beta 2 Microglobulin antibody

Name: beta 2 Microglobulin antibody
Brand: GeneTex
SKU: GTX112815
Price: 399.00 USD
Rating: 4.5 (2 reviews)

Anti-beta 2 Microglobulin antibody used in Western Blot (WB). GTX112815

Anti-beta 2 Microglobulin antibody used in Immunoprecipitation (IP). GTX112815

Anti-beta 2 Microglobulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112815

Anti-beta 2 Microglobulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112815

Cat. No. GTX112815

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat, Marmoset
Package	100 μl ($399), 25 μl ($169)

See all beta 2 Microglobulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

A431 , rat plasma , HeLa

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human beta 2 Microglobulin Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175135

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

beta-2-microglobulin , IMD43

Cellular Localization

Secreted

Background

Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells (Gussow et al., 1987 [PubMed 3312414]).[supplied by OMIM]

Database

Gene ID: 567 B2M
UniProt: P61769 B2M

Research Area

DATA IMAGES

GTX112815 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody (GTX112815) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112815 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.

GTX112815 IP Image

beta 2 Microglobulin antibody immunoprecipitates B2M protein in IP experiments.
IP samples: HeLa whole cell extract
A. 30 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of B2M protein by 4 μg beta 2 Microglobulin antibody (GTX112815)
15 % SDS-PAGE
The immunoprecipitated B2M protein was detected by beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX112815 WB Image

Sample (30 ug of whole cell lysate)
A: A431
15% SDS PAGE
GTX112815 diluted at 1:5000

GTX112815 IHC-P Image

beta 2 Microglobulin antibody detects B2M protein at cytosol on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
beta 2 Microglobulin antibody (GTX112815) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX112815 IHC-P Image

beta 2 Microglobulin antibody detects beta 2 Microglobulin protein at cytoplasm in human endometrial cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial cancer.
beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112815 WB Image

Mouse plasma (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody (GTX112815) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112815 ICC/IF Image

beta 2 Microglobulin antibody detects beta 2 Microglobulin protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta 2 Microglobulin stained by beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX112815 IHC-P Image

Beta 2 Microglobulin antibody detects secreted B2M protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
B2M stained by Beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Susukida T et al. Arch Toxicol 2017; (Epub) Evaluation of immune-mediated idiosyncratic drug toxicity using chimeric HLA transgenic mice.

Application : WB

Reactivity : Human

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REVIEW