beta Actin antibody [HL1926]

Name: beta Actin antibody [HL1926]
Brand: GeneTex
SKU: GTX637675
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-beta Actin antibody [HL1926] used in Western Blot (WB). GTX637675

Anti-beta Actin antibody [HL1926] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637675

Anti-beta Actin antibody [HL1926] used in IHC (Paraffin sections) (IHC-P). GTX637675

Cat. No. GTX637675

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1926
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat, Zebrafish, Rabbit, Drosophila, Cat, Dog, African green monkey
Package	100 μl ($399), 25 μl ($169)

See all beta Actin products

Datasheet

View product citations for antibody GTX637675 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:5000-1:50000
ICC/IF	Assay dependent
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Predict Reactivity

Sheep, Bovine, Chicken, Guinea pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human beta Actin

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin beta , BRWS1 , PS1TP5BP1

Cellular Localization

Cytoplasm, cytoskeleton,Nucleus

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. [provided by RefSeq, Aug 2017]

Database

Gene ID: 60 ACTB
UniProt: P60709 ACTB

Research Area

DATA IMAGES

GTX637675 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with beta Actin antibody [HL1926] (GTX637675) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX637675 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody [HL1926] (GTX637675) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX637675 WB Image

GTX637675 ICC/IF Image

beta Actin antibody [HL1926] detects beta Actin protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 10 min.
Green: beta Actin stained by beta Actin antibody [HL1926] (GTX637675) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX637675 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody [HL1926] (GTX637675) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX637675 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody [HL1926] (GTX637675) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX637675 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody [HL1926] (GTX637675) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX637675 IHC-P Image

beta Actin antibody [HL1926] detects beta Actin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
beta Actin stained by beta Actin antibody [HL1926] (GTX637675) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX637675 WB Image

REFERENCE

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REVIEW