GeneTex
  • Country / Location Selection

United States (US)

beta Actin antibody [GT5512]

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in IHC (Paraffin sections) (IHC-P). GTX629630

Cat No. GTX629630

Host Mouse
Clonality Monoclonal
Clone Name GT5512
Isotype IgG1
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Rat, Zebrafish, Yeast, Rabbit, Goat, Drosophila, Xenopus
Package
100μl ($319),
25μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

Jurkat , Raji , 293T , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , NIH-3T3 , mouse brain , PC-12 , rat brain , drosophila , yeast , 30 hpf zebrafish , rabbit blood , MDA-MB-231 , goat blood , *L6 , *Ishikawa , *U87-MG

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377426)

Predict Reactivity

Sheep, Bovine, Dog, Chicken, Guinea pig, Japanese Medaka, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Actin Beta,Brws1,Ps1Tp5Bp1,Actb

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody [GT5512] (GTX629630) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 30 µg Jurkat whole cell lysate/extract
B. 30 µg Raji whole cell lysate/extract
C. 30 µg 293T whole cell lysate/extract
D. 30 µg A431 whole cell lysate/extract
E. 30 µg HeLa whole cell lysate/extract
F. 30 µg HepG2 whole cell lysate/extract
G. 30 µg H1299 whole cell lysate/extract
H. 30 µg HCT116 whole cell lysate/extract
I. 30 µg MCF-7 whole cell lysate/extract
J. 30 µg NT2D1 whole cell lysate/extract
K. 30 µg PC-3 whole cell lysate/extract
L. 30 µg U87-MG whole cell lysate/extract
10% SDS-PAGE
Beta Actin antibody [GT5512] (GTX629630) dilution: 1:20000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 30 µg drosophila lysate/extract
10% SDS-PAGE
Beta Actin antibody [GT5512] (GTX629630) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg NIH-3T3 whole cell lysate/extract
C. 30 µg mouse brain lysate/extract
D. 30 µg PC-12 whole cell lysate/extract
E. 30 µg rat brain lysate/extract
10% SDS-PAGE
Beta Actin antibody [GT5512] (GTX629630) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 20 µg 293T whole cell lysate/extract
B. 10 µg 293T whole cell lysate/extract
C. 5 µg 293T whole cell lysate/extract
D. 1 µg 293T whole cell lysate/extract
10% SDS-PAGE
Beta Actin antibody [GT5512] (GTX629630) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 30 µg 30 hpf zebrafish lysate/extract
10% SDS-PAGE
beta Actin antibody [GT5512] (GTX629630) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

beta Actin antibody[GT5512] detects beta Actin protein by western blot analysis.
A. 30 µg MCF-7 whole cell lysate/extract
B. 30 µg MDA-MB-231 whole cell lysate/extract
10% SDS-PAGE
beta Actin antibody[GT5512] (GTX629630) dilution: 1:20000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 30 µg rabbit blood lysate/extract
10% SDS-PAGE
beta Actin antibody [GT5512] (GTX629630) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 30 µg goat blood lysate/extract
10% SDS-PAGE
beta Actin antibody [GT5512] (GTX629630) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629630

GTX629630 ICC/IF Image

beta Actin antibody [GT5512] detects beta Actin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 0.5% Triton X-100 for 1 min, then ice-cold methanol for 5 min.
Green: beta Actin protein stained by beta Actin antibody [GT5512] (GTX629630) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 10 µg yeast lysate/extract
10% SDS-PAGE
beta Actin antibody [GT5512] (GTX629630) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in IHC (Paraffin sections) (IHC-P). GTX629630

GTX629630 IHC-P Image

beta Actin antibody [GT5512] detects beta Actin protein at cell membrane and cytoplasm in rat adrenal gland by immunohistochemical analysis.
Sample: Paraffin-embedded rat adrenal gland.
beta Actin antibody [GT5512] (GTX629630) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
REVIEW

beta Actin antibody [GT5512] Cat No. GTX629630

Rating ( Average 4.7 based on 6 users reviews)
Application
Date : Anonymous submitted on 31-May-2018
Score :
Application Tested : WB
Sample Species : Ms
Sample : Brown fat
Primary Antibody : 1:5000, 4°C, overnightHr
Date : Anonymous submitted on 24-Jun-2015
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : U87-MG cells
Blocking : 5% BSA in PBST, 25°C, 2Hr
Fixation : 4% PFA
Primary Antibody : 1:200, 4°C, overnightHr
Date : Anonymous submitted on 24-Jun-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : U87-MG cells
Amount used : 150μg
Blocking : 5% skim milk in PBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Date : Anonymous submitted on 13-May-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 10^4 cells
Blocking : skim milk, 20°C, 0.5Hr
Primary Antibody : 1:10000, 20°C, 16Hr
Date : Anonymous submitted on 13-Jun-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293 cells, HeLa cells, MCF-7 cells
Amount used : 5μg
Blocking : 3% skim milk in TBS+0.3% tween20, 25°C, 16Hr
Primary Antibody : 1:10000, 25°C, 1Hr
Lane Description : Lane A:HeLa cells, Lane B:MCF-7 cells, Lane C:HEK293 cells
Date : Anonymous submitted on 07-Apr-2014
Score :
Application Tested : WB
Sample : human Ishikawa cells, rat L6 cells
Amount used : 55μg
Primary Antibody : 1:2000, 4°C, overnightHr
Lane Description : Lane1:human Ishikawa cells, Lane2:rat L6 cells
Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169