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beta Actin antibody [GT5512]

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629630
Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630
Anti-beta Actin antibody [GT5512] used in IHC (Paraffin sections) (IHC-P). GTX629630

Cat No. GTX629630

Host

Mouse

Clonality

Monoclonal

Clone Name

GT5512

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat, Zebrafish, Yeast, Rabbit, Goat, Drosophila, Dog, Monkey, Xenopus
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

Jurkat , Raji , 293T , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , NIH-3T3 , mouse brain , PC-12 , rat brain , drosophila , yeast , 30 hpf zebrafish , rabbit blood , MDA-MB-231 , goat blood , *L6 , *Ishikawa , *U87-MG

Product Note

IP/MS validation was supported by references (PMID:30377426)

Predict Reactivity

Sheep, Bovine, Chicken, Guinea pig, Japanese Medaka, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2728646

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

actin beta , BRWS1 , PS1TP5BP1

Cellular Localization

Cytoplasm , cytoskeleton

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (10 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody [GT5512] (GTX629630) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg 293T whole cell lysate/extract
D. 30 μg A431 whole cell lysate/extract
E. 30 μg HeLa whole cell lysate/extract
F. 30 μg HepG2 whole cell lysate/extract
G. 30 μg H1299 whole cell lysate/extract
H. 30 μg HCT116 whole cell lysate/extract
I. 30 μg MCF-7 whole cell lysate/extract
J. 30 μg NT2D1 whole cell lysate/extract
K. 30 μg PC-3 whole cell lysate/extract
L. 30 μg U87-MG whole cell lysate/extract
10% SDS-PAGE
Beta Actin antibody [GT5512] (GTX629630) dilution: 1:20000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 30 μg drosophila lysate/extract
10% SDS-PAGE
Beta Actin antibody [GT5512] (GTX629630) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg NIH-3T3 whole cell lysate/extract
C. 30 μg mouse brain lysate/extract
D. 30 μg PC-12 whole cell lysate/extract
E. 30 μg rat brain lysate/extract
10% SDS-PAGE
Beta Actin antibody [GT5512] (GTX629630) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 20 μg 293T whole cell lysate/extract
B. 10 μg 293T whole cell lysate/extract
C. 5 μg 293T whole cell lysate/extract
D. 1 μg 293T whole cell lysate/extract
10% SDS-PAGE
Beta Actin antibody [GT5512] (GTX629630) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 30 μg 30 hpf zebrafish lysate/extract
10% SDS-PAGE
beta Actin antibody [GT5512] (GTX629630) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

beta Actin antibody[GT5512] detects beta Actin protein by western blot analysis.
A. 30 μg MCF-7 whole cell lysate/extract
B. 30 μg MDA-MB-231 whole cell lysate/extract
10% SDS-PAGE
beta Actin antibody[GT5512] (GTX629630) dilution: 1:20000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 30 μg rabbit blood lysate/extract
10% SDS-PAGE
beta Actin antibody [GT5512] (GTX629630) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 30 μg goat blood lysate/extract
10% SDS-PAGE
beta Actin antibody [GT5512] (GTX629630) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629630

GTX629630 ICC/IF Image

beta Actin antibody [GT5512] detects beta Actin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 0.5% Triton X-100 for 1 min, then ice-cold methanol for 5 min.
Green: beta Actin protein stained by beta Actin antibody [GT5512] (GTX629630) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-beta Actin antibody [GT5512] used in Western Blot (WB). GTX629630

GTX629630 WB Image

beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis.
A. 10 μg yeast lysate/extract
10% SDS-PAGE
beta Actin antibody [GT5512] (GTX629630) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-beta Actin antibody [GT5512] used in IHC (Paraffin sections) (IHC-P). GTX629630

GTX629630 IHC-P Image

beta Actin antibody [GT5512] detects beta Actin protein at cell membrane and cytoplasm in rat adrenal gland by immunohistochemical analysis.
Sample: Paraffin-embedded rat adrenal gland.
beta Actin antibody [GT5512] (GTX629630) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

beta Actin antibody [GT5512] Cat No. GTX629630

Rating ( Average 4.9 based on 23 users reviews)
Application
Western Blot(WB) ( Average 5 based on 22 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2017.PMID: 28537905
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Mk
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2017.PMID: 29021793
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2019.PMID: 30816208
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2019.PMID: 30816208
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Genes (Basel) in 2020.PMID: 32085655
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Neuron in 2018.PMID: 29861284
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2019.PMID: 30816208
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Front Cell Neurosci in 2018.PMID: 30337858
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014.PMID: 25141004
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2017.PMID: 28957379
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2013.PMID: 24008737
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Front Immunol in 2019.PMID: 31787977
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cell Discov in 2018.PMID: 30455981
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Toxins (Basel) in 2016.PMID: 27827950
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Toxins (Basel) in 2016.PMID: 27827950
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28169314
Date : Anonymous submitted on 15-Dec-2019
Score :
Application Tested : WB
Sample Species : Ms
Sample : Hepa 1-6 cells
Amount used : 10 μg
Blocking : 5% skim milk in TBST 0.1% Tween-20, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 18Hr
Date : Anonymous submitted on 31-May-2018
Score :
Application Tested : WB
Sample Species : Ms
Sample : Brown fat
Primary Antibody : 1:5000, 4°C, 16Hr
Date : Anonymous submitted on 24-Jun-2015
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : U87-MG cells
Blocking : 5% BSA in PBST, 25°C, 2Hr
Fixation : 4% PFA
Primary Antibody : 1:200, 4°C, 16Hr
Date : Anonymous submitted on 24-Jun-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : U87-MG cells
Amount used : 150μg
Blocking : 5% skim milk in PBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Date : Anonymous submitted on 13-May-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 10^4 cells
Blocking : skim milk, 20°C, 0.5Hr
Primary Antibody : 1:10000, 20°C, 16Hr
Date : Anonymous submitted on 13-Jun-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293 cells, HeLa cells, MCF-7 cells
Amount used : 5μg
Blocking : 3% skim milk in TBS+0.3% tween20, 25°C, 16Hr
Primary Antibody : 1:10000, 25°C, 1Hr
Lane Description : Lane A:HeLa cells, Lane B:MCF-7 cells, Lane C:HEK293 cells
Date : Anonymous submitted on 07-Apr-2014
Score :
Application Tested : WB
Sample : human Ishikawa cells, rat L6 cells
Amount used : 55μg
Primary Antibody : 1:2000, 4°C, 16Hr
Lane Description : Lane1:human Ishikawa cells, Lane2:rat L6 cells
Application Reference
SDS
PBS.pdf