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beta Actin antibody [AC-15]

KO/KD
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Anti-beta Actin antibody [AC-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26276
Anti-beta Actin antibody [AC-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26276
Anti-beta Actin antibody [AC-15] used in IHC (Paraffin sections) (IHC-P). GTX26276

Cat. No. GTX26276

Reference 54
17

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AC-15

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP, ELISA

Reactivity

 Human, Mouse, Rat, Rabbit, Sheep, Bovine, Cat, Dog, Chicken, Guinea pig, Pig, Monkey, Carp, Hirudo medicinalis
Package
100 μl ($399)
See all beta Actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5,000-1:10,000
ICC/IF 1:1,000-1:2,000
IHC-P Assay dependent
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

human or chicken fibroblast

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Xenopus laevis

Immunogen

slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_367161

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

actin beta , BRWS1 , PS1TP5BP1

Cellular Localization

Cytoplasm, cytoskeleton,Nucleus

Background

Beta actin is one of six different actin isoforms which have been identified. Actins are highly conserved proteins that are involved in cell motility, structure and integrity.

Database

Research Area

DATA IMAGES

Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276

GTX26276 WB Image

WB analysis of (1) HeLa, (2) Jurkat, (3) COS7, (4) NIH3T3, (5) PC-12, (6) RAT2, (7) CHO, (8) MDBK and (9) MDCK lysates using beta Actin antibody [AC-15] at 1:5,000.

Anti-beta Actin antibody [AC-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26276

GTX26276 ICC/IF Image

ICC/IF analysis of FS-11 cells using GTX26276 beta Actin antibody [AC-15].

Anti-beta Actin antibody [AC-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26276

GTX26276 ICC/IF Image

ICC/IF analysis of HS-68 cells using GTX26276 beta Actin antibody [AC-15] at 1:1000 (green) with DAPI (blue). Cells were fixed and permeabilized with methanol followed by methanol:acetone.

Anti-beta Actin antibody [AC-15] used in IHC (Paraffin sections) (IHC-P). GTX26276

GTX26276 IHC-P Image

IHC-P analysis of human heart tissue using GTX26276 beta Actin antibody [AC-15] at 1:1,000.

REFERENCE

Application Reference

Klampfleuthner FAM et al. Cells 2022; 11 (19) Stage-Dependent Activity and Pro-Chondrogenic Function of PI3K/AKT during Cartilage Neogenesis from Mesenchymal Stromal Cells.
Jessica Giehrl-Schwab et al. EMBO Mol Med 2022; 14 (5) : e14797 Parkinson's disease motor symptoms rescue by CRISPRa-reprogramming astrocytes into GABAergic neurons.
Application : WB
Janine L?ckgen et al. Stem Cell Res Ther 2022; 13 (1) : 168 NFκB inhibition to lift the mechano-competence of mesenchymal stromal cell-derived neocartilage toward articular chondrocyte levels.
Application : WB
Matthias Gerstner et al. Int J Mol Sci 2021; 22 (7) Heparan Sulfate Deficiency in Cartilage: Enhanced BMP-Sensitivity, Proteoglycan Production and an Anti-Apoptotic Expression Signature after Loading.
Application : WB
Reactivity : Mouse
Yanjun Liu et al. Redox Biol 2021; 43 : 102002 Plasmalogen attenuates the development of hepatic steatosis and cognitive deficit through mechanism involving p75NTR inhibition.
Application : WB
Reactivity : Mouse
Show More +
Submit a Reference

REVIEW

beta Actin antibody [AC-15] Cat. No. GTX26276

Rating
( Average 5 based on 17 users reviews)
Application
Western Blot(WB)
( Average 5 based on 14 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 3 users reviews)
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cancer Cell Int in 2016.PMID: 27418879
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2016.PMID: 27876799
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2019.PMID: 31640125
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2019.PMID: 31640125
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Front Mol Neurosci in 2018.PMID: 29910711
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2013.PMID: 23675505
Anti-beta Actin antibody [AC-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26276
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Front Mol Neurosci in 2017.PMID: 29163025
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2019.PMID: 31640125
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2019.PMID: 31640125
Anti-beta Actin antibody [AC-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26276
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Front Mol Neurosci in 2017.PMID: 29163025
Anti-beta Actin antibody [AC-15] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26276
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Front Mol Neurosci in 2017.PMID: 29163025
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2018. PMID: 29691463
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal J Neurosci in 2017. PMID: 28724750
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Biomedicines in 2020. PMID: 32244938
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2019. PMID: 31480758
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Front Oncol in 2019. PMID: 31448224
Anti-beta Actin antibody [AC-15] used in Western Blot (WB). GTX26276
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016. PMID: 27848974
Show More +
Submit a Review
Application Reference
Klampfleuthner FAM et al. Cells 2022; 11 (19) Stage-Dependent Activity and Pro-Chondrogenic Function of PI3K/AKT during Cartilage Neogenesis from Mesenchymal Stromal Cells.
Jessica Giehrl-Schwab et al. EMBO Mol Med 2022; 14 (5):e14797 Parkinson's disease motor symptoms rescue by CRISPRa-reprogramming astrocytes into GABAergic neurons.
Application : WB
Janine L?ckgen et al. Stem Cell Res Ther 2022; 13 (1):168 NFκB inhibition to lift the mechano-competence of mesenchymal stromal cell-derived neocartilage toward articular chondrocyte levels.
Application : WB
Matthias Gerstner et al. Int J Mol Sci 2021; 22 (7) Heparan Sulfate Deficiency in Cartilage: Enhanced BMP-Sensitivity, Proteoglycan Production and an Anti-Apoptotic Expression Signature after Loading.
Application : WB
Reactivity : Mouse
Yanjun Liu et al. Redox Biol 2021; 43:102002 Plasmalogen attenuates the development of hepatic steatosis and cognitive deficit through mechanism involving p75NTR inhibition.
Application : WB
Reactivity : Mouse
Chasan S et al. Advanced Functional Materials 2021; (Epub) Sulfation of Glycosaminoglycan Hydrogels Instructs Cell Fate and Chondral versus Endochondral Lineage Decision of Skeletal Stem Cells In Vivo.
Application : WB
Reactivity : Human
Ghanawi H et al. Nucleic Acids Res 2021; 49 (21):12284-12305 Loss of full-length hnRNP R isoform impairs DNA damage response in motoneurons by inhibiting Yb1 recruitment to chromatin.
Application : WB
Reactivity : Mouse
Raman V et al. Nat Commun 2021; 12 (1):6116 Intracellular delivery of protein drugs with an autonomously lysing bacterial system reduces tumor growth and metastases.
Application : WB
Teng YN et al. Cells 2021; 10 (6) Etoposide Triggers Cellular Senescence by Inducing Multiple Centrosomes and Primary Cilia in Adrenocortical Tumor Cells.
Application : WB
Reactivity : Mouse
Chen TY et al. Cell Death Differ 2021; (Epub) Genotoxic stress-activated DNA-PK-p53 cascade and autophagy cooperatively induce ciliogenesis to maintain the DNA damage response.
Application : WB
Reactivity : Human
Vikram Babu Kasaragod et al. Proc Natl Acad Sci U S A 2020; 117 (52):33235-33245 Pyridoxal kinase inhibition by artemisinins down-regulates inhibitory neurotransmission.
