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beta Actin antibody [AC-15]

Anti-beta Actin antibody [AC-15] used in IHC (Paraffin sections) (IHC-P). GTX26276
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Anti-beta Actin antibody [AC-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26276
Anti-beta Actin antibody [AC-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26276

Cat No. GTX26276

Host

Mouse

Clonality

Monoclonal

Clone Name

AC-15

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IP, ELISA

Reactivity

Human, Mouse, Rat, Rabbit, Sheep, Bovine, Cat, Dog, Chicken, Guinea pig, Pig, Monkey, Carp, Hirudo medicinalis
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5,000-1:10,000
ICC/IF 1:1,000-1:2,000
IHC-P Assay dependent
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

human or chicken fibroblast

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Xenopus laevis

Immunogen

slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_367161

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

actin beta , BRWS1 , PS1TP5BP1

Cellular Localization

Cytoplasm, cytoskeleton,Nucleus

Background

Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity.

Database

Research Area

DATA IMAGES

Anti-beta Actin antibody [AC-15] used in IHC (Paraffin sections) (IHC-P). GTX26276

GTX26276 IHC-P Image

IHC-P analysis of human heart tissue using GTX26276 beta Actin antibody [AC-15] at 1:1,000.

Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276

GTX26276 WB Image

WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK and (9) MDCK lysates using beta Actin antibody [AC-15] at 1:5,000.

Anti-beta Actin antibody [AC-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26276

GTX26276 ICC/IF Image

ICC/IF analysis of FS-11 cells using GTX26276 beta Actin antibody [AC-15].

Anti-beta Actin antibody [AC-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26276

GTX26276 ICC/IF Image

ICC/IF analysis of HS-68 cells using GTX26276 beta Actin antibody [AC-15] at 1:1000 (green) with DAPI (blue). Cells were fixed and permeabilized with methanol followed by methanol:acetone.

REFERENCE

REVIEW

beta Actin antibody [AC-15] Cat No. GTX26276

Rating ( Average 5 based on 17 users reviews)
Application
Western Blot(WB) ( Average 5 based on 14 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 3 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Allergy Clin Immunol in 2017. PMID: 27913303
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal J Allergy Clin Immunol in 2017. PMID: 27913303
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Allergy Clin Immunol in 2017. PMID: 27913303
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Allergy Clin Immunol in 2017. PMID: 27913303
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal J Allergy Clin Immunol in 2017. PMID: 27913303
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal J Allergy Clin Immunol in 2017. PMID: 27913303
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nucleic Acids Res in 2014. PMID: 24589584
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nucleic Acids Res in 2014. PMID: 24589584
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Biomed Sci in 2015. PMID: 25981436
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2019. PMID: 30787373
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Glycoconj J in 2015. PMID: 26104834
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018. PMID: 29691463
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal J Neurosci in 2017. PMID: 28724750
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Biomedicines in 2020. PMID: 32244938
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2019. PMID: 31480758
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Front Oncol in 2019. PMID: 31448224
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016. PMID: 27848974
Application Reference
SDS
Sodium Azide.pdf
Package List Price ($)
$ 339