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beta Actin antibody [GT5512]

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in IHC (Paraffin sections) (IHC-P). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629630
Anti-beta Actin antibody [GT5512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in IHC (Paraffin sections) (IHC-P). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

Cat. No. GTX629630

Host

Mouse

Clonality

Monoclonal

Clone Name

GT5512

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat, Zebrafish, Yeast, Rabbit, Goat, Drosophila, Cat, Dog, Monkey, Xenopus
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

30 hpf zebrafish , Jurkat , Raji , 293T , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , NIH-3T3 , mouse brain , PC-12 , rat brain , rabbit blood , goat blood , MDA-MB-231

Product Note

IP/MS validation was supported by references (PMID:30377426)

Predict Reactivity

Sheep, Bovine, Chicken, Guinea pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2728646

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin beta , BRWS1 , PS1TP5BP1

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody [GT5512] (GTX629630) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg 293T whole cell lysate/extract
D. 30 μg A431 whole cell lysate/extract
E. 30 μg HeLa whole cell lysate/extract
F. 30 μg HepG2 whole cell lysate/extract
G. 30 μg H1299 whole cell lysate/extract
H. 30 μg HCT116 whole cell lysate/extract
I. 30 μg MCF-7 whole cell lysate/extract
J. 30 μg NT2D1 whole cell lysate/extract
K. 30 μg PC-3 whole cell lysate/extract
L. 30 μg U87-MG whole cell lysate/extract
10% SDS-PAGE
Beta Actin antibody [GT5512] (GTX629630) dilution: 1:20000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX629630) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in IHC (Paraffin sections) (IHC-P). GTX629630

GTX629630 IHC-P Image

beta Actin antibody [GT5512] detects beta Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog muscle.
beta Actin stained by beta Actin antibody [GT5512] (GTX629630) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX629630) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629630

GTX629630 ICC/IF Image

beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis.
Sample: PG-4 cells were fixed in ice-cold MeOH for 5 min.
Green: beta Actin stained by beta Actin antibody? (GTX629630) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-beta Actin antibody [GT5512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629630

GTX629630 ICC/IF Image

beta Actin antibody [GT5512] detects beta Actin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 0.5% Triton X-100 for 1 min, then ice-cold methanol for 5 min.
Green: beta Actin protein stained by beta Actin antibody [GT5512] (GTX629630) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 20 μg 293T whole cell lysate/extract
B. 10 μg 293T whole cell lysate/extract
C. 5 μg 293T whole cell lysate/extract
D. 1 μg 293T whole cell lysate/extract
10% SDS-PAGE
Beta Actin antibody [GT5512] (GTX629630) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 30 μg drosophila lysate/extract
10% SDS-PAGE
Beta Actin antibody [GT5512] (GTX629630) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 30 μg goat blood lysate/extract
10% SDS-PAGE
beta Actin antibody [GT5512] (GTX629630) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 30 μg rabbit blood lysate/extract
10% SDS-PAGE
beta Actin antibody [GT5512] (GTX629630) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 30 μg 30 hpf zebrafish lysate/extract
10% SDS-PAGE
beta Actin antibody [GT5512] (GTX629630) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

beta Actin antibody[GT5512] detects beta Actin protein by western blot analysis.
A. 30 μg MCF-7 whole cell lysate/extract
B. 30 μg MDA-MB-231 whole cell lysate/extract
10% SDS-PAGE
beta Actin antibody[GT5512] (GTX629630) dilution: 1:20000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in IHC (Paraffin sections) (IHC-P). GTX629630

GTX629630 IHC-P Image

beta Actin antibody [GT5512] detects beta Actin protein at cell membrane and cytoplasm in rat adrenal gland by immunohistochemical analysis.
Sample: Paraffin-embedded rat adrenal gland.
beta Actin antibody [GT5512] (GTX629630) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg NIH-3T3 whole cell lysate/extract
C. 30 μg mouse brain lysate/extract
D. 30 μg PC-12 whole cell lysate/extract
E. 30 μg rat brain lysate/extract
10% SDS-PAGE
Beta Actin antibody [GT5512] (GTX629630) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 10 μg yeast lysate/extract
10% SDS-PAGE
beta Actin antibody [GT5512] (GTX629630) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