Application : WB
Reactivity : Human
Tanaka K et al. PLoS One 2020; 15 (9):e0238604 Difference in pyruvic acid metabolism between neonatal and adult mouse lungs exposed to hyperoxia.
Melnik S et al. Stem Cell Res Ther 2020; 11 (1):532 MiR-218 affects hypertrophic differentiation of human mesenchymal stromal cells during chondrogenesis via targeting RUNX2, MEF2C, and COL10A1.
Application : WB
Reactivity : Human
Dreher SI et al. Front Cell Dev Biol 2020; 8:81 Significance of MEF2C and RUNX3 Regulation for Endochondral Differentiation of Human Mesenchymal Progenitor Cells.
Kreuser U et al. Front Cell Dev Biol 2020; 8:581331 Initial WNT/β-Catenin Activation Enhanced Mesoderm Commitment, Extracellular Matrix Expression, Cell Aggregation and Cartilage Tissue Yield From Induced Pluripotent Stem Cells.
Application : WB
Reactivity : Human
Vikram Babu Kasaragod et al. bioRxiv 2020; (Epub) Pyridoxal Kinase Inhibition by Artemisinins Downregulates Inhibitory Neurotransmission.
Application : WB
Nagano N et al. Biomedicines 2020; 8 (3) Attenuation of Hyperoxic Lung Injury in Newborn Thioredoxin-1-Overexpressing Mice through the Suppression of Proinflammatory Cytokine mRNA Expression.
Application : IP
Reactivity : Mouse
Saha S et al. Front Oncol 2019; 9:692 Transcriptomic Analysis Identifies RNA Binding Proteins as Putative Regulators of Myelopoiesis and Leukemia.
Application : WB
Reactivity : Human
Wang CY et al. Int J Mol Sci 2019; 20 (20) Fetuin-A Inhibits Placental Cell Growth and Ciliogenesis in Gestational Diabetes Mellitus.
Application : WB
Reactivity : Human
Buchert J et al. Int J Mol Sci 2019; 20 (17) The Role of Extracellular Matrix Expression, ERK1/2 Signaling and Cell Cohesiveness for Cartilage Yield from iPSCs.
Application : WB
Reactivity : Human
Claes Z et al. J Biol Chem 2019; The antibiotic robenidine exhibits guanabenz-like cytoprotective properties by a mechanism independent of protein phosphatase PP1:PPP1R51A.
Application : WB
Reactivity : Human
Diederichs S et al. Cell Mol Life Sci 2019; (Epub) Regulation of WNT5A and WNT11 during MSC in vitro chondrogenesis: WNT inhibition lowers BMP and hedgehog activity, and reduces hypertrophy.
Application : WB
Reactivity : Mouse
Colussi DJ et al. SLAS Discov 2019; 24 (3):295-303 Patient-Derived Phenotypic High-Throughput Assay to Identify Small Molecules Restoring Lysosomal Function in Tay-Sachs Disease.
Application : WB
Reactivity : Human
Schaefer N et al. Front Mol Neurosci 2018; 11:167 Functional Consequences of the Postnatal Switch From Neonatal to Mutant Adult Glycine Receptor α1 Subunits in the Shaky Mouse Model of Startle Disease.
Chen TY et al. J Cell Physiol 2018; (Epub) Chloroquine inhibits human retina pigmented epithelial cell growth and microtubule nucleation by downregulating p150glued.
Application : WB
Reactivity : Human
Bhattacharjee P et al. Sci Rep 2018; 8 (1):6458 Cholesterol-dependent cytolysins impair pro-inflammatory macrophage responses.
Application : WB
Reactivity : Mouse
Romero M et al. Cell Death Differ 2017; 24 (5):798-808 Intrinsic repair protects cells from pore-forming toxins by microvesicle shedding.
Dombert B et al. Front Mol Neurosci 2017; 10:346 BDNF/trkB Induction of Calcium Transients through Cav2.2 Calcium Channels in Motoneurons Corresponds to F-actin Assembly and Growth Cone Formation on β2-Chain Laminin (221).
Application : ICC/IF
Reactivity : Mouse
Shan YS et al. Oncol Lett 2017; 14 (4):4517-4526 Cyclin D1 overexpression correlates with poor tumor differentiation and prognosis in gastric cancer.
Moradi M et al. J Cell Biol 2017; 216 (3):793-814 Differential roles of α-, β-, and γ-actin in axon growth and collateral branch formation in motoneurons.
Lin RK et al. Oncotarget 2017; 8 (69):113431-113443 Hypermethylation of BEND5 contributes to cell proliferation and is a prognostic marker of colorectal cancer.
Application : WB
Reactivity : Human
Shan YS et al. Oncol Lett 2017; (Epub) Cyclin D1 overexpression correlates with poor tumor differentiation and prognosis in gastric cancer.
Application : WB
Reactivity : Human
Schaefer N et al. J Neurosci 2017; 37 (33):7948-7961 Disruption of a Structurally Important Extracellular Element in the Glycine Receptor Leads to Decreased Synaptic Integration and Signaling Resulting in Severe Startle Disease.
