.
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GTX112815 IP Image
beta 2 Microglobulin antibody immunoprecipitates B2M protein in IP experiments. IP samples: HeLa whole cell extract A. 30 μg HeLa whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of B2M protein by 4 μg beta 2 Microglobulin antibody (GTX112815) 15 % SDS-PAGE The immunoprecipitated B2M protein was detected by beta 2 Microglobulin antibody (GTX112815) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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GTX112815 WB Image
Sample (30 ug of whole cell lysate) A: A431 15% SDS PAGE GTX112815 diluted at 1:5000
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GTX112815 IHC-P Image
beta 2 Microglobulin antibody detects B2M protein at cytosol on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. beta 2 Microglobulin antibody (GTX112815) dilution: 1:500.
beta 2 Microglobulin antibody detects beta 2 Microglobulin protein at cytoplasm in human endometrial cancer by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial cancer. beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX112815 IHC-P Image
Beta 2 Microglobulin antibody detects secreted B2M protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse spleen.B2M stained by Beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX112815 WB Image
Mouse plasma (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody (GTX112815) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX112815 ICC/IF Image
beta 2 Microglobulin antibody detects beta 2 Microglobulin protein by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: beta 2 Microglobulin stained by beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
