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beta Actin antibody [RM112]

Anti-beta Actin antibody [RM112] used in Western Blot (WB). GTX33610
Anti-beta Actin antibody [RM112] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33610

Cat No. GTX33610

Host Rabbit
Clonality Monoclonal
Clone Name RM112
Isotype IgG
Application WB, ICC/IF, FACS, IP, ELISA, IHC
Reactivity Human
Package
100 μl ($319)
APPLICATION

Application Note

Western Blot (WB): 1:1000 dilution, Immunoprecipitation (IP): 1:200 dilution, Chromatin IP (ChIP): 1:200 dilution, Immunocytochemistry (ICC): 1:200 dilution, Immunohistochemistry (IHC): 1:200 dilution.

Calculated MW

42 kDa. ( Note )

Specificity/Sensitivity

This antibody reacts to β-Actin (Actin, cytoplasmic 1). No cross reactivity with other isoforms of Actin.
PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Immunogen

A peptide corresponding to the N-terminus of β-actin

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Actin Beta,Brws1,Ps1Tp5Bp1,Actb

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-beta Actin antibody [RM112] used in Western Blot (WB). GTX33610

GTX33610 WB Image

Western blot of A431 cells, using anti-β-Actin Clone RM112 at a 1/1000 dilution, showed a band of β-Actin at the predicted MW (42 kDa).

Anti-beta Actin antibody [RM112] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33610

GTX33610 ICC/IF Image

Immunocytochemical staining of HeLa cells, using anti-β-Actin Clone RM112 at 1/200 dilution (red). Nuclei have been labeled with DAPI (blue).

REFERENCE
REVIEW
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Package List Price ($)
$ 319