Application : WB
Reactivity : Mouse
He C et al. Prostate 2017; 77 (11):1187-1198 Characterization of a novel p110β-specific inhibitor BL140 that overcomes MDV3100-resistance in castration-resistant prostate cancer cells.
Application : WB
Reactivity : Human
Mohammadreza Zamanian et al. Cancer Cell Int 2016; 16:56 Calreticulin mediates an invasive breast cancer phenotype through the transcriptional dysregulation of p53 and MAPK pathways.
Application : WB
Reactivity : Human
Ikink GJ et al. Nat Commun 2016; 7:13567 IRS4 induces mammary tumorigenesis and confers resistance to HER2-targeted therapy through constitutive PI3K/AKT-pathway hyperactivation.
Application : WB
Reactivity : Human
Dexheimer V et al. Sci Rep 2016; 6:36655 Differential expression of TGF-β superfamily members and role of Smad1/5/9-signalling in chondral versus endochondral chondrocyte differentiation.
Application : WB
Reactivity : Human
Fischer J et al. JCP 2016; (Epub) Role of PTHrP(1-34) Pulse Frequency Versus Pulse Duration to Enhance Mesenchymal Stromal Cell Chondrogenesis.
Application : WB
Reactivity : Human
Tseng YY et al. J Virol 2015; 89 (22):11619-29 Regulation of PACT-Mediated Protein Kinase Activation by the OV20.0 Protein of Orf Virus.
Bramanti V et al. J Neurosci Res 2015; 93 (9):1378-87 modulation of extracellular signal-related kinase, cyclin d1, glial fibrillary acidic protein, and vimentin expression in *-pretreated astrocyte cultures treated with competence and progression growth factors.
Application : WB
Reactivity : Rat
Hamacher-Brady A et al. J Biol Chem 2015; 290 (36):22005-18 Bax/Bak-dependent, Drp1-independent Targeting of X-linked Inhibitor of Apoptosis Protein (XIAP) into Inner Mitochondrial Compartments Counteracts Smac/DIABLO-dependent Effector Caspase Activation.
Application : WB
Reactivity : Human
Tseng YY et al. J Virol 2015; 89 (9):4966-79 Functional analysis of the short isoform of orf virus protein OV20.0.
Application : WB
Reactivity : Human
Hamacher-Brady A et al. Cell Death Differ 2014; 21 (12):1862-76 Intramitochondrial recruitment of endolysosomes mediates Smac degradation and constitutes a novel intrinsic apoptosis antagonizing function of XIAP E3 ligase.
Hou HH et al. Autophagy 2014; 10 (9):1509-21 Elastase induces lung epithelial cell autophagy through placental growth factor: a new insight of emphysema pathogenesis.
Application : WB
Reactivity : Human
Zhu Y et al. J Biol Chem 2013; 288 (2):1099-113 Modulation of serines 17 and 24 in the LC3-interacting region of Bnip3 determines pro-survival mitophagy versus apoptosis.
Application : WB
Giesert F et al. PLoS One 2013; 8 (5):e63778 Expression analysis of Lrrk1, Lrrk2 and Lrrk2 splice variants in mice.
Application : WB
Unoki M et al. J Biol Chem 2013; 288 (9):6053-62 Lysyl 5-hydroxylation, a novel histone modification, by Jumonji domain containing 6 (JMJD6).
Application : WB
Reactivity : Mouse
Insalaco L et al. J Cell Mol Med 2012; 16 (9):2186-95 analysis of molecular mechanisms and anti-tumoural effects of * acid in breast cancer cells.
Stelter L et al. J Nucl Med 2012; 53 (2):281-6 Novel mechanistic insights into arginine deiminase pharmacology suggest 18F-FDG is not suitable to evaluate clinical response in melanoma.
Kelly MP et al. Br J Cancer 2012; 106 (2):324-32 Arginine deiminase PEG20 inhibits growth of small cell lung cancers lacking expression of argininosuccinate synthetase.
Jablonka S et al. Exp Neurol 2011; 232 (2):261-9 therapeutic effects of pegylated *-like growth factor i in the pmn mouse model of motoneuron disease.
Franco M et al. Blood 2011; 118 (10):2906-17 Pericytes promote endothelial cell survival through induction of autocrine VEGF-A signaling and Bcl-w expression.
Application : WB
Tanaka S et al. J Toxicol Sci 2010; 35 (3):327-34 Salbutamol inhibits lipopolysaccharide-induced inflammatory responses in rat peritoneal macrophages.
Bizzozero OA et al. J Neurosci Res 2009; 87 (13):358549 Identification of major S-nitrosylated proteins in murine experimental autoimmune encephalomyelitis.
Application : WB
Reactivity : Mouse
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399