GeneTex
  • Country / Location Selection

United States (US)

beta Actin antibody - VetSignal

Anti-beta Actin antibody - VetSignal used in Western Blot (WB). GTX134931
Anti-beta Actin antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134931
Anti-beta Actin antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134931
Anti-beta Actin antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134931
Anti-beta Actin antibody - VetSignal used in Western Blot (WB). GTX134931
Anti-beta Actin antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134931

Cat No. GTX134931

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Cat, Dog
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:50000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

MDCK

Predict Reactivity

Human, Mouse, Rat, Rabbit, Sheep, Bovine, Chicken, Guinea pig, Japanese Medaka, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

0.24 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to dog beta Actin.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

actin beta

Cellular Localization

Cytoplasm, cytoskeleton,Nucleus

Database

Research Area

DATA IMAGES

Anti-beta Actin antibody - VetSignal used in Western Blot (WB). GTX134931

GTX134931 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX134931) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134931

GTX134931 IHC-P Image

beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat pancreas.
beta Actin stained by beta Actin antibody (GTX134931) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Actin antibody - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134931

GTX134931 ICC/IF Image

beta Actin antibody detects beta Actin protein at cytoskeleton by immunofluorescent analysis.
Sample: PG-4 cells were fixed in ice-cold MeOH for 5 min.
Green: beta Actin stained by beta Actin antibody (GTX134931) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-beta Actin antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134931

GTX134931 IHC-P Image

beta Actin antibody detects beta Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog muscle.
beta Actin stained by beta Actin antibody (GTX134931) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-beta Actin antibody - VetSignal used in Western Blot (WB). GTX134931

GTX134931 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX134931) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-beta Actin antibody - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX134931

GTX134931 IHC-P Image

beta Actin antibody detects beta Actin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat intestine.
beta Actin stained by beta Actin antibody (GTX134931) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for beta Actin antibody - VetSignal (GTX134931). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for beta Actin antibody - VetSignal (GTX134931). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319