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beta Actin antibody [HL1927]

Recombinant antibody
Anti-beta Actin antibody [HL1927] used in Western Blot (WB). GTX637676
Anti-beta Actin antibody [HL1927] used in Western Blot (WB). GTX637676
Anti-beta Actin antibody [HL1927] used in IHC (Paraffin sections) (IHC-P). GTX637676
Anti-beta Actin antibody [HL1927] used in Western Blot (WB). GTX637676
Anti-beta Actin antibody [HL1927] used in Western Blot (WB). GTX637676
Anti-beta Actin antibody [HL1927] used in Western Blot (WB). GTX637676

Cat. No. GTX637676

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1927

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Rabbit, Drosophila, Cat, Dog, African green monkey
Package
100 μl ($399),
25 μl ($169)
See all beta Actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:50000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human beta Actin

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin beta , BRWS1 , PS1TP5BP1

Cellular Localization

Cytoplasm, cytoskeleton,Nucleus

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-beta Actin antibody [HL1927] used in Western Blot (WB). GTX637676

GTX637676 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody [HL1927] (GTX637676) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody [HL1927] used in Western Blot (WB). GTX637676

GTX637676 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody [HL1927] (GTX637676) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody [HL1927] used in IHC (Paraffin sections) (IHC-P). GTX637676

GTX637676 IHC-P Image

beta Actin antibody [HL1927] detects beta Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
beta Actin stained by beta Actin antibody [HL1927] (GTX637676) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Actin antibody [HL1927] used in Western Blot (WB). GTX637676

GTX637676 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody [HL1927] (GTX637676) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody [HL1927] used in Western Blot (WB). GTX637676

GTX637676 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody [HL1927] (GTX637676) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody [HL1927] used in Western Blot (WB). GTX637676

GTX637676 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody [HL1927] (GTX637676) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations