APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Mouse brain , 293T , A431 , HeLa , HepG2 , H1299
Predict Reactivity
Rat, Bovine, Dog, Guinea pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human beta A4 Crystallin. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950043
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
crystallin beta A4 , CTRCT23 , CYRBA4 , MCOPCT4
Background
Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta acidic group member, is part of a gene cluster with beta-B1, beta-B2, and beta-B3. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-beta A4 Crystallin antibody [N1C3-2] used in Western Blot (WB). GTX109526](/templates/default/js/lightbox/images/loading.gif "Anti-beta A4 Crystallin antibody [N1C3-2] used in Western Blot (WB). GTX109526")
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GTX109526 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with beta A4 Crystallin antibody [N1C3-2] (GTX109526) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX109526 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Beta-crystallin A4(GTX109526) antibody at 1:200 dilution.
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GTX109526 WB Image
Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX109526 diluted at 1:1000
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GTX109526 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with beta A4 Crystallin antibody [N1C3-2] (GTX109526) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for beta A4 Crystallin antibody [N1C3-2] (GTX109526). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for beta A4 Crystallin antibody [N1C3-2] (GTX109526). Be the first to share your experience with this product.
![Anti-beta A4 Crystallin antibody [N1C3-2] used in Western Blot (WB). GTX109526](/upload/website/prouct_img/small/GTX109526/GTX109526_40023_20170825_WB_2_w_23060500_529.webp "Anti-beta A4 Crystallin antibody [N1C3-2] used in Western Blot (WB). GTX109526")
![Anti-beta A4 Crystallin antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109526](/upload/website/prouct_img/small/GTX109526/GTX109526_IFA_w_23060500_264.webp "Anti-beta A4 Crystallin antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109526")
![Anti-beta A4 Crystallin antibody [N1C3-2] used in Western Blot (WB). GTX109526](/upload/website/prouct_img/small/GTX109526/GTX109526_40023_WB_M_brain_w_23060500_498.webp "Anti-beta A4 Crystallin antibody [N1C3-2] used in Western Blot (WB). GTX109526")
![Anti-beta A4 Crystallin antibody [N1C3-2] used in Western Blot (WB). GTX109526](/upload/website/prouct_img/small/GTX109526/GTX109526_40023_20170825_WB_w_23060500_366.webp "Anti-beta A4 Crystallin antibody [N1C3-2] used in Western Blot (WB). GTX109526")
![Anti-beta A4 Crystallin antibody [N1C3-2] used in Western Blot (WB). GTX109526](/upload/website/prouct_img/normal/GTX109526/GTX109526_40023_20170825_WB_2_w_23060500_529.webp "Anti-beta A4 Crystallin antibody [N1C3-2] used in Western Blot (WB). GTX109526")
![Anti-beta A4 Crystallin antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109526](/upload/website/prouct_img/normal/GTX109526/GTX109526_IFA_w_23060500_264.webp "Anti-beta A4 Crystallin antibody [N1C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109526")
![Anti-beta A4 Crystallin antibody [N1C3-2] used in Western Blot (WB). GTX109526](/upload/website/prouct_img/normal/GTX109526/GTX109526_40023_WB_M_brain_w_23060500_498.webp "Anti-beta A4 Crystallin antibody [N1C3-2] used in Western Blot (WB). GTX109526")
![Anti-beta A4 Crystallin antibody [N1C3-2] used in Western Blot (WB). GTX109526](/upload/website/prouct_img/normal/GTX109526/GTX109526_40023_20170825_WB_w_23060500_366.webp "Anti-beta A4 Crystallin antibody [N1C3-2] used in Western Blot (WB). GTX109526")