beta 2 Microglobulin antibody

Name: beta 2 Microglobulin antibody
Brand: GeneTex
SKU: GTX112815
Price: 399.00 USD
Rating: 4.5 (2 reviews)

Anti-beta 2 Microglobulin antibody used in Western Blot (WB). GTX112815

Anti-beta 2 Microglobulin antibody used in IHC (Paraffin sections) (IHC-P). GTX112815

Anti-beta 2 Microglobulin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112815

Anti-beta 2 Microglobulin antibody used in Immunoprecipitation (IP). GTX112815

Cat. No. GTX112815

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP
Reactivity	Human, Mouse, Rat, Marmoset
Package	100 μl ($399), 25 μl ($169)

See all beta 2 Microglobulin products

View product citations for antibody GTX112815 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

A431 , rat plasma , HeLa

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.37 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human beta 2 Microglobulin Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175135

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

beta-2-microglobulin , IMD43

Cellular Localization

Secreted

Background

Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells (Gussow et al., 1987 [PubMed 3312414]).[supplied by OMIM]

Database

Gene ID: 567 B2M
UniProt: P61769 B2M

Research Area

DATA IMAGES

GTX112815 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody (GTX112815) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112815 IHC-P Image

beta 2 Microglobulin antibody detects B2M protein at cytosol on Cal27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Cal27 xenograft.
beta 2 Microglobulin antibody (GTX112815) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX112815 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.

GTX112815 WB Image

Mouse plasma (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody (GTX112815) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112815 IHC-P Image

beta 2 Microglobulin antibody detects beta 2 Microglobulin protein at cytoplasm in human endometrial cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial cancer.
beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112815 IHC-P Image

Beta 2 Microglobulin antibody detects secreted B2M protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
B2M stained by Beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112815 ICC/IF Image

beta 2 Microglobulin antibody detects beta 2 Microglobulin protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta 2 Microglobulin stained by beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX112815 IP Image

beta 2 Microglobulin antibody immunoprecipitates B2M protein in IP experiments.
IP samples: HeLa whole cell extract
A. 30 μg HeLa whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of B2M protein by 4 μg beta 2 Microglobulin antibody (GTX112815)
15 % SDS-PAGE
The immunoprecipitated B2M protein was detected by beta 2 Microglobulin antibody (GTX112815) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

GTX112815 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody (GTX112815) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Susukida T et al. Arch Toxicol 2017; (Epub) Evaluation of immune-mediated idiosyncratic drug toxicity using chimeric HLA transgenic mice.

Application : WB

Reactivity : Human

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