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beta Actin antibody [8H10D10]

Anti-beta Actin antibody [8H10D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83163
Anti-beta Actin antibody [8H10D10] used in Flow cytometry (FACS). GTX83163
Anti-beta Actin antibody [8H10D10] used in Western Blot (WB). GTX83163

Cat. No. GTX83163

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8H10D10

Isotype

 IgG2b

Application

 WB, ICC/IF, FACS, ELISA

Reactivity

 Human, Mouse, Rat, Hamster, Monkey
Package
100 μl ($399)
See all beta Actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to amino-terminal residues of human beta-Actin, conjugated to KLH.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_11162633

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin beta , BRWS1 , PS1TP5BP1

Cellular Localization

Cytoplasm, cytoskeleton,Nucleus

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-beta Actin antibody [8H10D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83163

GTX83163 ICC/IF Image

ICC/IF analysis of SKBR-3 (left) and A549 (right) cells using GTX83163 beta Actin antibody [8H10D10].
Red : beta Actin
Blue: DRAQ5 fluorescent DNA dye

Anti-beta Actin antibody [8H10D10] used in Flow cytometry (FACS). GTX83163

GTX83163 FACS Image

FACS analysis of MCF-7 cells using GTX83163 beta Actin antibody [8H10D10].
Right : beta Actin
Left : negative control

Anti-beta Actin antibody [8H10D10] used in Western Blot (WB). GTX83163

GTX83163 WB Image

WB analysis of NIH3T3 (1), Jurkat (2), HeLa (3), CHO (4), PC-12 (5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysate using GTX83163 beta Actin antibody [8H10D10].

REFERENCE

Application Reference

Duan L et al. Oncol Rep 2019; 41 (4) : 2379-2388 The role of GDF15 in bone metastasis of lung adenocarcinoma cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399