The data was published in the journal Front Cell Neurosci in 2018. PMID: 30337858

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

The data was published in the journal PLoS One in 2014. PMID: 25141004

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

The data was published in the journal PLoS One in 2017. PMID: 28957379

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28169314

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

The data was published in the journal Cell Death Dis in 2013. PMID: 24008737

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

The data was published in the 2021 in Transl Neurosci. PMID: 33708438

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

The data was published in the 2021 in Transl Neurosci. PMID: 33708438

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

The data was published in the journal Toxins (Basel) in 2016. PMID: 27827950

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

The data was published in the journal Toxins (Basel) in 2016. PMID: 27827950

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

The data was published in the journal Front Immunol in 2019. PMID: 31787977

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

The data was published in the journal Cell Discov in 2018. PMID: 30455981

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

The data was published in the journal Sci Rep in 2019.PMID: 30816208

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

The data was published in the journal Front Immunol in 2017.PMID: 29021793

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

The data was published in the journal Oncotarget in 2017.PMID: 28537905

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

The data was published in the journal Sci Rep in 2019.PMID: 30816208

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

The data was published in the journal Genes (Basel) in 2020.PMID: 32085655

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

The data was published in the journal Neuron in 2018.PMID: 29861284

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

The data was published in the journal Sci Rep in 2019.PMID: 30816208

REFERENCE

REVIEW

beta Actin antibody [GT5512] Cat. No. GTX629630

Rating ( Average 4.7 based on 9 users reviews)
Application
Western Blot(WB) ( Average 4.8 based on 8 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 17-May-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : LNCaP and C4-2 cells with/without drug B treatment
Amount used : 3μg
Blocking : 5% BSA in TBST, 24°C, 1Hr
Primary Antibody : 1:10000, 4°C, 16Hr
Date : Anonymous submitted on 14-Feb-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cell lysates
Amount used : 30μg
Blocking : Blocking solution, 4°C, 10Hr
Primary Antibody : 1:3000, 20°C, 4Hr
Date : Anonymous submitted on 15-Dec-2019
Score :
Application Tested : WB
Sample Species : Ms
Sample : Hepa 1-6 cells
Amount used : 10 μg
Blocking : 5% skim milk in TBST 0.1% Tween-20, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 18Hr
Date : Anonymous submitted on 31-May-2018
Score :
Application Tested : WB
Sample Species : Ms
Sample : Brown fat
Primary Antibody : 1:5000, 4°C, 16Hr
Date : Anonymous submitted on 24-Jun-2015
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : U87-MG cells
Blocking : 5% BSA in PBST, 25°C, 2Hr
Fixation : 4% PFA
Primary Antibody : 1:200, 4°C, 16Hr
Date : Anonymous submitted on 24-Jun-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : U87-MG cells
Amount used : 150μg
Blocking : 5% skim milk in PBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 13-May-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 10^4 cells
Blocking : skim milk, 20°C, 0.5Hr
Primary Antibody : 1:10000, 20°C, 16Hr
Date : Anonymous submitted on 13-Jun-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293 cells, HeLa cells, MCF-7 cells
Amount used : 5μg
Blocking : 3% skim milk in TBS+0.3% tween20, 25°C, 16Hr
Primary Antibody : 1:10000, 25°C, 1Hr
Lane Description : Lane A:HeLa cells, Lane B:MCF-7 cells, Lane C:HEK293 cells
Date : Anonymous submitted on 07-Apr-2014
Score :
Application Tested : WB
Sample : human Ishikawa cells, rat L6 cells
Amount used : 55μg
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane1:human Ishikawa cells, Lane2:rat L6 cells
Application Reference
Yeom M et al. Int J Mol Sci 2023; 24 (6)
Application : WB
Reactivity : Human
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations